Antibodies were bought for the detection of PFKFB4 (GeneTex); LC3, PARP (Cell Signaling Technology); β-Actin (Proteintech) and P62 (Abcam). LXX-8250 (95%) was chemically synthesized and obtained from Yuanda Pharmaceuticals (Dalian, China). Z-VAD and 3-MA were from MedChemExpress. Chloroquine diphosphate (CQ) was from Sigma-Aldrich and Dacarbazine was from Topscience. Lactic acid detection kit was from Nanjing Jiancheng Bioengineering Institute, and fructose-1,6-biphosphate (F-1,6-BP) detection kit was from Suzhou Grace Biotechnology.
Chloroquine diphosphate cq
Manufactured by Merck Group
Sourced in United States, United Kingdom
Chloroquine diphosphate (CQ) is a synthetic compound that functions as a laboratory reagent. It is commonly used in various scientific research applications, particularly in the fields of cell biology and immunology. CQ serves as a tool for researchers to study cellular processes and immune system dynamics. The core function of CQ is to provide a chemical substance for experimental purposes, without making claims about its intended use or application.
Automatically generated - may contain errors
Lab products found in correlation
3 methyladenine 3 ma, by Merck Group (4 mentions)
Rapamycin, by Merck Group (3 mentions)
N acetyl l cysteine nac, by Merck Group (2 mentions)
Mg132, by Merck Group (2 mentions)
Zvad fmk zvad, by Merck Group (2 mentions)
Z vad, by MedChemExpress (1 mentions)
Lactic acid detection kit, by Nanjing Jiancheng (1 mentions)
β actin, by Proteintech (1 mentions)
Low melting point agarose, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Hydrazine hydrate, by J&K Scientific (1 mentions)
α sma, by Abways (1 mentions)
Cy5.5 nhs, by Lumiprobe (1 mentions)
Gemcitabine hydrochloride, by Meilun (1 mentions)
Caspase 3, by Abcam (1 mentions)
Gsk 872, by Selleck Chemicals (1 mentions)
Tumour necrosis factor α tnf α, by R&D Systems (1 mentions)
Az628, by Merck Group (1 mentions)
Neratinib, by MedChemExpress (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
34 protocols using chloroquine diphosphate cq
1
Antibody-Based PFKFB4 Protein Detection
2
Gemcitabine and Chloroquine Combination Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gemcitabine hydrochloride was purchased from Meilun Biological Technology (Dalian, China). Chloroquine diphosphate (CQ) and phosphate-PEG5000-NH2 (PEG5K) were purchased from Sigma-Aldrich (USA). The third-generation dendrimer poly-lysine (DGL-G3) was purchased from Colcom (France). Succinic anhydride (SA), 6-phosphonohexanoic acid (6PA), 2-propyl-3-methyl maleic anhydride (CDM) and 4-dimethylaminopyridine (DMAP) were purchased from TCI Chemicals (Shanghai, China). N-hydroxy-succinimide (NHS), 1-[3-(dimethylamino) propyl]-3-ethylcarbodiimide hydrochloride (EDC), ultra-dry N-N-dimethylformamide (DMF), ultra-dry dimethyl sulfoxide (DMSO) and hydrazine hydrate were obtained from J&K Scientific (Beijing, China). Cy5.5-NHS was purchased from Lumiprobe (USA). 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine (DiD) and 3-(4,5-dimethyl-2-tiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were obtained from Beyotime Biotechnology (Beijing, China). 4,6-diamidino-2-phenylindole (DAPI) was purchased from Solarbio Science & Technology (Beijing, China). Low-melting-point agarose was purchased from Gibco (USA). Horseradish peroxidase-conjugated goat anti-rabbit lgG as well as rabbit primary antibodies against GAPDH, paxillin, LC3, p62, MMP2, CD31, α-SMA, and IL-6 were obtained from Abways (Shanghai, China). All other chemicals were of analytical grade.
3
Antibody Sources for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies were purchased from Enzo Biochem (New York, USA; RIP3), BD-Transduction Laboratories (Breda, Netherlands; RIP1), Santa Cruz Biotechnology (Dallas, USA; glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Actin, inhibitor of nuclear factor kappa B (IκBα), Vimentin), Cell Signalling Technology (Danvers, USA; p-ERK, phospho-the c-jun n-terminal kinase (p-JNK), poly (ADP-ribose) polymerase (PARP), Caspase 3, phospho-Receptor-interacting protein kinase 1 (p-RIPK1), NIK), Abcam (Cambridge, UK; p-MLKL, p-RIP3), MLKL, TRIM24, cyclooxygenase-2 (COX2), matrix metalloproteinase 3 (MMP3), matrix metalloproteinase 13 (MMP13)) and Sigma-Aldrich (St Louis, USA; LC3B, Vinculin). Tumour necrosis factor-α (TNF-α) and zVAD were obtained from R&D Systems (Minneapolis, USA). Cycloheximide, Pepstatin A and MG132 were purchased from Calbiochem (San Diego, USA). Chloroquine diphosphate (CQ) and AZ-628 were obtained from Sigma-Aldrich. GSK’872 was purchased from Selleckchem (Houston, USA). Selumetinib was obtained from Abcam. Neratinib was purchased from MedChemExpress (Princeton, USA). HS-1371 was made in-house.28 (link)
4
Culturing and Manipulating NSCLC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human NSCLC cells were cultured in RPMI1640 (NCI-H460, NCI-H1650, and H1299) or in DMEM high glucose medium (A549) and maintained at 37 °C in a humidified atmosphere (95% air and 5% CO2) supplemented with 10% fetal bovine serum, L-Glutamine and antibiotics. H1299 cells expressing shBeclin-1, sh-Control, EGFP-LC3B, or mRFP-EGFP-LC3B were generated in our previous studies [53 (link)]. All cell lines were obtained from Dr. Donatella Del Bufalo and routinely tested for mycoplasma infection by 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, Saint Louis, USA) staining. A-485 was synthetized as previously described [25 (link)], dissolved in DMSO, and stored at −20 °C. For all experiments, cells were treated with 0.1% DMSO, as a control. Chloroquine diphosphate (CQ, Sigma-Aldrich) was dissolved in water and N-Acetyl-l -cysteine (NAC, Sigma-Aldrich) was dissolved in the medium and used freshly.
5
Autophagy Regulation in CHO and HeLa Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This study used two stable CHO-K1 cell lines, CHO/CTRL and CHO/SP1 (generated by transfecting the CHO-K1 cells with pcDNA3.1-CTRL and pcDNA3.1-SP1, respectively) and two stable HeLa cell lines HeLa/CTRL and HeLa/SP1 (generated by transfecting the HeLa cells with pcDNA3.1-CTRL and pcDNA3.1-SP1, respectively). The cells were grown in Iscove’s modified Dulbecco’s medium (IMDM; Thermo Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Young In Frontier, Seoul, Korea) and 1% penicillin and streptomycin (Life Technologies, Carlsbad, CA, USA). G418 (Geneticin; Duchefa Biochemie, Haarlem, Netherlands) was added to the IMDM to achieve a final concentration to 500 μg/mL. The cells were maintained in 25-cm2 T-flasks and were incubated at 37°C in an atmosphere of 5% CO2 to produce monolayer cultures. Starvation was induced by incubating the cells with EBSS (Life Technologies). For autophagy inhibition, 5 mM of 3-Methyladenine (3-MA; Sigma, St. Louis, MO, USA) or 20 μΜ of chloroquine diphosphate (CQ; Sigma) were treated in the absence or presence of EBSS.
6
Chloroquine and Chitosan Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
One milligram (mg) of Chloroquine diphosphate (CQ) (Sigma chemical co.) was dissolved in 10 ml distilled water as stock solution for preparing successive concentrations. Moreover, 0.8 mg of Chitosan (CS) (Sigma -Aldrich co.) was dissolved in a composition of 9.9 (mL) normal saline with 0.1 mL Acetic acid as stock solution for making up next concentrations.
7
Antibody and Inhibitor Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The commercial antibodies for target proteins used in this study were as follows: anti-HA-tag polyclonal antibody (MBL, Nagoya, Japan), anti-HA-tag monoclonal antibody (Cell Signaling Technology, Danvers, MA, USA), anti-MDA5 polyclonal antibody (Abcam, Cambridge, UK), anti-MAVS polyclonal antibody (Abcam), anti-IRF3 polyclonal antibody (Cell Signaling Technology), phospho-IRF3 monoclonal antibody (Cell Signaling Technology), OctA (FLAG)-Probe monoclonal antibody (Santa Cruz Technology, Dallas, TX, USA), anti-mouse IgG (Cell Signaling Technology), anti-rabbit IgG (H+L) secondary antibody (Invitrogen, Meridian Rd., Rockford, IL, USA), anti-mouse IgG (H+L) secondary antibody (Invitrogen), anti-mouse IgG-Fc Fragment antibody (Bethyl Laboratories, Montgomery, Texas, USA) and anti-β-actin polyclonal antibody (Cell Signaling Technology). The chemical reagents used for the inhibitor assay used in this study include: the proteasome inhibitor MG132 (Millipore, Billerica, MA, USA), the lysosome inhibitor, chloroquine diphosphate (CQ; Sigma, St. Louis, MO, USA) and the caspase inhibitor, benzyloxy-carbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD-FMK; Merck, Darmstadt, Germany).
8
Quantifying Cell Viability with CCK-8 Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was assayed using Cell Counting Kit-8 reagent (CCK-8; Nanjing KeyGen Biotech Co., Ltd., Nanjing, China). Briefly, cells were seeded into 96-well plates at 100 cells per well. After overnight growth, cells were treated with or without Chloroquine diphosphate (CQ) (Sigma-Aldrich) for 7 days. The culture medium was replaced with 100 µL RPMI 1,640 supplemented with 10 µL CCK-8 reagent and the cells were incubated for 1 h at 37°C. The absorbance of each well was measured at a wavelength of 450 nm.
9
FMDV Antibodies and Inhibitor Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Monoclonal antibodies directed against DDX21 and PTBP1 were obtained from Abcam (Cambridge, MA, USA). Monoclonal antibodies against HA and Flag tags were obtained from Proteintech (Chicago, IL, USA). Polyclonal pig antiserum against FMDV was produced in our laboratory [60 (link)]. Horseradish peroxidase, tetramethylrhodamine (TRITC) -, and fluorescein isothiocyanate (FITC)-conjugated anti-rabbit/mouse/pig antibodies and chloroquine diphosphate (CQ) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The general caspase inhibitor Z-VAD(OMe)-FMK was acquired from Cell Signaling Technology (Danvers, MA, USA). The proteasomal inhibitor MG-132 was obtained from Selleck Chemicals (Houston, TX, USA). The monoclonal antibody against actin was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). BamHI, EcoRI, and XhoI were obtained from New England Biolabs (NEB, Ipswich, MA, USA).
10
Cytotoxicity Screening of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 57 compounds derived by the VS were purchased by Vitas-M Laboratory (https://vitasmlab.biz/ Radio City, Hong Kong) and by ChemSpace (https://chem-space.com/ , Riga, Latvia). All chemicals were of the highest purity available, as certified by vendor, and were dissolved in DMSO (Sigma-Aldrich, St. Louis, Missouri, USA). For all experiments, cells were treated with < 1% DMSO as control (untreated in figures and figure legends). Cancer cells were exposed to compounds, at concentrations ranging from 5 to 50 µM, for 24 or 72 h. Chloroquine diphosphate (CQ, 5 µM for 72 h, Sigma-Aldrich) and z-VAD-fmk (z-VAD, 50 µM for 72 h, Abcam, Cambridge, UK) were dissolved in water and DMSO, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!