Human embryonic kidney cell line 293 (HEK293) was kindly provided by Dr. Joseph Ruiz at Enzerna Biosciences (Raleigh, NC). Media and reagents for growing and maintaining cells were purchased from Life Technologies Corporation (Carlsbad, CA). The cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% (by volume) fetal bovine serum and 1% penicillin-streptomycin. Cells were sustained in a humidified incubator at 37ºC and 5% CO2. A 0.25% trypsin-EDTA solution was used for detachment of cells. One Shot Stbl3 Chemically Competent cells of Escherichia coli (Life Technologies Corporation) were used for constructing the mutagenesis plasmid. Bacteria carrying the plasmids were maintained in Luria Bertani (LB; Sigma-Aldrich, St. Louis, MO) agar or broth, and sustained in a humidified incubator at 37ºC.
One shot stbl3 chemically competent cells of escherichia coli
Manufactured by Thermo Fisher Scientific
One Shot Stbl3 Chemically Competent cells of Escherichia coli are a laboratory product designed for bacterial transformation. They are chemically treated Escherichia coli cells that can efficiently take up and maintain plasmid DNA.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using one shot stbl3 chemically competent cells of escherichia coli
1
HEK293 Cell Culture and Plasmid Transformation
2
HEK293 Cell Culture and Plasmid Transformation
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Human embryonic kidney cell line 293 (HEK293) was kindly provided by Dr. Joseph Ruiz at Enzerna Biosciences (Raleigh, NC). Media and reagents for growing and maintaining cells were purchased from Life Technologies Corporation (Carlsbad, CA). The cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% (by volume) fetal bovine serum and 1% penicillin-streptomycin. Cells were sustained in a humidified incubator at 37 °C and 5% CO2. A 0.25% trypsin-EDTA solution was used for detachment of cells. One Shot Stbl3 Chemically Competent cells of Escherichia coli (Life Technologies Corporation) were used for constructing the mutagenesis plasmid. Bacteria carrying the plasmids were maintained in Luria Bertani (Sigma-Aldrich, St. Louis, MO) agar or broth, and sustained in a humidified incubator at 37 °C.
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