Ready to use anti er rabbit monoclonal antibody clone sp1

IHC staining of paraffin-embedded tissue sections was carried out using the Envision System (Dako, Glostrup, Denmark) as described previously^{13 (link)}. The primary antibodies used were as follows: anti-FSCN1 mouse monoclonal antibody (clone 55k-2, diluted at 1:100, Santa Cruz Biotechnology, Santa Cruz, USA); anti-EGFR rabbit polyclonal antibody (clone 1005, diluted at 1:100, Santa Cruz Biotechnology); HercepTest (Dako); ready-to-use anti-ER rabbit monoclonal antibody (clone SP1, Dako); ready-to-use anti-PR mouse monoclonal antibody (clone PR636, Dako).

Tissue Array Preparation: We followed the methods described by Wang et al.^{28 (link)}. In brief, the Quick-Ray^® UT-06 (Unitma Co., Ltd., Seoul, Korea) tissue microarray system and the Quick-Ray premade recipient block (UB-06) wax model were used to prepare tissue specimens (1 mm in diameter). Two representative sites from each breast cancer tissue sample were selected for sampling. IHC Analysis: IHC staining of paraffin-embedded tissue sections used the Envision System (Dako, Glostrup, Denmark), as described previously^{25 (link),26 (link)}. Primary antibodies consisted of anti-WTAP rabbit monoclonal antibody (clone EPR18744, diluted at 1:3200; Abcam, Cambridge, England), ready-to-use anti-ER rabbit monoclonal antibody (clone SP1, Dako), ready-to-use anti-PR mouse monoclonal antibody (clone PgR636, Dako), ready-to-use anti-Podoplanin mouse monoclonal antibody (clone D2-40, Dako) and HercepTest (Dako). The secondary antibody was Dako’s HRP rabbit/mouse universal antibody (Dako, Glostrup, Denmark). The negative control was incubated with vehicle then with secondary antibody, without primary antibody.

IHC staining of paraffin-embedded tissue sections used the Envision System (Dako, Glostrup, Denmark), as described previously [5 (link), 30 (link)]. Primary antibodies consisted of anti-METTL14 mouse monoclonal antibody (clone CL4252, diluted at 1 : 1000; Abcam, Cambridge, England), ready-to-use anti-ER rabbit monoclonal antibody (clone SP1, Dako), ready-to-use anti-PR mouse monoclonal antibody (clone PgR636, Dako), HercepTest (Dako), and ready-to-use anti-Ki-67 mouse monoclonal antibody (clone MIB-1, Dako).