Calyculin a

MyLa cells were cultured for 24 h and treated with 80 nM Calyculin A (Enzo Life Sciences, Farmingdale, NY, USA #BML-EI192-0025) or DMSO (Control) for 1 h and then, cytoplasmic and nuclear extraction was performed.

MC-LR and MC-RR certified standards, the anti-adda antibody (AD4G2), and the assay kit for detection of MCs/NOD in water by PP2A were purchased from Abraxis, Inc (Warminster, PA, USA). MC-LF, NOD, cantharidin, okadaic acid, and calyculin A standard were purchased from Enzo (Farmingdale, NY, USA). Norcantharidin and phosphate buffered saline, pH 7.4 with Tween 20 (PBS-T) were obtained from Sigma-Aldrich (St. Louis, MO, USA). All MCs and other PP2A inhibitors were stored at −20 °C. The antibody was stored at 4 °C. Acetonitrile was from Pharmaco (Dawsonville, GA, USA). Methanol and formic acid were obtained from Fisher Scientific (Waltham, MA, USA). Life Technologies Corporation (Grand Island, NY, USA) supplied the Dynabeads MyOne Streptavidin T1, Zebaspin 7K molecular weight cut off, 0.5 mL desalting columns, and EZ-link NHS-PEG4-biotin. The Thermomixer C, Protein LoBind 2 mL microcentrifuge tubes, Protein LoBind 1 mL deep well plates, and TwinTec Lo-Bind PCR plates were purchased from Eppendorf (Hauppage, NY, USA). V&P Scientific (San Diego, CA, USA) supplied the 96-well plate magnet (part number VP771HH-MC). The microcentrifuge tube magnet was purchased from Invitrogen (Waltham, MA, USA).