Ftir 300e infrared spectrophotometer

The ¹H, ¹³C and 2D NMR spectra were recorded at 400 and 100 MHz, respectively, using TMS as internal standard in methanol-d₄, using the residual solvent peak (δ_H= 3.34 and δ_C= 49.9) as references, on Bruker Avance III 400 MHz with BBFO Smart Probe and Bruker 400 MHz AEON Nitrogen-Free Magnet (Bruker AG, Switzerland). Carbon multiplicities were determined using DEPT-Q experiments. The optical rotation values were determined using a Jasco P-1020 polarimeter (Easton, MD, USA). The UV spectrum in methanol was obtained using a Shimadzu UV 2401PC spectrophotometer. The IR spectra were obtained using a Jasco, FT IR 300E infrared spectrophotometer. ESI-MS data were obtained using a Thermo Scientific LTQ/XL Orbitrap (Waltham, MA USA). Column chromatography was performed using silica gel 60 (63–200 µm, Fluka, Sigma-Alderich, Germany); Polyamide-6 (50–160 µm, Sigma-Aldrich, Germany) and Sephadex LH-20 (Sigma-Aldrich, Germany). HPLC separations were conducted using an Agilent 1260 Infinity preparative pump (G1361A), Agilent 1260 Diode array detector VL (G1315 D), Agilent 1260 Infinity Thermostand column compartment (G1361 A) and Agilent 1260 Infinity preparative Autosampler (G2260A). YMC-Pack ODS-A A-324 column (i.d. 10 × 300 mm, YMC, Kyoto, Japan).