After laser capture microdissection of the nasal cavity transitional cell epithelium RNA was extracted from the control and 120 mg/kg groups (according to the NTP protocol for laser capture microdissected samples (http://www.niehs.nih.gov/research/resources/protocols/extraction/picoscale/index.cfm )). The RNA was analyzed for quantity and purity by UV analysis using the NanoDrop ND-1000 (NanoDropTechnologies, Wilmington, DE). Samples were concentrated using Microcon filters (Millipore, Billerica, MA). All samples were evaluated for RNA integrity by gel electrophoresis using the Flash Gel RNA cassette system (Lonza, Rockland, ME).
Flash gel rna cassette system
Manufactured by Lonza
Sourced in United States
The Flash Gel RNA cassette system is a laboratory instrument designed for the rapid and efficient separation and analysis of RNA samples. It utilizes a precast gel cassette to facilitate the electrophoretic separation of RNA molecules based on their size and charge. The system provides a streamlined and standardized approach to RNA sample preparation and visualization.
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Lab products found in correlation
2 protocols using flash gel rna cassette system
1
Nasal Epithelium RNA Extraction and Analysis
2
Comprehensive RNA Characterization Protocol
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DNA samples and 1 kilobases plus DNA marker were mixed with 6 × loading dye (New England BioLabs, USA), loaded onto a 1% agarose gel in Tris–acetate-EDTA buffer and run at 110 V for 50 min. RNA samples were run and analysed using the FlashGel RNA cassette system (Lonza Bioscience, USA).
Additionally, saRNA samples were prepared according to the Agilent RNA 6000 Nano Kit guide and run on the Agilent Bioanalyzer 2100 using the mRNA Nano assay class protocol. The data was analysed by the 2100 Expert software (B.02.10.S1764). Fraction of intact saRNA based on the electrogram was represented by area under the curve (Supplementary information). The Lonza RNA marker (Lonza Bioscience, USA, #50,577) was used.
Additionally, saRNA samples were prepared according to the Agilent RNA 6000 Nano Kit guide and run on the Agilent Bioanalyzer 2100 using the mRNA Nano assay class protocol. The data was analysed by the 2100 Expert software (B.02.10.S1764). Fraction of intact saRNA based on the electrogram was represented by area under the curve (Supplementary information). The Lonza RNA marker (Lonza Bioscience, USA, #50,577) was used.
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