Celltitre 96 aqueous one solution cell proliferation assay kit

Manufactured by Promega

Sourced in United States

The CellTiter 96 Aqueous One Solution Cell Proliferation Assay kit is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The assay contains a tetrazolium compound which is bioreduced by cells into a colored formazan product that is soluble in tissue culture medium. The amount of colored product formed is directly proportional to the number of living cells in culture.

Automatically generated - may contain errors

Lab products found in correlation

2 methoxyestradiol, by Merck Group (1 mentions) Tov112d, by American Type Culture Collection (1 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Dylight 800 conjugated goat anti rabbit igg antibody h l, by Thermo Fisher Scientific (1 mentions) Hyclone dmem culture media with and without phenol red, by Thermo Fisher Scientific (1 mentions) Dylight 680 conjugated goat anti rabbit igg antibody h l, by Thermo Fisher Scientific (1 mentions) Alexa 594 donkey anti rabbit secondary, by Jackson ImmunoResearch (1 mentions) Sb203580 p38 mapk inhibitor, by Cayman Chemical (1 mentions) Deadend fluorometric tunel system kit, by Promega (1 mentions) Elisa plate reader, by Tecan (1 mentions) Sunrise microplate reader, by Tecan (1 mentions) Fatal bovine serum, by Thermo Fisher Scientific (1 mentions) Hdac6, by Cell Signaling Technology (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Hdac6, by BPS Biosciences (1 mentions) Amersham ecl select western blotting detection reagent, by GE Healthcare (1 mentions) Dulbecco s modified eagle s medium dmem with l glutamine, by GenDEPOT (1 mentions) Goat anti rabbit igg horseradish peroxidase conjugate, by Santa Cruz Biotechnology (1 mentions) Ac α tubulin, by Cell Signaling Technology (1 mentions) Hdac3, by BPS Biosciences (1 mentions)

Close spelling variants of this product

CellTitre 96 Aqueous One solution (5 citations)

5 protocols using celltitre 96 aqueous one solution cell proliferation assay kit

Cellular Differentiation and Mineralization Assays

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellular viability was determined using the CellTitre 96 Aqueous One Solution Cell Proliferation Assay kit (Promega) according to the manufacturer’s instructions. Alkaline phosphatase activity and staining was detected as previously described42 (link). Calcium deposits were assessed by Alizarin Red S staining27 (link).
For immunofluorescence staining, cells were fixed with 3.7% paraformaldehyde, permeablized with 0.1% Triton X followed by washing and blocking with 10% FBS. Cells were then incubated with the following primary antibodies overnight at 4 °C: Myh2 (1:100) and Myog (1:50), all from DSHB. Samples were incubated with Alexa 488- and 555-conjugated goat anti-mouse IgG (Invitrogen, 1:250) and DAPI stained.

Liu R., Kim K.Y., Jung Y.W, & Park I.H. (2016). Dnmt1 regulates the myogenic lineage specification of muscle stem cells. Scientific Reports, 6, 35355.

+ Open protocol

+ Expand

Comparative Analysis of Cell Line Responses

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HeyC2 cell line [62 (link)] was obtained from Dr. Jean Hurteau at Northshore University Health-Evanston Hospital; TOV112D (CRL11731) cell line was purchased from ATCC. BG1 cells were obtained from Dr. Ken Korach’s lab at NIEHS [63 (link), 64 (link)]. HyClone DMEM culture media (with and without phenol red) from ThermoFisher (SH30604.02); 2-methoxyestradiol from Sigma-Aldrich (M6383); SB203580 p38-MAPK inhibitor from Cayman chemical (13067); DHA (3687) from Tocris Biosciences; 100x HALT protease and phosphatase inhibitor cocktail from ThermoFisher (78440); DyLight™800 conjugated goat anti-rabbit IgG antibody (H&L) (35571) and DyLight™680 conjugated goat anti-rabbit IgG antibody (H&L) (35518) from Thermofisher. Alexa-594 donkey anti-rabbit secondary (133200) from Jackson Immuno Research; DeadEnd Fluorometric TUNEL system kit (G3250) and CellTitre 96® Aqueous one solution cell proliferation assay kit (G3582) were both purchased from Promega (Madison, WI, USA).

Pal P., Hales K., Petrik J, & Hales D.B. (2019). Pro-apoptotic and anti-angiogenic actions of 2-methoxyestradiol and docosahexaenoic acid, the biologically derived active compounds from flaxseed diet, in preventing ovarian cancer. Journal of Ovarian Research, 12, 49.

+ Open protocol

+ Expand

Cell Proliferation Assay with MTS

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cell proliferation rate was measured using the Cell Titre^® 96 Aqueous One Solution Cell Proliferation Assay Kit (Promega, Madison, WI, USA). The cells were seeded at a density of 3000 cells/well in a 96-well plate, 20 μl of MTS (Promega) was added into the medium at different time points and the absorbance (450 nm) was assessed on an ELISA plate reader (Tecan Group Ltd, Männedorf, Switzerland).

Deng B., Wang B., Fang J., Zhu X., Cao Z., Lin Q., Zhou L, & Sun X. (2016). MiRNA-203 suppresses cell proliferation, migration and invasion in colorectal cancer via targeting of EIF5A2. Scientific Reports, 6, 28301.

+ Open protocol

+ Expand

Evaluating AUY922 Cytotoxicity via MTS Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The CellTitre 96 Aqueous One Solution Cell Proliferation Assay kit was utilized as per the manufacturer's instructions (Promega, Fitchburg, WI, USA). Cells were plated in triplicate in a 96-well plate at 30% confluence. The following day, cells were treated with different concentrations of AUY922. The next day, 20 μl of a solution containing 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) was added and cells were incubated for 3 h at 37 °C. This experiment was repeated at four time points (24, 48, 72, and 96 h) to determine the greatest inhibitory effect of the drug. The Sunrise Tecan microplate reader (Tecan Group, Mannedorf, Switzerland) was used to read the plate at 490 nm. Absorbance data was imported into GraphPad Prism where the half maximal inhibitory concentration IC₅₀ was determined by GraphPad software analysis version 6.0 (GraphPad Software, San Diego, CA, USA).

Gild M.L., Bullock M., Pon C.K., Robinson B.G, & Clifton-Bligh R.J. (2015). Destabilizing RET in targeted treatment of thyroid cancers. Endocrine Connections, 5(1), 10-19.

+ Open protocol

+ Expand

HDAC Inhibitor Screening and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dulbecco’s Modified Eagle’s medium (DMEM) with L-glutamine was purchased from GenDEPOT (Barker, TX, USA) and RPMI 1640 medium, fatal bovine serum (FBS), and penicillin/streptomycin were purchased from Gibco BRL (Gaithersburg, MD, USA). Antibodies specific for α-tubulin, Ac-α-tubulin, Histone H3, Ac-Histone H3, PARP, caspase 3, cleaved caspase 8, β-actin HDAC1, and HDAC6 were purchased from Cell Signalling Technology (Boston, MA, USA). Rad52 antibody and goat anti-rabbit IgG horseradish peroxidase conjugate were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Cell Titre 96 Aqueous One Solution cell proliferation assay kit was purchased from Promega (Madison, WI, USA). Amersham ECL select Western blotting detection reagent was purchased from GE Healthcare (Waukesha, WI, USA). HDAC fluorogenic assay kits (HDAC1, HDAC3, HDAC6, and HDAC8) were purchased from BPS Bioscience (San Diego, CA, USA). OxiSelect™ Comet Assay Kit was purchased from Cell Biolabs, Inc (San Diego, CA, USA).

Song Y., Park S.Y., Wu Z., Liu K.H, & Seo Y.H. (2020). Hybrid inhibitors of DNA and HDACs remarkably enhance cytotoxicity in leukaemia cells. Journal of Enzyme Inhibition and Medicinal Chemistry, 35(1), 1069-1079.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!