Ucp1 cre

All mouse strains used have been previously reported: aP2-Cre (Tang et al., 2008 (link)), Adipoq-Cre (#10803, Jackson Laboratories) (Eguchi et al., 2011 (link)), Ucp1-Cre (#24670, Jackson Laboratories) (Kong et al., 2014 (link)), Fabp4-Cre (#5069, Jackson Laboratories) (He et al., 2003 (link)), Flk1::Cre (Kdr::Cre) (#018976, Jackson Laboratories) (Motoike et al., 2003 (link)), R26-mTmG (#7676, Jackson Laboratories) (Muzumdar et al., 2007 (link)), R26-tdTomato (#7914, Jackson Laboratories) (Madisen et al., 2010 (link)), R26-LacZ (#3474, Jackson Laboratories) (Soriano, 1999 (link)), SmoM2 (#5130, Jackson Laboratories) (Mao et al., 2006 (link)), LSL-Kras^G12D (#8179, Jackson Laboratories) (Jackson et al., 2001 (link)) and Cdkn2A^Flox (Aguirre et al., 2003 (link)). For Kaplan-Meier tumor free survival analysis, Adipoq-Cre;Smo^M2/+ and Ucp1-Cre;Smo^M2/+ animals were observed from birth and confirmed to be tumor free at necropsy. For Kaplan-Meier survival analysis, aP2-Cre; LSL-Kras^G12D and aP2-Cre; LSL-Kras^G12D;Cdkn2a^Flox/Flox animals were observed from birth and sacrificed when showing signs of obvious tumor burden or other distress. Full necropsies were performed. All experimental procedures involving animals were reviewed and approved by SJCRH Institutional Animal Care and Use Committee.

All mice used in this study were C57Bl6/J males. Rictor^floxed (Shiota et al., 2006 (link)), UCP1-Cre (JAX stock 024670), Adiponectin-Cre (JAX stock 010803), and FoxO1^floxed (JAX stock 024756) mice are available from Jackson laboratory. Akt1^floxed and Akt2^floxed mice are described in (Leavens et al., 2009 (link), Wan et al., 2012 (link)). UCP1-CreER is described in (Rosenwald et al., 2013 (link)). Floxed mouse strains were crossed with the different Cre-expressing mice to make tissue specific or inducible tissue specific knockout mice. Cre-negative floxed mice were used as controls. For studies using CreER mice, CreER only mice were included as an additional control.
Unless noted otherwise (e.g. in temperature studies), mice were housed in the UMMS Sherman Center Animal Medicine Facility in a clean room set at 22°C and 45% humidity on a daily 12h light/dark cycle, and kept in ventilated racks fed ad libitum with a standard chow diet, with bedding changed every two weeks.. Mice were sacrificed at 7–30 weeks old depending on the experiment. Please see figure legend for specific age and number of mice used. All animal experiments were approved by the University of Massachusetts Medical School Institutional Animal Care and Use Committee.

Mice carrying floxed LSD1 alleles (KDM1a ^fl/fl), UCP1-Cre, Adiponectin-Cre (AQ-Cre), Myf5-Cre transgenic and Swiss SWR/J mice were obtained from The Jackson Laboratories. aP2-Zfp516 transgenic mice were described previously (Dempersmier et al., 2015 (link)). All protocols for mice studies were approved from the University of California at Berkeley Animal Care and Use Committee. Mice were fed a chow diet or a high fat diet (45% calorie from fat, Research Diet) ad libitum. Body weight and food intake were measured weekly. See also supplemental experimental procedure.