Spd 16 detector

Optical rotations were measured on an MCP 200 polarimeter (Anton Paar, China). Infrared spectroscopy was performed on a Fourier transformation infrared spectrometer coupled with infrared microscope EQUINOX 55 (Bruker, Germany). 1D and 2D NMR data were measured on Bruker Avance 400 or 600 MHz spectrometers (Bruker, Germany) using tetramethylsilane (TMS) as the internal standard. Electrospray mass spectrometry (ESIMS) was obtained on an ACQUITY QDA (Waters Corporation, USA). High resolution electrospray mass spectrometry (HR-ESIMS) was tested on an LTQ-Orbitrap LC–MS spectrometer (Thermo Corporation, USA). Column chromatography was carried out on silica gel with 200–300 mesh (Qingdao Marine Chemical Factory, China) and Sephadex LH-20 (GE Healthcare, UK). High performance liquid chromatography (HPLC) was performed on on Essentia LC-16 with an SPD-16 Detector (Shimadzu, China).

Optical rotations were measured on an MCP 200 polarimeter by using an Na lamp (Shimadzu). UV spectra were obtained on a Blue Star A spectrophotometer. A Fourier transformation infra-red spectrometer coupled with infra-red microscope EQUINOX 55 (Bruker, Rheinstetten, Germany) was used to record the IR spectra. NMR spectra were obtained on a Bruker Avance 400 MHz spectrometer with tetramethylsilane as the internal standard. HR-ESIMS data were obtained on a LTQ-Orbitrap LC-MS spectrometer (Thermo Corporation, Waltham, MA, USA). ESIMS spectra were obtained on an ACQUITY QDA (Waters Corporation, Milford, MA, USA). HPLC was carried out on an Essentia LC-16 with an SPD-16 Detector (Shimadzu, Shanghai, China). Column chromatography was carried out on silica gel (100–200 mesh, 200–300 mesh, Qing dao Marine Chemical Factory, Qingdao, China) and Sephadex LH-20 (GE Healthcare, Littile Chalfont, UK).

Optical rotations were recorded on an MCP 200 (Anton Paar, Shanghai, China) polarimeter. UV data were measured on a Shimadzu UV-240 spectrophotometer (Shimadzu, Kyoto, Japan). IR spectra were detected on a Fourier transformation infra-red spectrometer coupled with infra-red microscope EQUINOX 55 (Bruker, Rheinstetten, Germany). 1D and 2D NMR spectra were performed on a Bruker Avance 400 MHz spectrometer with tetramethylsilane as internal standard. HR-ESIMS data were carried out on an LTQ-Orbitrap LC-MS spectrometer (Thermo Corporation, Waltham, MA, USA). ESIMS spectra were obtained on an ACQUITY QDA (Waters Corporation, Milford, MA, USA). HPLC was carried out on Essentia LC-16 with an SPD-16 Detector (Shimadzu, Shanghai, China). Column chromatography was performed on silica gel (200–300 mesh, Qingdao Marine Chemical Factory, China) and Sephadex LH-20 (GE Healthcare, Littile Chalfont, UK).