Protein samples were separated on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred onto polyvinylidene fluoride (PVDF) membrane (Millipore, Billerica, MA). After blocking at room temperature in 5% w/v non-fat milk with TBST buffer (Tris-HCl 10 mM, NaCl 120 mM, Tween-20 0.1%; pH 7.4) for 2 h, membranes were incubated overnight with the appropriate primary anti-collagen I, anti-collagen III, anti-TGF β, anti-Smad2, anti-p-Smad2, anti-CBP, anti-Ser142-p-CREB, anti-Ser133-p-CREB (1∶500, Bioworld Technology, St. Louis Park, MN), anti-tubulin, anti-Lamin B1(1∶1000, Santa Cruz Biotechnology, Santa Cruz, CA), anti-GAPDH (1∶6000, Sigma-Aldrich, St. Louis, MO) at 4°C and then incubated with horseradish peroxidase (HRP)-conjugated secondary antibody at room temperature for 2 h. Proteins were visualized by enhanced chemiluminescence substrate (ECL, Pierce, Rockford, IL).
Anti tgf β
Manufactured by Bioworld Technology
Sourced in United States
Anti-TGF β is a laboratory reagent used for detecting and quantifying Transforming Growth Factor beta (TGF-β) in biological samples. It functions as a specific antibody that binds to TGF-β, allowing for its identification and measurement in various assays.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti tgf β
1
Western Blot Analysis of Extracellular Matrix Proteins
2
Kidney Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Using a homogenate bar, the lower third of each kidney was homogenized after adding
five volumes of homogenate buffer composed of 10 mM HEPES buffer, pH 7.9, 10 mM KCl,
0.1 mM egtazic acid, 1 mM dithiothreitol, and 0.5 mM phenylmethylsulfonyl fluoride.
The kidney homogenates were centrifuged at 3000 g for 15 min at 4°C,
and the supernatants were subsequently stored at -80°C. The protein extracts from the
kidney tissues were separated on 12% SDS-PAGE gels and transferred to polyvinylidene
difluoride membranes. Gapdh served as a loading control.
Bmp7, Igf2, Tgfβ,
Hgf, and Ctgf expression were evaluated using
rabbit polyclonal anti-Bmp7 (1:250; Bioworld Technology, USA),
anti-Hgf (1:500; Abcom, USA), anti-Tgfβ (1:250;
Bioworld Technology), anti-Ctgf (1:3000; Abcom), and
anti-Igf2 (1:150; Bioworld Technology) antibodies, which were
purified for immunogen affinity. The bands were quantified using the Image-Pro Plus
software (USA).
five volumes of homogenate buffer composed of 10 mM HEPES buffer, pH 7.9, 10 mM KCl,
0.1 mM egtazic acid, 1 mM dithiothreitol, and 0.5 mM phenylmethylsulfonyl fluoride.
The kidney homogenates were centrifuged at 3000 g for 15 min at 4°C,
and the supernatants were subsequently stored at -80°C. The protein extracts from the
kidney tissues were separated on 12% SDS-PAGE gels and transferred to polyvinylidene
difluoride membranes. Gapdh served as a loading control.
Bmp7, Igf2, Tgfβ,
Hgf, and Ctgf expression were evaluated using
rabbit polyclonal anti-Bmp7 (1:250; Bioworld Technology, USA),
anti-Hgf (1:500; Abcom, USA), anti-Tgfβ (1:250;
Bioworld Technology), anti-Ctgf (1:3000; Abcom), and
anti-Igf2 (1:150; Bioworld Technology) antibodies, which were
purified for immunogen affinity. The bands were quantified using the Image-Pro Plus
software (USA).
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