All commercial materials were used without further purification. Flash chromatography was carried out using Macherey-Nagel (Hoerdt, France) Kieselgel 60 M silica. Analytical thin layer chromatography was realized using aluminum-backed plates coated with Macherey-Nagel Kieselgel 60 XtraSIL G/UV254. Compounds were visualized under UV light (at 254 nm) or stained using KMnO4. Nuclear magnetic resonance (NMR) spectra were recorded on a Bruker AVL300 or a Bruker AV400 or a Bruker AV500 spectrometer (Billerica, MA, USA), operating respectively at 300, 400, and 500 MHz for the proton (1H) NMR. Carbon (13C) NMR spectra were recorded on a Bruker AVL300 or a Bruker AV400 spectrophotometer or a Bruker AV500 spectrometer, operating, respectively, at 75, 100, and 125 MHz. Chemical shifts were reported in parts per million (ppm) in the scale relative to residual solvent signals. Multiplicities are abbreviated as follows: s, singlet; d, doublet; t, triplet; dd, doublet of doublets; dt, doublet of triplts, m, multiplet; br, broad. Coupling constants were measured in Hertz (Hz). High-resolution mass spectra (HRMS) and low-resolution mass spectra were performed by the Centre Commun de Spectrométrie de Masse (CCSM), University of Lyon 1, Lyon, France. l - or d -homoserine lactone hydrobromide was synthesized as previously described [20 (link)].
Av400 spectrophotometer
Manufactured by Bruker
Sourced in Germany
The AV400 spectrophotometer is a laboratory instrument manufactured by Bruker. It is designed to measure the absorption or transmission of light by a sample over a specific range of wavelengths. The core function of the AV400 is to analyze the chemical composition and structure of materials through spectroscopic techniques.
Automatically generated - may contain errors
Lab products found in correlation
Av 400, by Bruker (1 mentions)
Avl300, by Bruker (1 mentions)
Av 500 spectrometer, by Bruker (1 mentions)
Micromass q tof mass spectrometer, by Waters Corporation (1 mentions)
Luna c18 column, by Phenomenex (1 mentions)
Agilent 1200 system, by Agilent Technologies (1 mentions)
183 chns analyzer, by Leco (1 mentions)
Flashea 1112 series chns o analyzer, by Thermo Fisher Scientific (1 mentions)
Av 300, by Bruker (1 mentions)
Ftir spectrophotometer, by Bruker (1 mentions)
Av 500 spectrophotometer, by Bruker (1 mentions)
7 protocols using av400 spectrophotometer
1
Synthesis and Characterization of Homoserine Lactone
2
Spectroscopic Characterization of Compounds
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All chemicals and reagents were purchased from Sigma unless otherwise stated. The NMR spectra were recorded on a Bruker AV-400 spectrophotometer (Bruker, Germany) with TMS as internal standard. Chemical shifts (δ) were expressed in ppm with reference to the solvent signals. HR-ESI-MS were determined on a Micromass Q-TOF mass spectrometer (Waters, USA). Analytical HPLC is run on Agilent 1200 system (Agilent, USA) with a Phenomenex Luna C-18 column (250 mm × 4.6 mm, 5 μm, USA). Preparative HPLC was performed on a Wu Feng HPLC system (Shanghai, China) equipped with a preparative reverse phase column (20 × 250 mm, 5 μm).
3
Synthesis of Novel Organic Compounds
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All the starting materials were purchased from Sigma-Aldrich (St. Louis, MO, USA), Daejung (South Korea), and Alfa-Aesar (Germany). Digital Gallenkamp (Sanyo) apparatus was used to record the melting points of final compounds and was uncorrected. Proton NMR (1H NMR) and carbon-13 (13C NMR) spectra were measured on a Bruker AV400 spectrophotometer in CD3OD, CDCl3, and DMSO-d6 at 400 MHz using TMS (Tetramethyl silane) as an internal standard. Alpha Bruker FTIR spectrophotometer (ATR eco ZnSe, νmax in cm−1) was used to record FTIR spectra and elemental analysis was conducted using a LECO-183 CHNS analyzer. All reactions were monitored by thin-layer chromatography (TLC). Merck silica gel HF-254 was used for column chromatographic purification of products using (pet.ether: ethyl acetate, 4:1) as eluent. All chemicals used were of high analytical grade (99% HPLC).
4
NMR Spectroscopy and Elemental Analysis
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1HNMR spectra
were acquired by means of a Bruker AV300 or AV400 spectrophotometer,
using a mixture of DMSO-d6 and CF3COOD (one drop) to facilitate the complete solubilization
of the solids. The 19F{1H}-NMR spectrum of1 was instead recorded in DMSO-d6 after prolonged sonication. The 1H chemical shift values
are referenced to TMS, while the 19F{1H} chemical
shift is referenced to CFCl3. Elemental analyses were performed
on a FlashEA 1112 Series CHNS-O analyzer (ThermoFisher) with gas-chromatographic
separation.
were acquired by means of a Bruker AV300 or AV400 spectrophotometer,
using a mixture of DMSO-d6 and CF3COOD (one drop) to facilitate the complete solubilization
of the solids. The 19F{1H}-NMR spectrum of
are referenced to TMS, while the 19F{1H} chemical
shift is referenced to CFCl3. Elemental analyses were performed
on a FlashEA 1112 Series CHNS-O analyzer (ThermoFisher) with gas-chromatographic
separation.
5
Analytical Characterization of Chemical Compounds
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Starting materials, chemicals,
and solvents of analytical grade were obtained from local traders
from Alfa Aesar, Merck, and Sigma-Aldrich (Germany) and were used
without distillation. Thin-layer chromatography (TLC) was performed
to monitor the reaction using silica gel plates coated with 60 F254
in a mixture of methanol and dichloromethane. UV light was used to
detect the spots on TLC plates. Melting points (mp) were noted using Gallenkamp equipment. FTIR spectra were documented on a
Bruker FTIR spectrometer in KBr pellets. The spectra of 1H NMR and 13C NMR at 400 MHz and 100 MHz (δ = ppm),
respectively, in DMSO-d6 were documented
on a Bruker model AV-400 spectrophotometer.
and solvents of analytical grade were obtained from local traders
from Alfa Aesar, Merck, and Sigma-Aldrich (Germany) and were used
without distillation. Thin-layer chromatography (TLC) was performed
to monitor the reaction using silica gel plates coated with 60 F254
in a mixture of methanol and dichloromethane. UV light was used to
detect the spots on TLC plates. Melting points (mp) were noted using Gallenkamp equipment. FTIR spectra were documented on a
Bruker FTIR spectrometer in KBr pellets. The spectra of 1H NMR and 13C NMR at 400 MHz and 100 MHz (δ = ppm),
respectively, in DMSO-d6 were documented
on a Bruker model AV-400 spectrophotometer.
6
Spectroscopic Characterization of Organic Compounds
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The Gallenkamp (SANYO) model MPD.BM
3.5 apparatus was used to record the melting points. 1H
NMR spectra were measured on a Bruker AV400 spectrophotometer in CD3OD and CD3Cl3 at 300 MHz with tetramethylsilane
(TMS) as the internal standard. Thin-layer chromatography was used
to monitor the reaction progress using silica gel HF-254-coated plates
in different solvent systems with detection by UV-light absorption.
The Alpha Bruker FTIR spectrophotometer (vmax cm–1) was used to measure the FTIR of the synthesized
compounds. All of the chemicals and reagents used in the study were
obtained from Aldrich Chemical Co. Mass spectral analysis was done
using ionization mode MS (EI) with the JEOL 600H-1 instrument.
3.5 apparatus was used to record the melting points. 1H
NMR spectra were measured on a Bruker AV400 spectrophotometer in CD3OD and CD3Cl3 at 300 MHz with tetramethylsilane
(TMS) as the internal standard. Thin-layer chromatography was used
to monitor the reaction progress using silica gel HF-254-coated plates
in different solvent systems with detection by UV-light absorption.
The Alpha Bruker FTIR spectrophotometer (vmax cm–1) was used to measure the FTIR of the synthesized
compounds. All of the chemicals and reagents used in the study were
obtained from Aldrich Chemical Co. Mass spectral analysis was done
using ionization mode MS (EI) with the JEOL 600H-1 instrument.
7
Synthesis and Characterization of Novel Organic Compounds
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All reagents and solvents were purchased from Merck and Aldrich companies without any purification. The reaction progress and the purity of synthesized compounds were monitored by thin-layer chromatography (TLC) using silica gel plates with Fluorescence F254 and UV visualization. Flash chromatography was performed by using a 230–400 mesh silica gel and the indicated solvent system. 1H NMR spectra were recorded on a Bruker AV-400 spectrophotometer at 400 MHz or a Bruker AV-500 spectrophotometer at 500 MHz. 13C NMR spectra were recorded on a Bruker AV-500 spectrophotometer at 125 MHz. Coupling constants (J) are expressed in hertz (Hz). Chemical shifts were reported in parts per million (ppm) relative to an internal standard (tetramethylsilane). Proton coupling patterns were described as singlet (s), doublet (d), triplet (t), quartet (q), multiplet (m), and broad (b). Mass spectra were recorded on an LCMS-Q-TOF (Waters) by means of the ESI method. The elemental analysis for C, H, and N was carried out with an elemental analyzer GmbH VarioEL. Purity of the compound was determined by reverse-phase high-performance liquid chromatography [HPLC, Waters (Column: Waters C18, 5.0 µm, 4.6 × 250 mm) detection wavelength 210 nm; temperature 60°C] to be >95%. A flow rate of 1.0 ml/min was used with the mobile phase of acetonitrile in water with 0.1% modifier (orthophosphoric acid. v/v).
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