Unless otherwise stated, all chemicals were purchased from Sigma-Aldrich (USA). Compound C was obtained from MedChemExpress (China). 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG) was purchased from the Cayman Chemical Company (USA) and 4',6-diamidino-2-phenylindole (DAPI) was obtained from the Beyotime Institute of Biotechnology (China).
Modena solution was used as the basic medium, which contained 152.8 mmol/L D-glucose, 26.7 mmol/L trisodium citrate, 11.9 mmol/L sodium hydrogen carbonate, 15.1 mmol/L citric acid, 6.3 mmol/L ethylenediamine tetraacetic acid disodium (EDTA-2Na), 46.6 mmol/L tris (hydroxymethyl)aminomethane (Tris), and 4 g/L bovine serum albumin (BSA) (pH=7.2). The Modena solution was supplemented with penicillin G sodium salt (1 000 IU/mL; Solarbio, China), streptomycin sesquisulfate (1 mg/mL; Solarbio, China), and polymyxin B (400 IU/mL; Amresco, USA), and filtered with a 0.22 μm filter to prevent bacterial contamination.
Modena solution was used as the basic medium, which contained 152.8 mmol/L D-glucose, 26.7 mmol/L trisodium citrate, 11.9 mmol/L sodium hydrogen carbonate, 15.1 mmol/L citric acid, 6.3 mmol/L ethylenediamine tetraacetic acid disodium (EDTA-2Na), 46.6 mmol/L tris (hydroxymethyl)aminomethane (Tris), and 4 g/L bovine serum albumin (BSA) (pH=7.2). The Modena solution was supplemented with penicillin G sodium salt (1 000 IU/mL; Solarbio, China), streptomycin sesquisulfate (1 mg/mL; Solarbio, China), and polymyxin B (400 IU/mL; Amresco, USA), and filtered with a 0.22 μm filter to prevent bacterial contamination.