Ultrasound and photoacoustic imaging was performed using the Vevo LAZR photoacoustic micro-ultrasound imaging system (FUJIFILM VisualSonics, Toronto, Canada). All images including B-Mode imaging for high-resolution anatomical images, color Doppler imaging for blood flow in cerebral vessels, and photoacoustic imaging for oxygen saturation (SaO2) were generated with the LZ250 transducer at 21 MHz. Parametric maps of oxygen saturation in coronal sections of the brain co-registered with the B-Mode images were generated using a dual-wavelength approach22 . Regions of interest (ROIs) were drawn to encompass the left and right cortical regions of the brain and SaO2 values were plotted over time.
Vevo lazr photoacoustic micro ultrasound imaging system
Manufactured by Fujifilm
Sourced in Canada
The Vevo LAZR photoacoustic micro-ultrasound imaging system is a laboratory equipment manufactured by Fujifilm. The system combines photoacoustic and ultrasound imaging techniques to provide high-resolution visualization of biological samples.
Automatically generated - may contain errors
4 protocols using vevo lazr photoacoustic micro ultrasound imaging system
1
Multimodal Brain Imaging with Photoacoustics
2
Carotid Artery Imaging in Mice
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Mice were anesthetized under 2.5% isoflurane and then placed on a special console (animal handling and physical monitoring platform), and isoflurane concentration was adjusted to the maintenance level of 1.0–1.5%. Mouse ultrasound imaging was performed with the Vevo LAZR photoacoustic micro-ultrasound imaging system (FujiFilm, VisualSonics Inc., Tokyo, Japan). Once the carotid artery was clearly displayed, the probe was adjusted so that the ultrasonic beam was perpendicular to the anterior and posterior walls of the left common carotid artery, and the intima-media of the artery’s anterior and posterior walls was displayed. High-resolution anatomic carotid allograft images were generated with the MS550D transducer at 40 MHz.
3
Characterization of Multifunctional Polymer Gel
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The structure of the polymer was confirmed by Bruker NMR spectrometer (using deuterated DMSO as the solvent). The chemical groups of the composite are characterized by fourier transform infrared spectroscopy (FTIR, PerkinElmer model 1600-FTIR). The rheological properties of the gel were determined by a rheometer (Discovery Hybrid Rheometer (HR2), TA instruments.). The sol-gel transition of the PDNPs-PELA solution is demonstrated by flip test. The morphology of PDNPs in the gel was characterized by FEI Tecnai F20 transmission electron microscope. Photoacoustic imaging was captured using the Vevo LAZR photoacoustic-micro-ultrasound imaging system (FUJIFILM VisualSonics, Toronto, Canada) with a 21 MHz center frequency probe. The particle size was measured using DLS (Malvern, Zetasizer Nano ZS90).
4
Photoacoustic Imaging of Cortical Oxygen Saturation in Rat Pups
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