U-2 OS-LacO cells (Janicki et al., 2004 (link)) were cultured in DMEM (Sigma-Aldrich) supplemented with 10% FBS (Bodinco BV), 2 mM UltraGlutamine (Lonza), 100 U/ml penicillin, and 100 µg/ml streptomycin (Lonza). HCT116 cells (a kind gift from O. Kranenburg, University Medical Center Utrecht, Utrecht, Netherlands) were cultured in DMEM F12 (Lonza) supplemented with 10% FBS, 2 mM UltraGlutamine, 100 U/ml penicillin, 100 µg/ml streptomycin, and 25 mM Hepes (Lonza). Cell lines were maintained at 37°C and 5% CO2. HCT116 cells stably expressing H2B-mCherry were generated by lentiviral transduction as described before (Hengeveld et al., 2017 (link)). Briefly, HEK293T cells (ATCC, CRL-3216) were cotransfected with pWPT-H2B-mCherry, pRSV, pMD2-G, and pMDLG-I using X-tremeGENE (Roche) according to the manufacturer’s instructions. After 48 h, viruses were harvested and used for viral transduction of HCT116 cells. HCT116 cells expressing doxycycline-inducible CB-INCENPdCEN-mCherry and CB-mCherry were generated by lentiviral transduction as described above and selected on G418. Sf9 cells (ATCC, CRL-1711) were cultured in Insect-EXPRESS (Lonza) supplemented with 5% heat-inactivated FBS, 100 U/ml penicillin, and 100 µg/ml streptomycin. Cells were maintained at 27°C in suspension flasks.
Penicillin
Manufactured by Lonza
Sourced in United States, Switzerland, Belgium, United Kingdom, Germany, France, Italy, Austria, Spain, Netherlands, Canada, Poland, Ireland, Israel, Japan, Brazil
The Penicillin is a piece of lab equipment used for the production and purification of the antibiotic penicillin. It is a specialized device designed to facilitate the fermentation and extraction processes involved in the manufacture of this important pharmaceutical compound.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Lonza (969 mentions)
L glutamine, by Lonza (278 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (149 mentions)
Fetal bovine serum (fbs), by Lonza (115 mentions)
Dulbecco modified eagle medium (dmem), by Lonza (86 mentions)
Streptomycin, by Thermo Fisher Scientific (71 mentions)
Rpmi 1640, by Lonza (70 mentions)
Penicillin, by Thermo Fisher Scientific (67 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (61 mentions)
Amphotericin b, by Lonza (52 mentions)
Fetal bovine serum (fbs), by Merck Group (50 mentions)
Rpmi 1640 medium, by Lonza (48 mentions)
Glutamine, by Lonza (47 mentions)
Hepes, by Lonza (42 mentions)
L glutamine, by Thermo Fisher Scientific (40 mentions)
Rpmi 1640, by Thermo Fisher Scientific (31 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (27 mentions)
Dulbecco s modified eagle s medium dmem, by Lonza (22 mentions)
Non essential amino acids (neaa), by Lonza (22 mentions)
Fetal bovine serum (fbs), by Biowest (22 mentions)
1 050 protocols using penicillin
1
Culturing and Transduction of Cell Lines for Imaging
2
Culturing Various Human and Murine Cell Lines
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A431 human squamous carcinoma cells (CRL-1555; American Type Culture Collection [ATCC], Rockville, MD, USA), MCF7 human breast carcinoma cells (HTB-22; ATCC, Rockville, MD, USA), and MDA-MB-231 human breast carcinoma cells (HTB-26; ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS; Rocky Mountain Biologicals, Missoula, MT, USA), 1% penicillin, and streptomycin (Lonza) on culture dishes. B16BL6 murine melanoma cells (high metastatic variant) (KCLB No. 8006; Korean Cell Line Bank [KCLB], Seoul, Korea) were cultured in minimum essential media alpha (α-MEM; Gibco, Invitrogen, Carlsbad, CA, USA), and B16F1 murine melanoma cells (low metastatic variant) (KCLB No. 8007; Korean Cell Line Bank [KCLB) were cultured in DMEM high glucose (Welgene, Gyeongsan, Korea) supplemented with 10% fetal bovine serum (FBS; Rocky Mountain Biologicals), 1% penicillin, and streptomycin (Lonza, Basel, Switzerland). MCF10A normal-like human breast epithelial cells (CRL-10317; ATCC, Rockville, MD, USA) were cultured in DMEM F-12 (Lonza) supplemented with 10% FBS (Rocky Mountain Biologicals), 1% penicillin, and streptomycin (Lonza). HNF human normal fibroblasts (CC-2512; Lonza) were grown in fibroblast growth medium-2 (FGM-2; Lonza). All cells were incubated at 37 °C in a humidified 5% CO2 environment.
3
Cell Culture Conditions for Multiple Cell Lines
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HeLa, HT-29, NIH-3T3, and L929 cells were purchased from the American Type Culture Collection (USA). HeLa and L929 cells were cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (Gibco), 100 units/ml of penicillin, 100 units/ml of streptomycin (Lonza, Switzerland). HT-29 cells were cultured in McCoy’s 5A medium supplemented with 10% fetal bovine serum (Gibco), 100 units/ml of penicillin, 100 units/ml of streptomycin (Lonza). NIH-3T3 cells were cultured in DMEM supplemented with 10% calf bovine serum (Gibco), 100 units/ml of penicillin, 100 units/ml of streptomycin (Lonza). These cells were maintained at 37°C under an atmosphere of 5% CO2.
4
ADE of Zika and Dengue Virus Infection
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Cell lines tested to study ADE were selected based on previous studies regarding ADE of ZIKV and DENV infection [23 (link),24 (link),25 (link),26 (link),27 (link)]. K562 cells were obtained from ATCC (CCL-243) and were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM; Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Lonza) at 37 °C, 5% CO2. U937 and THP-1 cells (kindly provided by the department of Immunology of Erasmus Medical Center) were cultured in RPMI 1640 (Lonza) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C, 5% CO2. Vero cells (African green monkey kidney epithelial cells, ATCC CCL-81) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 2 mM glutamine. All cell lines were tested and found to be mycoplasma free.
5
Cell Line Maintenance Protocols
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U87MG (GFP or FABP3-GFP) cells were maintained with low glucose (1.0 g/L glucose) DMEM medium (Lonza, Walkersville, MD, USA) supplemented with 2 mM l- glutamine (Lonza), 1% penicillin/streptomycin (100 Units/mL penicillin and 100 µg/mL streptomycin, Lonza), and 10% fetal bovine serum (FBS, Biowest). BT12-GFP cells were maintained with the Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (1:1) growth medium (Gibco) supplemented with 2 mM l- glutamine (Lonza), 1% penicillin/streptomycin (100 Units/mL penicillin and 100 µg/mL streptomycin, Lonza), 15 mM HEPES buffer (Lonza), 2% B27 (Gibco), 0.01 µg/mL recombinant human fibroblast growth factor (FBF-b, Peprotech), 0.02 µg/mL recombinant human epidermal growth factor (EGF, Peprotech), and 1 µg/mL doxycycline. 293FT cells were maintained with high glucose (4.5 g/L glucose) DMEM medium (Lonza) supplemented with 2 mM l- glutamine, 1% penicillin/streptomycin (100 Units/mL penicillin and 100 µg/mL streptomycin), and 10% fetal bovine serum (FBS). All cells were cultured in the humidified incubator at 37 °C under a 5% CO2 atmosphere.
6
SCLC Cell Line Culture Conditions
7
Isolation of Rat Bone Marrow MSCs
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MSCs were isolated from rat bone marrow according to a modified procedure. The single, 12-weeks old rat was provided by the animal facility at the Lebanese American University. It was maintained under optimal laboratory conditions and received food and water ad libidum, complying with the University’s Animal Care and Use Comity (ACUC) and the Guide for the Care and Use of Laboratory Animals [40 ,41 (link)]. The rat was sacrificed by CO2 asphyxiation and both hind legs were aseptically removed. Femoral and tibial bones were then isolated and washed with 70% ethanol and placed in sterile Phosphate Buffered Saline (PBS, Lonza, Basel, Switzerland) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (Lonza). After removing the bone epiphyses with sterile scissors, bone marrows were flushed out using a needle filled with Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (GibcoTM) and 100 U/mL penicillin and 100 μg/mL streptomycin (Lonza). The cells collected were then incubated in vented flasks at 37 °C with 5% CO2. After 5 days of daily medium change, MSCs were identified by their spindle-shaped morphology, as observed using the ZOE Fluorescent Cell Imager [42 (link),43 (link)].
8
Cell Culture Conditions for 293T and MDCK
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293T cells were cultured in DMEM (Lonza, Breda, the Netherlands) supplemented with 10% FCS, 100 IU/ml penicillin (Lonza), 100 µg/µl streptomycin (Lonza), 2 mM glutamine (Lonza), 1 mM sodiumpyruvate (Gibco, Leusden, the Netherlands) and non-essential amino acids (Lonza). Madin-Darby Canine Kidney (MDCK) cells were cultured in EMEM (Lonza) supplemented with 10% FCS, 100 IU/ml penicillin, 100 µg/µl streptomycin, 2 mM glutamine, 1,5 mg/ml sodiumbicarbonate (Lonza), 10 mM hepes and non-essential amino acids.
9
Culturing Primary Rat Cortical Neurons
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Neurons were isolated from 0 to 1 days old postnatal rats of either sex of which brains were placed in cold dissection medium consisting of HBSS (Invitrogen, Carlsbad, CA), 1 mM sodium pyruvate (Sigma‐Aldrich, USA), 0.1% w/v D‐glucose (Riedel‐de Haën, Germany) and 10 mM HEPES (Invitrogen, USA). Cortices were dissociated in DMEM (Invitrogen, USA), containing 2.5% trypsin (Difco Laboratories, USA) and 10 mg/ml DNAse 1 (Roche, Germany) at 37°C for 20 min. Dissociated tissue was passed through a 100 μm cell strainer and centrifuged at 500 g for 5 min. After which the pallet was resuspended in plating medium consisting of DMEM containing pyruvate, glucose and glutamine (Invitrogen, USA), penicillin (100 U/ml, Lonza, Switzerland), streptomycin (100 mg/ml, Lonza, Switzerland) and 10% FCS (Invitrogen, USA). Finally, primary neurons were plated on top of a confluent astrocyte feeder layer consisting of U373 astrocytes. Non‐adhered cells were removed 2 hr after plating. Culturing of primary neurons was performed in culture medium consisting of DMEM (Invitrogen, USA); penicillin (100 U/ml, Lonza, Switzerland), streptomycin (100 mg/ml, Lonza, Switzerland) glutamic acid (25uM),1 mM sodium pyruvate and 10% FCS (Invitrogen, USA) for 12–14 days in a humidified incubator at 37°C and 5% CO2.
10
Kidney Cancer Cell Lines Response to Axitinib
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Human kidney cancer (Caki-2 and A-498) cell lines were purchased from Cell bank Interlab Cell Line Collection (ICLC, Italy) and cultured at 37°C in a humidified atmosphere of 5% CO2. Caki-2 cells were cultured in McCoy's 5a medium (Lonza Bioresearch, Basel, Switzerland) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS), 2mM L-glutamine and 100 IU/ml of penicillin, 100 μg of streptomycin (Lonza). A-498 cells were cultured in EMEM medium (Lonza) supplemented with 10% (v/v) heat-inactivated FBS 2mM L-glutamine and 100 IU/ml of penicillin, 100 μg of streptomycin and 1 mM sodium pyruvate (Lonza).
A-498 and Caki-2 cells were treated with different doses of axitinib (1, 2.5, 5.0, 10.0, 12.5, 25.0, 50.0 and 100 μM) for different times. In some experiments Caki-2 and A-498 cells were pretreated for 1 h with 10 mM of NAC, before axitinib treatment.
A-498 and Caki-2 cells were treated with different doses of axitinib (1, 2.5, 5.0, 10.0, 12.5, 25.0, 50.0 and 100 μM) for different times. In some experiments Caki-2 and A-498 cells were pretreated for 1 h with 10 mM of NAC, before axitinib treatment.
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