3T3-L1 cells were purchased from Zen-Bio (Research Triangle Park, NC, USA). Dulbecco's modified Eagle's medium and other culture reagents were purchased from Thermo Scientific (Rockford, IL, USA). Fatty acid-free bovine serum albumin was purchased from Roche Molecular Biochemicals (Indianapolis, IN, USA). Isobutyl methyl xanthine, dexamethasone, insulin, isoproterenol (ISOP) and 8-Bromo-cyclic adenosine monophosphate (cAMP) were purchased from Sigma (St Louis, MO, USA). Human HDL was obtained as a gift from Dr Jay Heinecke (Department of Medicine, University of Washington, Seattle, WA, USA), and the method of HDL preparation has been published previously.26 (link) Human apoA-I was purified as previously described.27 (link)
8 bromo cyclic adenosine monophosphate camp
Manufactured by Merck Group
Sourced in United States
8-Bromo-cyclic adenosine monophosphate (cAMP) is a synthetic analog of the naturally occurring second messenger cyclic adenosine monophosphate (cAMP). It functions as a signaling molecule that regulates various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Isobutylmethylxanthine, by Merck Group (1 mentions)
Fatty acid free bovine serum albumin, by Roche (1 mentions)
3t3 l1 cells, by ZenBio (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Insulin, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Qunatitect reverse transcription kit, by Qiagen (1 mentions)
Sybr green, by Thermo Fisher Scientific (1 mentions)
Tris edta buffer, by Merck Group (1 mentions)
Medroxyprogesterone 17 acetate, by Merck Group (1 mentions)
Nuclease free water, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
2 protocols using 8 bromo cyclic adenosine monophosphate camp
1
3T3-L1 Adipocyte Differentiation Protocol
2
Quantification of PRL gene expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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8-Bromo-cyclic adenosine monophosphate (cAMP) and medroxyprogesterone 17-acetate (MPA) were obtained from Sigma Aldrich. Nuclease-free water was obtained from Ambion. QunatiTect Reverse Transcription Kit was obtained from QIAGEN. Chloroform, isopropanol and ethanol were obtained from Sigma Aldrich. Tris-EDTA buffer was obtained from Sigma Aldrich. SyBr Green was obtained from Applied Biosystems. Forward and reverse PCR primers for PRL and L19 were obtained from Peprotech.
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