The fibrous morphology of the sheath-core structured membranes was assessed using transmission electron microscopy (TEM) (JEOL JEM-2000EXII, Japan). A Leica TS SP8X laser-scanning confocal microscope (Tokyo, Japan) was employed to confirm the presence of proteins in the sheath/core structured nanofibers, with PLGA as the sheath layer and recombinant enhanced green fluorescent protein (reGFP) as the core. The excitation wavelength for observing the co-axial electrospun nanofibrous membranes was set at 487 nm.
Ts sp8x laser scanning confocal microscope
Manufactured by Leica
Sourced in Japan
The TS SP8X laser-scanning confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It features a modular architecture and a range of laser options to provide flexibility and precision in sample analysis.
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Lab products found in correlation
2 protocols using ts sp8x laser scanning confocal microscope
1
Characterization of Sheath-Core Nanofibers
2
Characterization of Electrospun Nanofibers
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The morphological structure of the electrospun nanofibers was evaluated by means of scanning electron microscopy (SEM). To assess the nanofiber diameter distribution, 100 fibers were randomly selected (N = 3), and an ImageJ code (National Institutes of Health, Bethesda, MD, USA) was used.
A JEOL Model JEM-2000EXII transmission electron microscope (TEM) from Tokyo, Japan, was used to confirm the sheath/core structure of coaxial electrospun nanofibers. To verify the presence of active biomolecules, PLGA was used as the sheath material and reGFP at the core in the electrospinning process. The resulting nanofibers were observed under a Leica TS SP8X laser scanning confocal microscope (LSCM) from Tokyo, Japan, at a wavelength of 487 nm.
A JEOL Model JEM-2000EXII transmission electron microscope (TEM) from Tokyo, Japan, was used to confirm the sheath/core structure of coaxial electrospun nanofibers. To verify the presence of active biomolecules, PLGA was used as the sheath material and reGFP at the core in the electrospinning process. The resulting nanofibers were observed under a Leica TS SP8X laser scanning confocal microscope (LSCM) from Tokyo, Japan, at a wavelength of 487 nm.
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