Sfa 1

Manufactured by ZenBio

The Sfa-1 is a compact and versatile spectrophotometer designed for a wide range of laboratory applications. It utilizes a high-intensity LED light source and a photo-detector to measure the absorbance or transmittance of samples across a broad wavelength range. The Sfa-1 is capable of performing accurate and reproducible measurements with a simple and intuitive user interface.

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Lab products found in correlation

Ab108785, by Abcam (2 mentions) E91827mu, by USCN (2 mentions) Human specific netrin 1 elisa, by USCN (2 mentions) Ezrmi 13k, by Crystal Chem (1 mentions) Ezrmi 13k, by Merck Group (1 mentions) Triglycerides reagent, by Thermo Fisher Scientific (1 mentions) Tr71121, by Thermo Fisher Scientific (1 mentions) Tr70121, by Thermo Fisher Scientific (1 mentions)

6 protocols using sfa 1

Serum Biomarker Measurement in Mice

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Serum collected from fasted, re-fed, and/or ad-libitum fed mice was used to measure insulin (Millipore #EZRMI-13K), leptin (Crystal Chem #90030), adiponectin (Thermo Fisher #KMP0041), and free fatty acids (ZenBio #sfa-1) as noted.

Felix J.B., Saha P.K., de Groot E., Tan L., Sharp R., Anaya E.S., Li Y., Quang H., Saidi N., Abushamat L., Ballantyne C.M., Amos C.I., Lorenzi P.L., Klein S., Gao X, & Hartig S.M. (2024). N-acetylaspartate from fat cells regulates postprandial body temperature. Research Square.

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Quantifying Metabolic Biomarkers in Serum

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Insulin (90080; crystal Chem Inc), adiponectin (ab108785; Abcam), FFA (SFA-1; Zen Bio), TNFa (88-7064-76; ebiosciences) and netrin-1 (E91827Mu; USCN life science Inc) levels were measured in the serum or cell supernatants using mouse standards according to manufacturer’s guidelines. Netrin-1 in human serum was measured using a human-specific netrin-1 ELISA from USCN Life Science Inc (700176; mybiosource).

Ramkhelawon B., Hennessy E.J., Ménager M., Ray T.D., Sheedy F.J., Hutchison S., Wanschel A., Oldebeken S., Geoffrion M., Spiro W., Miller G., McPherson R., Rayner K.J, & Moore K.J. (2014). Netrin-1 promotes adipose tissue macrophage accumulation and insulin resistance in obesity. Nature medicine, 20(4), 377-384.

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Metabolic Markers in Serum and Liver

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Fed serum levels were used to measure insulin (#EZRMI-13K; Millipore), leptin (#90030; Crystal Chem), adiponectin (#KMP0041; Thermo Fisher), and free fatty acids (#sfa-1; ZenBio), and FGF21 (#MF2100; R&D Systems). Insulin levels during glucose tolerance tests were also measured by ELISA (Millipore). Hepatic triglyceride (TG) content was quantified by Thermo Fisher Scientific Triglycerides Reagent (#TR22421) and normalized per gram of liver tissue.

Cox A.R., Chernis N., Kim K.H., Masschelin P.M., Saha P.K., Briley S.M., Sharp R., Li X., Felix J.B., Sun Z., Moore D.D., Pangas S.A, & Hartig S.M. (2021). Ube2i deletion in adipocytes causes lipoatrophy in mice. Molecular Metabolism, 48, 101221.

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Fasted Serum Biomarker Analysis

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Fasted serum was used to measure serum lactate (K607; Biovision), ALT (TR71121; Thermo Scientific), AST (TR70121; Thermo Scientific), beta-hydroxybutyrate (Biovision K632), and serum-free fatty acids (#sfa-1; Zen-Bio).

Masschelin P.M., Saha P., Ochsner S.A., Cox A.R., Kim K.H., Felix J.B., Sharp R., Li X., Tan L., Park J.H., Wang L., Putluri V., Lorenzi P.L., Nuotio-Antar A.M., Sun Z., Kaipparettu B.A., Putluri N., Moore D.D., Summers S.A., McKenna N.J, & Hartig S.M. (2023). Vitamin B2 enables regulation of fasting glucose availability. eLife, 12, e84077.

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Quantifying Metabolic Biomarkers in Serum

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Serum Free Fatty Acid Quantification

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One hundred to 300 ul of blood was collected into 1.5 mL tubes from adult mice by submandibular bleeding. This blood was left to clot overnight at 4°C, and then spun at 6000 RPM for 20 min at 4°C in order to separate the clot from the serum. 5 ul of each serum sample was used to detect the total levels of free fatty acids using a serum/plasma fatty acid detection kit (Zenbio, Cat # SFA-1). In order to control for any hemolysis that could affect the assay by altering the color of the serum, 5 ul of each sample was diluted up to the total volume required for the assay using the supplied dilution buffer. The optical density of each sample in dilution buffer was subtracted from the optical density obtained from running the assay.

Fricano C.J., DeSpenza T J.r., Frazel P.W., Li M., O'Malley A.J., Westbrook G.L, & Luikart B.W. (2014). Fatty acids increase neuronal hypertrophy of Pten knockdown neurons. Frontiers in Molecular Neuroscience, 7, 30.

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