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Anti il 1β clone 3a6

Manufactured by Cell Signaling Technology
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Anti-IL-1β (clone 3A6) is a monoclonal antibody that binds to the interleukin-1 beta (IL-1β) protein. IL-1β is a pro-inflammatory cytokine involved in various cellular processes.

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Lab products found in correlation

Dm5500b upright microscope, by Leica (2 mentions) Las image acquisition software, by Leica (2 mentions) Anti β actin clone c4, by Santa Cruz Biotechnology (1 mentions) Enhanced chemiluminescence detection system, by GE Healthcare (1 mentions)

Close spelling variants of this product

IL-1β (253 citations) Anti-IL-1β (83 citations) IL-1β [3A6] (4 citations) Anti-IL-1β (3A6) (2 citations)

3 protocols using anti il 1β clone 3a6

1

Immunohistochemical Staining of IL-1β

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Paraffin embedded biopsies were processed and stained as described.18 (link) Antibodies used were anti-IL-1β (clone 3A6, Cell Signalling Technology, Danvers, MA) and mIgG1 isotype control antibody. Images were acquired using a Leica DM5500B upright microscope and LAS image acquisition software (Leica Microsystems, Rijswijk, the Netherlands).
Veenbergen S., Li P., Raatgeep H., Lindenbergh-Kortleve D., Simons-Oosterhuis Y., Farrel A., Costes L., Joosse M., van Berkel L., de Ruiter L., van Leeuwen M., Winter D., Holland S., Freeman A., Wakabayashi Y., Zhu J., de Ridder L., Driessen G., Escher J., Leonard W, & Samsom J. (2019). IL-10 signaling in dendritic cells controls IL-1β-mediated IFNγ secretion by human CD4+ T cells: relevance to inflammatory bowel disease. Mucosal immunology, 12(5), 1201-1211.
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2

Immunohistochemical Staining of IL-1β

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Paraffin embedded biopsies were processed and stained as described.18 (link) Antibodies used were anti-IL-1β (clone 3A6, Cell Signalling Technology, Danvers, MA) and mIgG1 isotype control antibody. Images were acquired using a Leica DM5500B upright microscope and LAS image acquisition software (Leica Microsystems, Rijswijk, the Netherlands).
Veenbergen S., Li P., Raatgeep H., Lindenbergh-Kortleve D., Simons-Oosterhuis Y., Farrel A., Costes L., Joosse M., van Berkel L., de Ruiter L., van Leeuwen M., Winter D., Holland S., Freeman A., Wakabayashi Y., Zhu J., de Ridder L., Driessen G., Escher J., Leonard W, & Samsom J. (2019). IL-10 signaling in dendritic cells controls IL-1β-mediated IFNγ secretion by human CD4+ T cells: relevance to inflammatory bowel disease. Mucosal immunology, 12(5), 1201-1211.
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3

Quantitative Protein Expression Analysis

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Protein expression in the pulmonary tissues was measured using Western blot analysis as previously described (Chen et al., 2021 (link)). In the present study, the primary antibodies were anti-CC10 (clone EPR19846, 1:2000, Abcam, Cambridge, UK), anti-SP-C (clone EPR19839, 1:2000, Abcam), anti-p-ERK (clone E4, 1:500, Santa Cruz Biotech. Inc, Santa Cruz, CA, United States ), anti-p-p38 (clone E1, 1:500, Santa Cruz Biotech. Inc.), anti-HO-1 (clone P249, 1:2000, Cell Signaling Technology, Danvers, MA, United States ), anti-eNOS (clone M221, 1:2000, Abcam), anti-NRP-1 (clone BGO-14, 1:1000, BosterBio, Pleasanton, CA, United States ), anti-NF-κB (clone D14E12, 1:2000, Cell Signaling Technology), anti-IκBα (clone 44D4, 1:2000, Cell Signaling Technology), anti-TNF-α (clone TN3-19.12, 1:500, Santa Cruz Biotech. Inc.), anti-IL-1β (clone 3A6, 1:2000, Cell Signaling Technology), anti-IL-4 (clone C1, 1:2000, Abcam), and anti-β-actin (clone C4, 1:10,000, Santa Cruz Biotech. Inc.). The membranes were developed using an enhanced chemiluminescence detection system (GE Health Care, Mississauga, Canada). The bands were quantified relative to β-actin bands using ImageJ software.
Tung Y.T., Wei C.H., Yen C.C., Lee P.Y., Ware L.B., Huang H.E., Chen W, & Chen C.M. (2022). Aspirin Attenuates Hyperoxia-Induced Acute Respiratory Distress Syndrome (ARDS) by Suppressing Pulmonary Inflammation via the NF-κB Signaling Pathway. Frontiers in Pharmacology, 12, 793107.
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