The human liver tumor cell line MHCC97-H was obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China) and maintained in Dulbecco's modified Eagle's medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS; Excell Biology, Inc., Shanghai, China), penicillin (100 U/ml) and streptomycin (100 µg/ml) (Gibco; Thermo Fisher Scientific, Inc.) under 95% air and 5% CO2 at 37°C. For cell treatments, MHCC97-H cells were cultured and treated with FK866 (Beyotime Institute of Biotechnology; Shanghai, China) at concentrations of 0, 1.25, 2.5, 5, 10, 20 and 40 nM.
Mhcc97h
Manufactured by Beyotime
Sourced in China
The MHCC97H is a laboratory equipment designed for cell culture applications. It serves as an incubator for maintaining optimal environmental conditions, such as temperature and humidity, to support the growth and maintenance of cell lines in a controlled setting. The device's core function is to provide a stable and regulated environment for cell cultivation and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fk866, by Beyotime (1 mentions)
Mhcc97h, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
Thle 3, by American Type Culture Collection (1 mentions)
Thle 3 cells, by American Type Culture Collection (1 mentions)
Huh7 cells, by Procell (1 mentions)
Hcclm3, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Plasmid overexpressing hsa circ 0003239, by GenePharma (1 mentions)
5 protocols using mhcc97h
1
Culturing MHCC97-H Liver Tumor Cells
2
Culturing Human Liver Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HCC cell lines HepG2, HuH7, Li7, SNU387, and SNU182 were kindly provided by the Stem Cell Bank, Chinese Academy of Sciences, and MHCC97H cells were obtained from Beyotime Company (Shanghai, China). The human liver cells THLE3 were obtained from ATCC. HepG2, HuH7, and MHCC97H cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Li7, SNU387, and SNU182 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin. Then, THLE3 cells were cultured in Endothelial Cell Medium (ECM) supplemented with 5% FBS, 1% endothelial cell growth supplement (ECGS), and 1% penicillin/streptomycin. All cells were maintained in a humidified atmosphere containing 5% CO2 at 37°C.
3
Hepatocellular Carcinoma Cell Lines Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
THLE-3 cells were purchased from the American Type Culture Collection (Rockville, MD, USA). Huh7 cells were obtained from Procell Life Science&Technology Co., Ltd. (Wuhan, China). The Hep-3B, HCCLM3 and MHCC97H cell lines were purchased from Beyotime Biotechnology (Shanghai, China). THLE-3 and Hep-3B cells were cultured in MEM (#E600020), while Huh7, HCCLM3, and MHCC97H cells were cultured in DMEM (#E600004). All media were replenished with 10% fetal bovine serum (FBS, #E600001) and 1 × penicillin–streptomycin solution (#E607011) before use, and were refreshed every 3 days. Cells were cultured under 5% CO2 at 37°C. The reagents used in the cell culture were obtained from the BBI Life Sciences Corporation (Shanghai, China).
4
Establishment of Luciferase-Expressing Liver Cancer Cell Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An MHCC97-H hepatic carcinoma cell line that could be used for screening and identification, and could stably express luciferase, was established by Beyotime Biotechnology Co. Ltd. After amplification and purification of the luciferase gene-carrying plasmids, they were effectively transfected into the highly metastatic human liver cancer cell line MHCC97-H. The cell line was screened for high positive expression. When the MHCC97-H cells had been cultured to the logarithmic growth phase, we used trypsin for digestion, and resuspended them in a cell culture medium to prepare a single-cell suspension. We counted the cells to ensure the live cell ratio was greater than 98%, recentrifuged the cell suspension (1000 r/min for 3 min), resuspended it in an appropriate amount of saline, and injected 5 x 10 6 cells/0.2 mL into the liver of each nude mouse to prepare the models.
5
Culturing and Transfecting Liver Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The liver cancer cell lines HepG2, Hep3B, Huh7, and SNU-387 were obtained from the Cell Bank, Type Culture Collection, Chinese Academy of Science. MHCC97H cells were purchased from Beyotime Biotechnology. Cells were cultured in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and were incubated at 37 °C with 5% CO 2 .
Plasmid overexpressing hsa_circ_0003239 and small interfering RNAs (siRNAs) targeting hsa_circ_0001394 were purchased from GenePharma Biotechnology. siR-NAs against hsa_circ_0002003 were designed and synthesized by Guangzhou Geneseed Biotech Co., Ltd. siRNA sequences are shown in Table S1. Cells were transfected with siRNAs and plasmids using Lipofectamine 3000 Transfection Reagent (Invitrogen) according to the manufacturer's instructions. All experiments were performed with mycoplasma-free cells.
Plasmid overexpressing hsa_circ_0003239 and small interfering RNAs (siRNAs) targeting hsa_circ_0001394 were purchased from GenePharma Biotechnology. siR-NAs against hsa_circ_0002003 were designed and synthesized by Guangzhou Geneseed Biotech Co., Ltd. siRNA sequences are shown in Table S1. Cells were transfected with siRNAs and plasmids using Lipofectamine 3000 Transfection Reagent (Invitrogen) according to the manufacturer's instructions. All experiments were performed with mycoplasma-free cells.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!