Analytical grade chemicals

Manufactured by Merck Group

Sourced in Germany, United States, India

Analytical grade chemicals are high-purity chemicals used in scientific and laboratory settings for accurate and reliable analysis. They are designed to meet strict quality standards and have minimal impurities, ensuring consistent and reproducible results. These chemicals are suitable for a wide range of applications, including research, testing, and quality control.

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Lab products found in correlation

31 protocols using analytical grade chemicals

Acetamiprid Toxicity: Sperm and Testes Evaluation

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Acetamiprid (97% of purity) was kindly gifted from Hektaş Ticaret T.A.Ş. (Istanbul, Turkey) and freshly suspended in an aqueous solution of 0.5% methylcellulose (MC) on each study day. Acetamiprid was orally administered to the rats in a fixed fluid volume (4 mL/kg body weight)^{37 (link)}.
Sperm morphology was evaluated using phosphate-buffered saline (PBS 10×), Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM-F12), and fetal bovine serum (FBS) (Multicell Wisent, Quebec, Canada). For histological evaluation of testes, polylysine coated microscope slides and covers (Thermo Scientific, Munich, Germany) and microtome blades (Cologne, Germany), analytical grade chemicals (Sigma Aldrich, Munich, Germany and Merck Millipore, Munich, Germany) were used, while plastic wares from Eppendorf (Nijmegen, Netherlands) and Nest Biotechnology (Munich, Germany).

Arıcan E.Y., Gökçeoğlu Kayalı D., Ulus Karaca B., Boran T., Öztürk N., Okyar A., Ercan F, & Özhan G. (2020). Reproductive effects of subchronic exposure to acetamiprid in male rats. Scientific Reports, 10, 8985.

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Enzymatic Techniques for Molecular Cloning

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Restriction enzymes and Phu DNA polymerase were purchased from New England Biolabs. pENTR/directional TOPO cloning kit (Invitrogen), pQE2 (Qiagen), were procured from the respective sources. Analytical grade chemicals and oligonucleotide primers were procured from Sigma. Malachite green phosphate assay kit (POMG-25H) was purchased from BioAssay System (Gentaur). Electron microscopy reagents were purchased from Electron Microscopy Sciences. Media components were purchased from BD Biosciences. Doxycycline hydrochloride was purchased from Biochem pharmaceutical.

Soni V., Upadhayay S., Suryadevara P., Samla G., Singh A., Yogeeswari P., Sriram D, & Nandicoori V.K. (2015). Depletion of M. tuberculosis GlmU from Infected Murine Lungs Effects the Clearance of the Pathogen. PLoS Pathogens, 11(10), e1005235.

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Preparation of Monoolein-Based Emulsifier

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RYLO MG 20, a commercial grade monoolein emulsifier, comprised predominantly glycerol monoleate (total monoglyceride >95% and bi-, tri-glycerides, and free glycerol <1%), was obtained from Danisco Corporation Ltd. (India). Poloxamer 407, paclitaxel, cisplatin, and poly-Ɛ-lysine were obtained from Sigma-Aldrich (Beijing, China). For each experiment, milli-Q water was utilized. Analytical grade chemicals were obtained from Sigma-Aldrich.

Zhang L., Li J., Tian D., Sun L., Wang X, & Tian M. (2020). Theranostic combinatorial drug-loaded coated cubosomes for enhanced targeting and efficacy against cancer cells. Cell Death & Disease, 11(1), 1.

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LC-MS Solvent Acquisition and Preparation

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Liquid chromatography-mass spectrometry (LC-MS)-grade acetonitrile (ACN) and water with 0.1% formic acid used for LC-MS analyses were acquired from Thermo Fisher Scientific (San Diego, CA, USA). For the extraction process, ethanol and water were supplied by Thermo Fisher Scientific (San Diego, CA, USA), all of high-performance liquid chromatography grade. Analytical-grade chemicals were purchased from Sigma-Aldrich (Steinheim, Germany).

P. Gomes P.W., Mannochio-Russo H., Mao J., Zhao H.N., Ancira J., Tipton C.D., Dorrestein P.C, & Li M. (2024). Co-occurrence network analysis reveals the alterations of the skin microbiome and metabolome in adults with mild to moderate atopic dermatitis. mSystems, 9(3), e01119-23.

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Soybean Embryo Development Profiling

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Soybean (Glycine max L. Merr) cultivar Harosoy63 seeds were used from collections at Agriculture and Agri-Food Canada, and was grown in London research and development center’s field plots. The seeds were planted in two consecutive years (2011 and 2012) to collect two biological replicates of seed samples. Pods were randomly selected from five to seven plants, and developing embryos at 30, 40, 50, 60, and 70 days after pollination (DAP) were excised from the seeds, frozen in liquid nitrogen, and stored at -80°C.
Nicotiana benthamiana seeds were obtained from Dr. Rima Menassa (AAFC-London) and plants were grown in a growth room under a 16 h light at 25°C and 8 h dark at 20°C cycle with light intensity of 80–100 μmol m^-2 sec^-1 and relative humidity of 60–70%. All local, national or international guidelines and legislation were adhered for the use of plants in this study.
The standards of trans-cinnamic acid and p-coumaric acid (HPLC grade) were obtained from Sigma-Aldrich. For HPLC analysis, analytical-grade chemicals were obtained from Sigma-Aldrich and Thermo-Scientific.

Khatri P., Chen L., Rajcan I, & Dhaubhadel S. (2023). Functional characterization of Cinnamate 4-hydroxylase gene family in soybean (Glycine max). PLOS ONE, 18(5), e0285698.

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DNA Cloning and Expression in E. coli

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Analytical-grade chemicals were purchased from Sigma–Aldrich. Enzymes for DNA cloning were purchased from New England Biolabs. The E. coli strains TOP10F’ and BL21(DE3) were purchased from Stratagene. W303 MATa (ura3, ADE2, TRP1, HIS3, LEU2) yeast strains were used.

Peroza E.A., Ewald J.C., Parakkal G., Skotheim J.M, & Zamboni N. (2015). A genetically encoded Förster resonance energy transfer sensor for monitoring in vivo trehalose-6-phosphate dynamics. Analytical biochemistry, 474, 1-7.

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Characterization of Golden Soybean 626

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Soybean (Golden Soybean 626, harvested in 2020) was purchased from Fengyuan Seed Co. Lianyungang, China. The soybean contained 40.52% ± 1.72% protein, 19.57% ± 1.68% lipid, 12.52% ± 0.89% moisture, and 4.40% ± 0.53% ash. Analytical-grade chemicals were purchased from Sigma Aldrich Trading Co Ltd. (Shanghai, China). Deionized water was used for all reagents and sample preparations.

Zou S., Wang Z., Zeng M., He Z, & Chen J. (2024). Improving the Storage Stability of Soy Protein Isolate through Annealing. Foods, 13(4), 615.

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Analytical Chemistry Reagents Sourcing

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Analytical grade chemicals were obtained from Sigma (St. Louis. MO, USA). Solvents were procured from ACI Lab-scan (Bangkok, Thailand). Soybean oil (SBO) was acquired from a local supermarket situated in Hat Yai, Songkhla, Thailand.

Rajasekaran B., Singh A., Nilsuwan K., Ma L., Nazeer R.A, & Benjakul S. (2024). Shrimp oil nanoemulsions prepared by microfluidization and ultrasonication: characteristics and stability. RSC Advances, 14(9), 6135-6145.

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Evaluating Sweet Potato Growth in Martian Regolith

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To assess the impact of Martian regolith on sweet potato growth, tolerance, and nutritional parameters, the Mars Global Simulant (MGS-1) was chosen for this study. The newly formulated MGS-1 soil was preferred because it had been modified to be free of hazardous components, which distinguishes it from other commercially available Mars simulants [19 (link)]. The simulant was procured from the Exolith Laboratory located in Oviedo, FL, USA. All of the analytical-grade chemicals used in this research were sourced from Sigma-Aldrich, St. Louis, MO, USA.

Chinnannan K., Somagattu P., Yammanuru H., Nimmakayala P., Chakrabarti M, & Reddy U.K. (2023). Effects of Mars Global Simulant (MGS-1) on Growth and Physiology of Sweet Potato: A Space Model Plant. Plants, 13(1), 55.

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Synthesis of Highly Crystalline CuO Nanoparticles

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CuO nanoparticles (CuO NPs) were prepared using following analytical–grade chemicals (Sigma Aldrich, Burlington, USA): CuSO₄·5H₂O (0.1 mol dm⁻³), NaOH (1 mol dm⁻³), and sodium poly (4-styrene sulfonate), PSS (w = 0.5 %) without further purification. The stock solutions were prepared by dissolving the CuSO₄·5H₂O and NaOH in deionized water (conductivity <0.055 μs cm⁻¹). CuO was synthesized by adding 0.5 mL NaOH (c (NaOH) = 1.0 mol dm⁻³) to a 2 mL CuSO₄ solution (c (CuSO₄) = 0.1 mol dm⁻³). The prepared solution was heated to 60 °C and stirred magnetically (300 rpm) until the color changed from blue to black. The obtained NPs were separated from the solution by centrifugation at 6000 rpm for 10 min and washed with deionized water in several cycles. The collected material was dried at 100 °C for 5 h. The dried mass was annealed in an oven (Nabertherm, Bremen, Germany) at 400 °C for 2 h to obtain highly crystalline CuO NPs.

Najmi Z., Mlinarić N.M., Scalia A.C., Cochis A., Selmani A., Učakar A., Abram A., Zore A., Delač I., Jerman I., Van de Velde N., Vidmar J., Bohinc K, & Rimondini L. (2023). Antibacterial evaluation of different prosthetic liner textiles coated by CuO nanoparticles. Heliyon, 10(1), e23849.

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