The proliferation of specific cells was assessed by the Cell Counting Kit-8 (CCK-8) assay kit (Invitrogen, Shanghai, China). Approximately 104 cells were seeded in each well of a 96-well plate, and 10 μL CCK-8 was added to 90 μL culture medium. After incubation at 37 °C for 2.5 h, the absorbance was detected at 450 nm and the OD450 value is correlated with live cell numbers.
Cell counting kit 8 cck 8 assay kit
Manufactured by Thermo Fisher Scientific
The Cell Counting Kit-8 (CCK-8) assay kit is a colorimetric method for determining the number of viable cells in cell proliferation and cytotoxicity assays. The kit uses a water-soluble tetrazolium salt to measure the activity of dehydrogenases in living cells, which directly correlates with the number of viable cells.
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2 protocols using cell counting kit 8 cck 8 assay kit
1
Cell Proliferation Quantification by CCK-8 Assay
2
Evaluating MCF-7 Cell Proliferation
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The proliferation of MCF-7 cell was assessed by the Cell Counting Kit-8 (CCK-8) assay kit (Invitrogen). Approximately 1 × 10 4 cells were seeded in each well of 96-well plates, and 10 µL of CCK-8 was added to 90 µL of culture medium. After incubation at 37°C for 2.5 h, the absorbance was detected at 450 nm, and the OD450 value was correlated with live cell numbers.
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