Fluorochrome-conjugated anti-mouse antibodies (anti-CD45.2-PE-Cy7, anti-CD3-APC-Cy7, anti-CD4-APC, anti-CD8-PerCP-Cy5.5, NK1.1-PE, CD11b-APC, Ly6G-PE, Ly6C-FITC, IFNγ APC, TNFα APC-Cy7, anti-CXCR3-PE, Streptavidin-APC and 7-AAD) were purchased from BD PharMingen and eBioscience. AnnexinV FITC was purchased from Biolegend. Trp-2 peptide (Trp2180: SVYDFFVWL) was purchased from Peptide 2.O Inc. Anti-m-OX-40 agonistic Ab (Clone OX-86) and Anti-m-PD-1 antagonistic Ab (Clone: RMP1-14) were purchased from BioXcell. Anti-mouse BLT1 antibody conjugated to biotin was developed in the lab (unpublished data).
Anti cd45.2 pe cy7
Manufactured by BD
Anti-CD45.2-PE-Cy7 is a fluorochrome-conjugated monoclonal antibody that specifically binds to the CD45.2 antigen expressed on the surface of certain cell types. It is designed for use in flow cytometry applications to identify and enumerate these CD45.2-positive cells within a sample.
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Lab products found in correlation
Anti cd4 apc, by BD (1 mentions)
Nk1.1 pe, by BD (1 mentions)
Ifn γ apc, by BD (1 mentions)
Annexin 5 fitc, by BioLegend (1 mentions)
Anti cxcr3 pe, by BD (1 mentions)
Tnfα apc cy7, by BD (1 mentions)
Ly6c fitc, by BD (1 mentions)
Anti cd8 percp cy5, by BD (1 mentions)
Ly6g pe, by BD (1 mentions)
7 aad, by BD (1 mentions)
Streptavidin apc, by BD (1 mentions)
Cd11b apc, by BD (1 mentions)
Anti cd3 apc cy7, by BD (1 mentions)
Anti cd11b apc, by BD (1 mentions)
Anti cd43 pe, by BD (1 mentions)
Anti cd45 apccy7, by Thermo Fisher Scientific (1 mentions)
Streptavidin pe cy7, by Thermo Fisher Scientific (1 mentions)
Fc block anti cd16 cd32, by Thermo Fisher Scientific (1 mentions)
Anti cd45.1 fitc, by BD (1 mentions)
Anti ly6c percp cy5, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti cd45.2 pe cy7
1
Multiparameter Flow Cytometry Analysis
2
Monocyte Identification and Chimerism Analysis
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Erythrocytes were lysed using lysing buffer (BD), and cells were preblocked with anti-CD16/CD32 (Fc Block; eBioscience). Cells were stained on ice for 20 min with combinations of anti-CD45 (APC-Cy7; eBioscience), anti-Ly6C (PerCP-Cy5.5; eBioscience), anti-CD11b (APC; BD), biotinylated anti-CD115 (followed by secondary staining with streptavidin-PE-Cy7; eBioscience), and anti-CD43 (PE; BD) as positive markers and FITC-conjugated lineage markers (CD4, CD19, Ly6G, and Nk1.1; BD) as dump markers for monocytes. For evaluating the reconstitution of chimeric mice, blood cells were stained with anti-CD45.1 (FITC; BD) and anti-CD45.2 (PE-Cy7; BD). Flow cytometry was performed on a FACSCanto II flow cytometer (BD), and FACS data were analyzed with FlowJo Software (Tree Star).
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