Evo 18 special edition sem

The morphology of breast carcinoma cells under different conditions were observed under scanning electron microscope (SEM). By following the protocol of Bhattacharya et al. (2015) (link) the cells were grown on poly-L-Lysine (1:10) coated glass slide (1 cm × 1 cm) overnight at 37°C in 5% CO₂. Next day, cells were treated with specific dose of Phemindole and kept in the CO₂ incubator for overnight. Next day, the cells were washed by sodium cacodylate buffer, and then fixed with 2.5% glutaraldehyde buffered in sodium cacodylate for 1 h. The samples were washed with sodium cacodylate buffer thrice, and dehydrated through a series of alcohol concentrations (10, 30, 50, 70, 90, and 100%), and subjected to air drying. The samples were then visualized by EVO-18 special edition SEM (ZEISS, Germany).

Early log phase cultures of S. aureus and S. enterica were subjected to different concentration of peptides and incubated at 37°C. The bacterial pellets after centrifugation (5000 g for 10 min) were washed with 0.1M cacodylate buffer (pH 7.2) and were fixed in 2.5% gluteraldehyde at 4°C for 2 h. For scanning electron microscopy, bacteria were dehydrated using gradient series of ethanol wash (30, 50, 70, 90 and absolute). The samples were air-dried overnight, gold coated (Eiko IB-3 ion coater), and viewed in a Zeiss EVO 18 Special Edition SEM. For transmission electron microscopy, fixed samples were adhered to hexagonal PELCO® Grids (Ted Pella, Inc. USA), washed once with Mili-Q water and stained with uranyl acetate (2%) buffer. Samples were viewed in a JEM 1011 (Jeol, Japan) microscope.