Cd29 alexafluor700

Manufactured by BioLegend

CD29-AlexaFluor700 is a fluorescently labeled antibody that recognizes the CD29 cell surface marker. CD29 is also known as integrin beta 1 and is involved in cell-cell and cell-matrix interactions. The AlexaFluor700 dye is used to label the CD29 antibody, allowing for its detection in flow cytometry and other fluorescence-based applications.

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Lab products found in correlation

Cd31 pe cy7, by Thermo Fisher Scientific (3 mentions) Cd34 alexafluor647, by BioLegend (3 mentions) Cd45 pe cy7, by Thermo Fisher Scientific (2 mentions) Cl316 243, by Bio-Techne (2 mentions) Live dead blue, by Thermo Fisher Scientific (2 mentions) Cd45 fitc, by Thermo Fisher Scientific (2 mentions) Ps473 akt, by Cell Signaling Technology (2 mentions) Pan akt, by Cell Signaling Technology (2 mentions) Pt308 akt, by Cell Signaling Technology (2 mentions) Antibodies to irβ, by Cell Signaling Technology (2 mentions) Sca1 pacific blue, by BD (2 mentions) Facsdiva software, by BD (2 mentions) Lsrfortessa analyzer, by BD (1 mentions) Collagenase type 2, by Worthington (1 mentions) Cd45 pe cy7, by BioLegend (1 mentions) Cd31 pe cy7, by BioLegend (1 mentions) Fixation permeabilization kit, by Thermo Fisher Scientific (1 mentions) Anti ki67 fitc antibody, by Thermo Fisher Scientific (1 mentions) Cd140a pe, by BioLegend (1 mentions) Lsrii analyzer, by BD (1 mentions)

Spelling variants of this product

AlexaFluor700 anti-mouse/rat CD29 (2 citations)

4 protocols using cd29 alexafluor700

Antibody-based Adipose Tissue Characterization

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Antibodies to IRβ, P-T308-Akt, P-S473-Akt and Pan-Akt (all used at 1/1000 dilution) were from Cell Signaling. UCP1 antibody (#10983) was from AbCam (used at 1/100 for IHC and at 1/1000 for western blot). Live/dead Blue (Molecular Probes, L23105), CD31-PE-CY7 (eBioscience, 25-0311)(used at 1/1000), CD45-PE-CY7 (eBioscience, 25-0451) (used at 1/1000), CD29-AlexaFluor700 (Biolegend, 102218)(used at 1/400), CD34-AlexaFluor647 (Biolegend, 119314)(used at 1/200), Sca1-Pacific Blue (BD Bioscience, 560653)(used at 1/1000), CD45-FITC (eBioscience, 11-0451) (used at 1/200) were used for flow cytometry. CL316,243 was from Tocris.

Sanchez-Gurmaches J, & Guertin D.A. (2014). Adipocytes arise from multiple lineages that are heterogeneously and dynamically distributed. Nature communications, 5, 4099.

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Antibody-based Adipose Tissue Characterization

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Sanchez-Gurmaches J, & Guertin D.A. (2014). Adipocytes arise from multiple lineages that are heterogeneously and dynamically distributed. Nature communications, 5, 4099.

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Adipose Tissue Stromal Cell Isolation

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Isolation of adipose tissue stromal cells was performed essentially as described20 (link). Briefly, adipose tissue was excised, minced, and digested in Hank’s Balanced Salt Solution (HBSS) (Sigma no. H8264) containing 3% BSA and 0.8 mg/ml Collagenase Type 2 (Worthington Biochemical; LS004174) for 75 min in a shaking water bath at 37 °C. The mixture was then filtered through a 40 μm filter, and filtered cells were pelleted and washed in HBSS containing 3% BSA. For Ki67 analysis, cells were stained with the following antibodies at room temperature: CD45 PE-Cy7 (Biolegend; clone 30-F11), CD31 PE-Cy7 (Biolegends; clone 390), CD29 Alexa Fluor 700 (BioLegend; clone HMβ1-1), CD34 Alexa Fluor 647 (BioLegend; clone MEC14.7) and CD140a PE (Biolegend; clone APA5). Anti-Ki67 FITC antibody (eBioscience, clone SOLA15) was used overnight at 4 °C. Cells
were washed, fixed and permeabilized using the Fixation/Permeabilization kit (eBioscience) as per manufacturer’s protocol. All antibody incubations and washing steps were performed in HBSS with 3% BSA. Following antibody incubation, samples were analyzed on a BD LSR Fortessa analyzer. Data analysis was performed using BD FACS Diva software (BD Biosciences).

Wagner G., Lindroos-Christensen J., Einwallner E., Husa J., Zapf T.C., Lipp K., Rauscher S., Gröger M., Spittler A., Loewe R., Gruber F., Duvigneau J.C., Mohr T., Sutterlüty-Fall H., Klinglmüller F., Prager G., Huppertz B., Yun J., Wagner O., Esterbauer H, & Bilban M. (2017). HO-1 inhibits preadipocyte proliferation and differentiation at the onset of obesity via ROS dependent activation of Akt2. Scientific Reports, 7, 40881.

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Flow Cytometry Analysis of Stem Cell Markers

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Flow cytometry was performed as described previously (Jeffery et al. 2015 (link)) for BrdU analysis with the following antibodies: CD45 APC-eFluor 780 (eBioscience; 47–0451–80) at 1:1,000, CD31 PE-Cy7 (eBioscience, 25–0311–82), at 1:500, CD29 Alexa Fluor 700 (BioLegend, 102218) at 1:400 and Sca-1 V500 (BD Horizon, 561228) at 1:300. Cells were washed and then fixed and permeabilized using Phosflow lyse/fix and Perm Buffer III (BD Biosciences) according to the manufacturer’s recommendations. Cells were then treated with DNase (deoxyribonuclease I; Worthington; × 200 units/ml) in DPBS (Sigma; with calcium chloride and magnesium chloride) for 2-hrs at 37C and then washed in HBSS with 3% BSA. Cells were then stained with anti-BrdU antibody (Alexa Fluor 647; Phoenix Flow Systems; AX647) at 1:30 in HBSS with 3% BSA overnight in the dark at 4C. Cells were then washed in HBSS with 3% BSA and incubated with CD34 Brilliant Violet 421 (BioLegend 230 119321) at 1:400 and CD24 PerCP-Cyanine 5.5 (eBioscience, 45–0242–80) at 1:250. Following antibody incubation, samples were washed and analyzed on a BD LSRII analyzer. Data analysis was performed using BD FACS Diva software (BD Biosciences).

Saavedra-Peña R.D., Taylor N, & Rodeheffer M.S. (2022). Insights of the role of estrogen in obesity from two models of ERα deletion. Journal of molecular endocrinology, 68(4), 179-194.

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