Abemaciclib was purchased from MedChem Express Co. Ltd. (Shanghai, China). IR 780 was purchased from Aladdin Bio‐Chem Technology Co. Ltd. (Shanghai, China). DPPC was purchased from A.V.T. Pharmaceutical Co. Ltd. (Shanghai, China). DSPE‐PEG2000‐NHS (MW = 2978) was purchased from Xi'an Ruixi Biological Technology Co. Ltd. (Xi'an, China). CABT was purchased from Bide Pharmatech Co. Ltd. (Shanghai, China). Cathepsin B sensitive peptide [NH2‐AC(Trt)K(Boc)FR(Pbf)GD(tBu), MW = 1446] was purchased from Dangang Biological Co. Ltd. (Hangzhou, China). Antibodies for flow cytometry were purchased from BioLegend (San Diego, CA, USA). Cathepsin B and methylthiazol tetrazolium (MTT) were purchased from Sigma‐Aldrich (Shanghai, China). The cell cycle analysis, ATP assay, and Annexin V‐FITC/PI apoptosis detection kits were purchased from Beyotime Biotechnology Co. Ltd. (Shanghai, China). The ELISA kits were purchased from Dakewe Co. Ltd. (Shenzhen, China). All the reagents used were of analytical reagent grade.
Cell cycle analysis
Manufactured by Beyotime
Sourced in China
The cell cycle analysis product from Beyotime is a laboratory equipment designed to analyze the cell cycle progression of various cell types. It provides quantitative data on the distribution of cells in different phases of the cell cycle, such as G0/G1, S, and G2/M phases.
Automatically generated - may contain errors
3 protocols using cell cycle analysis
1
Abemaciclib Delivery System Design
2
Cell Cycle Analysis after TOC Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were harvested after 24 hours TOC treatment, and washed once with PBS, then xed with 70% medicinal alcohol for 48 hours. These xed cells prepared for cell cycle analysis (Beyotime Biotechnology, Nanjing, China) were washed with PBS, followed by FACS detection. FlowJo software was applied to data analysis.
3
Cell Cycle Analysis after TOC Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were harvested after 24 hours TOC treatment, and washed once with PBS, then xed with 70% medicinal alcohol for 48 hours. These xed cells prepared for cell cycle analysis (Beyotime Biotechnology, Nanjing, China) were washed with PBS, followed by FACS detection. FlowJo software was applied to data analysis.
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