Mitoprobe jc 1 kit

Ferruginol and synthetic analogues (Figure 1) were synthesised as described previously by one of the authors (M. Gonzalez-Cardenete): compounds 1 and 2 [47] and compounds 3-11 [19] (link). The characterisation data for all tested compounds were in excellent agreement with those reported by us [19, (link)47] and similar purity, above 95% (NMR data are available on request). All other chemicals were from Sigma-Aldrich (St. Louis, MO, USA) Sulforhodamine B, trichloroacetic acid, Trizma base, propidium iodide, Ribonuclease A, formaldehyde and crystal violet. Glacial acetic acid, ethanol and methanol were from Fisher (Leicestershire, UK). Minimum essential media (MEM), heat-inactivated fetal bovine serum (FBS), penicillin-streptomycin antibiotic, non-essential amino acids solution (NEAA), sodium pyruvate, TrypLE Express (1×, trypsin, EDTA, phenol red), phosphate-buffered saline (PBS), ReadyProbes ® cell viability imaging kit, trypan blue and the MitoProbe JC-1 kit were from Thermo Fisher Scientific (Waltham, MA, USA). Matrigel was from BD Bioscience (San Jose, CA, USA), Caspase-Glo ® 3/7 from Promega and DAPI staining from Cell Signalling Technology (Danvers, MA, USA). CytoSelect Cell Migration Assay kit was from Cell Biolab Inc (San Diego, CA, USA).