Tnf mice

All studies were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and the Institutional Animal Care and Use Committee guidelines of the University of Washington and Seattle Biomedical Research Institute where all the work involving animals was conducted. C57BL/6 mice were purchased from Charles River, Wilmington, MA. Il1r1^−/− and Tnf^−/− mice were purchased from Jackson Laboratory. Casp1^−/−11^−/− mice were kindly provided by Dr. R. Flavell (Yale University) and described in (Kuida et al., 1995 (link)); Il1a^−/−, Il1ab^−/− mice were described in (Horai et al., 1998 (link)). Il1b^−/− mice were described in (Shornick et al., 1996 (link)). All mice were on C57BL/6 genetic background, matched by age and housed in specific-pathogen-free facilities.

Wild type C57Bl/6 and TNF^-/- mice on the C57Bl/6 background were purchased from Jackson Laboratories (Bar Harbor, ME). IL-17R^-/- mice on the C57Bl/6 background were a gift from Amgen, Seattle [14 (link)]. Mice were immunized with ID93 (0.5 μg) adjuvanted with GLA-SE or GLA-AF (5 μg) via intramuscular injection or intranasal delivery to both nostrils (25 μL total volume). Adjuvants were prepared as described previously [6 (link)]. Mice were anesthetized with ketamine and xylazine prior to intranasal immunization. Mice were immunized 3 times at 3 week intervals. All mice were maintained in specific pathogen-free conditions. After infection animals were maintained in BL3 containment. All procedures were approved by the IDRI institutional animal care and use committee.

Male and female wild type (WT) C57BL/6 mice were purchased from Harlan (Indianapolis, IN), and were given at least 48 hours to acclimate to the vivarium before use. Other mice were obtained as breeder pairs and were bred/maintained under standard conditions in a University of California, San Diego Animal Facility that is accredited by the American Association for Accreditation of Laboratory Animal Care. KRN T cell receptor transgenic mice were a gift from Drs. D. Mathis and C. Benoist (Harvard Medical School, Boston, MA and Institut de Génétique et de Biologie Moléculaire et Cellulaire, Strasbourg, France). These mice were maintained on a C57BL/6 background (K/B). Arthritic mice were obtained by crossing K/B with NOD/Lt (N) animals (K/BxN). Tnf^−/− mice were originally purchased from The Jackson Laboratory (Bar Harbor, Maine, USA).
All animal experiments were conducted according to protocols approved by the Institutional Animal Care and Use Committee of the University of California, San Diego. Mice were housed up to 4 per standard cage at room temperature and maintained on a 12:12 hour light:dark cycle. All behavioral testing was performed during the light cycle. Both food and water were available ad libitum.