In total, eighteen patients with gout (intermittent phase) or hyperuricemia were included in the current study. The patients with gout fulfilled the 1977 American Rheumatism Association (now the ACR) preliminary criteria for acute arthritis of primary gout and the 2015 ACR/European League Against Rheumatism (EULAR) gout classification criteria. The patients who did not fulfill the gout classification and had a serum urate level above 420 μmol/L were defined as having hyperuricemia. The eighteen patients did not start urate-lowering therapy. Seventeen healthy controls were recruited from the physical examination center. Blood samples from the patients were obtained during a clinic visit, and those of the healthy controls were obtained during a physical examination. Serum was separated within 3 h and stored at − 80 °C. The levels of serum urate and subsequent mouse serum urate and urate in vitro were measured with urate assay kits (Sigma-Aldrich, St Louis, MO, USA). Serum estradiol and mouse serum estradiol were measured with an estradiol ELISA kit (Abcam, Cambridge, MA, USA). This study was approved by the Ethics Committee of the Department of Huashan Hospital, Fudan University (No. 2012-137), and the patients provided written consent for their biological material to be used for research.
Estradiol elisa kit
Manufactured by Abcam
Sourced in United States, United Kingdom
The Estradiol ELISA kit is a quantitative in vitro diagnostic test designed to measure the concentration of estradiol, a type of estrogen, in biological samples such as serum or plasma. The kit uses the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the levels of estradiol present in the samples.
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Lab products found in correlation
Calnexin antibody, by Cell Signaling Technology (1 mentions)
Pdi antibody, by Cell Signaling Technology (1 mentions)
Anti mouse lgg hrp linked antibody, by Cell Signaling Technology (1 mentions)
Anti rabbit lgg hrp linked antibody, by Cell Signaling Technology (1 mentions)
8 ohdg antibody, by Abcam (1 mentions)
Jnk antibody, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
P jnk antibody, by Cell Signaling Technology (1 mentions)
5 protocols using estradiol elisa kit
1
Serum Urate and Estradiol Levels in Gout
2
Plasma Estradiol Quantification by ELISA
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The mice were anesthetized, blood was obtained from the retro-orbital plexus, and plasma was separated. The E2 concentration in the plasma was measured using an enzyme-linked immunosorbent assay (ELISA) kit (estradiol ELISA kit; Abcam, Cambridge, MA) according to the manufacturer’s protocol. Briefly, 25 µL of samples, standard, or control was added into their respective wells, and then 200 µL of E2– horseradish peroxidase conjugate was added to each well. After incubation for 2 hours at 37°C, the content of the wells was aspirated, and each well was rinsed three times with 300 µL of diluted washing solution. Tetramethylbenzidine substrate solution (100 µL) was added into all wells. After 30 minutes of incubation at room temperature in the dark, 100 µL of stop solution was added into all wells and the microplate was shaken gently. The absorbance of the sample was measured at 450 nm within 30 minutes.
3
Endometriosis Patients' IVF and ICSI Protocols
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GC samples were collected from patients (31.8-43.3 years old, mean 37.5 years old) (with or without endometriosis) who underwent in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) at the Affiliated Hospital of Weifang Medical University between April 2013 and December 2018. Endometriosis was diagnosed according to laparoscopic or laparotomy findings. Patients with adenomyosis or malignant neoplasm were excluded. Endometriosis stage was assessed in accordance with the revised American Society for Reproductive Medicine criteria (26 ). Participants that conformed to the following inclusion criteria were included: Basal follicle-stimulating hormone levels of <10 IU/ml, basal estradiol levels of <50 pg/ml, antral follicle count (AFC) of >5 and regular menstrual cycle. At 34-36 h prior to oocyte collection, controlled ovarian stimulation was adopted to perform IVF or ICSI. Basal serum sex hormone levels in patients on days 2-3 of the menstrual cycle was measured using the human follicle stimulating hormone ELISA kit (cat. no. ab108641; Abcam) and estradiol ELISA kit (cat. no. ab108667; Abcam).
4
Estrogen-Induced Oxidative Stress Response
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OVX rat injected E2 (Tocris Bioscience, Bristol, UK; catalog no. 2824), Estradiol ELISA kit (Biovision, Milpitas, CA, USA; catalog no. K7417-100). The primary antibodies used for western blot and immunofluorescence staining were 8-OHDG antibody (Abcam, Cambridge, UK; catalog no. ab48508), BiP antibody (Thermo Fisher Scientific, Lafayette, Colorado; catalog no. PA1-014A), Sigma receptor 1 antibody (Santa Cruz, California, USA; catalog no. sc-137075), pJNK antibody (Cell Signaling Technology, Boston, Massachusetts, USA; catalog no. 4668), JNK antibody (Cell Signaling Technology; catalog no. 9252), PDI antibody (Cell Signaling Technology; catalog no.3501), Ero-1α antibody (Cell Signaling Technology; catalog no.3264), Calnexin antibody (Cell Signaling Technology; catalog no.2679) and β-actin (Cell Signaling Technology; catalog no. 8457). The secondary antibodies used for western blot and immunofluorescence staining were the anti-rabbit lgG, HRP linked antibody (Cell Signaling Technology; catalog no. 7074), anti-mouse lgG, HRP linked antibody (Cell Signaling Technology; catalog no. 7076), Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Thermo Fisher Scientific; catalog no. A32728) and Goat anti-rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Thermo Fisher Scientific; catalog no. A-11029).
5
Quantification of Metabolic Biomarkers
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