Ethyl acetate

Glycerol (purity ≥ 99.0%), ethanol (purity ≥ 99.7%), ethyl acetate (purity ≥ 99.5%) and phosphoric acid (purity ≥ 85.0%) were purchased from Tianjin Damao Chemical Reagent Co. (China). Lauric acid (purity ≥ 98.0%), SB (purity ≥ 99.0%), PS (purity ≥ 99.0%), and sodium hydroxide (purity ≥ 98.0%) were obtained from Aladdin Biochemical Technology Company (China). Lipozyme 435 was obtained from Novozymes Co. (Denmark). All water is purified by Mili-Q (Advantage A10, Merck KgaA, Germany).

β-D-glucosidase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China; methanol (chromatographic pure), ethyl acetate (chromatographic pure), citric acid, disodium hydrogen phosphate, and anhydrous sodium carbonate were obtained from Tianjin Damao Chemical Reagent Factory, Tianjin, China; concentrator plus vacuum concentrator, ultra-low temperature refrigerator, electronic balance, ultrasonic cleaner, pipette gun were obtained from Eppendorf, Hamburg, Germany; thermostatic bath was obtained from PolyScience, Niles, IL, USA; acid meter, Trace1300 gas chromatograph, Trace ISQ mass spectrometer (GC-MS), and NanoDrop 2000 nucleic acid analyzer were obtained from Thermo, Waltham, MA, USA; high-efficiency plant total RNA extraction kit, UnionScript First-strand cDNA synthesis mix for qPCR kit, and real-time fluorescence quantitative SYBR GreenⅠMaster kit were purchased from Beijing Jinsha Biological Co., Ltd., Beijing, China; LightCycler 480 quantitative PCR instrument was obtained from Roche, Basel, Switzerland.

Coptis chinensis Franch.(Huanglian), Scutellaria baicalensis Georgi (huangqin), Phellodendron amurense Rupr.(huangbai), and Gardenia jasminoides J. Ellis (zhizi) were purchased from Hangzhou Mintai (Bozhou) Chinese Herbal Medicine Co. Ltd. (Zhejiang, China) and were certified by the standards elaborated in Chinese Pharmacopeia (2020 edition). Portions of the above four herbs were deposited in the Department of Pharmacy of Taizhou Central Hospital (Taizhou University Hospital), Taizhou, Zhejiang for future reference under reference number Xu1-4. 2,4-dinitrofluorobenzene (DNFB) was purchased from Shanghai McLean Biochemical Technology Co., Ltd. (Shanghai, China).
MEthanol, acetonitrile, and formic acid (LC-MS grade) were purchased from CNW Technologies (Germany). Ethanol, petroleum ether, and ethyl acetate (analysis grade) were obtained from Tianjin Damao Chemical Reagent Co., Ltd. (China). The standard substances of baicalin, chrysin, ferulic acid, geniposide, obacunone, wogonin, wogonoside, auraptene, protopine, isochlorogenic acid A, chelerythrine, rutecarpine, palmatine, and berberine were purchased from the resource platform of the National Standard Material (China).