Log In Sign up
The largest database of trusted experimental protocols

Phrodo green s aureus bioparticles

Manufactured by Thermo Fisher Scientific
Visit Supplier

The PHrodo Green S. aureus Bioparticles are fluorogenic probes designed to detect the phagocytosis of Staphylococcus aureus particles by cells. The bioparticles exhibit pH-dependent fluorescence, producing a green signal upon acidification within the phagosome during the phagocytic process.

Automatically generated - may contain errors

Lab products found in correlation

Ultra low attachment 96 well plate, by Corning (2 mentions)

Spelling variants of this product

PHrodo green (24 citations) S. aureus (14 citations) S. aureus bioparticles (3 citations) PHrodo S. aureus BioParticles (2 citations) PHrodo Green S. aureus BioParticles phagocytosis kit (2 citations)

5 protocols using phrodo green s aureus bioparticles

1

Phagocytosis Assay for Myeloid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors were harvested and digested (following the protocols described in the sections on harvesting of mouse tissues and flow cytometry), and subjected to myelin removal (Myelin Removal Beads II, human, mouse, rat, ref. 130-096-731). Cell suspensions were then stained as described in the flow cytometry protocol and sorted for CD45+CD11b+ and CD49d+ or CD49d- (MoFlo Astrios EQ). 20,000 FACS-sorted CD49d+/− myeloid cells were then plated in a 96-well plate, allowed to rest for 20 minutes at 37°C, spun down and resuspended in 100μL pHrodo-green S. aureus bioparticles (ThermoFisher, ref. P35367). After 1.5 hours of incubation, GFP high cells were counted on flow cytometry (Gallios, Beckam Coulter).
Chryplewicz A., Scotton J., Tichet M., Zomer A., Shchors K., Joyce J.A., Homicsko K, & Hanahan D. (2022). Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity. Cancer Cell, 40(10), 1111-1127.e9.
+ Open protocol
+ Expand
2

Monocyte Phagocytosis Assay for Trauma Patients

Check if the same lab product or an alternative is used in the 5 most similar protocols
Five separate trauma patient samples and controls were selected to test monocyte phagocytosis. PBMCs were thawed and plated at 100,000 cells/well, then incubated at 37°C for 2 hours with or without the pHrodo Green S. aureus Bioparticles (ThermoFisher Scientific) prepared by the manufacturer’s protocol. After incubation, the cells were washed with PBS, stained with PE/Cy7-labeled CD14 antibody, and analyzed by flow cytometry. Percentage phagocytosis was calculated as
(FITC+PE/Cy7+cellsPE/Cy7+cells)∗100 .
, & Danot O. (2001). A complex signaling module governs the activity of MalT, the prototype of an emerging transactivator family. Proceedings of the National Academy of Sciences of the United States of America, 98(2), 435-440.
+ Open protocol
+ Expand
3

PD-1+ TAM Phagocytosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
20,000 FACS sorted PD-1− and PD-1+ TAMs were plated in an ultra-low attachment 96-well plate (Corning) for 15–20 minutes at 37°C to allow them to rest after sorting. TAMs were then spun down at 1200 g, 5 min, 4°C, and resuspended in 100 μL pHrodo Green S. aureus bioparticles (ThermoFisher) as per the manufacturers instruction. After 2 hours incubation at 37°C, TAMs were spun down and restained with F4/80 in the same fluorescent channel as sorted on, and the phagocytosis assay was subsequently analyzed by flow cytometry. TAMs that were GFP high were considered to be phagocytosing.
Gordon S.R., Maute R.L., Dulken B.W., Hutter G., George B.M., McCracken M.N., Gupta R., Tsai J.M., Sinha R., Corey D., Ring A.M., Connolly A.J, & Weissman I.L. (2017). PD-1 expression by tumor-associated macrophages inhibits phagocytosis and tumor immunity. Nature, 545(7655), 495-499.
+ Open protocol
+ Expand
4

PD-1+ TAM Phagocytosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
20,000 FACS sorted PD-1− and PD-1+ TAMs were plated in an ultra-low attachment 96-well plate (Corning) for 15–20 minutes at 37°C to allow them to rest after sorting. TAMs were then spun down at 1200 g, 5 min, 4°C, and resuspended in 100 μL pHrodo Green S. aureus bioparticles (ThermoFisher) as per the manufacturers instruction. After 2 hours incubation at 37°C, TAMs were spun down and restained with F4/80 in the same fluorescent channel as sorted on, and the phagocytosis assay was subsequently analyzed by flow cytometry. TAMs that were GFP high were considered to be phagocytosing.
Gordon S.R., Maute R.L., Dulken B.W., Hutter G., George B.M., McCracken M.N., Gupta R., Tsai J.M., Sinha R., Corey D., Ring A.M., Connolly A.J, & Weissman I.L. (2017). PD-1 expression by tumor-associated macrophages inhibits phagocytosis and tumor immunity. Nature, 545(7655), 495-499.
+ Open protocol
+ Expand
5

Phagocytosis Assay using Fluorescent Bioparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
pHrodo Green S. aureus Bioparticles (ThermoFisher) were reconstituted as per manufacturer’s instructions to a concentration of 1 mg/ml. The Bioparticles or Fluoresbrite YG 0.5 μM microspheres (Polysciences) were added at a 1:5, or 1:300 ratio, respectively to 1+e6 splenocytes and incubated at 37°C for 1 h. Phagocytosis was stopped by immediately washing cells in ice-cold FACS buffer (1% BSA, 0.01% NaN3, PBS) and placed on ice for further staining.
Larson S.R., Bortell N., Illies A., Crisler W.J., Matsuda J.L, & Lenz L.L. (2020). Myeloid Cell CK2 Regulates Inflammation and Resistance to Bacterial Infection. Frontiers in Immunology, 11, 590266.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!