Y-27632 and 4’,6-diamidino-2-phenylindol (DAPI) were purchased from Wako Pure Chemical Industries (Osaka, Japan) and Polysciences (Warrington, PA), respectively. Matrigel (growth factor-reduced) was purchased from Corning Incorporated (Corning, NY). Rabbit polyclonal antibodies against human Solo were prepared as previously described [14 (link)]. Other antibodies were purchased as follows: anti-FLAG (M2; Sigma-Aldrich, St. Louis, MO), anti-GFP (A6455; Life Technologies, Camarillo, CA), anti-β-actin (AC-15; Sigma-Aldrich), anti-K18 (DA-7; BioLegend, San Diego, CA), anti-GM130 (CSB-PA600856ESR1HU; Flarebio Biotech LLC, College Park, MD), anti-β4 (58XB4; BioLegend) for immunofluorescence, and anti-β4 (Clone 7; BD Biosciences, Franklin Lakes, NJ) for immunoblotting. Secondary antibodies, anti-mouse IgG-Alexa 488, anti-mouse IgG-Alexa 568, anti-rabbit IgG-Alexa 568, and anti-rat IgG-FITC, were purchased from Thermo Fisher Scientific (Waltham, MA).
Anti k18 da 7
Manufactured by BioLegend
Anti-K18 (DA-7) is a monoclonal antibody that recognizes the K18 protein. K18 is a type I intermediate filament protein that is expressed in simple and stratified epithelial cells. The antibody can be used to detect K18 in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti gfp a6455, by Thermo Fisher Scientific (2 mentions)
Anti β actin ac 15, by Merck Group (2 mentions)
Anti flag m2, by Merck Group (2 mentions)
Anti mouse igg alexa 488, by Thermo Fisher Scientific (1 mentions)
Y 27632, by Fujifilm (1 mentions)
Anti β4 clone 7, by BD (1 mentions)
Alexa 568 anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Anti rat igg fitc, by Thermo Fisher Scientific (1 mentions)
Matrigel, by Corning (1 mentions)
Rhodamine labeled phalloidin, by Fujifilm (1 mentions)
Anti rhoa sc 418, by Santa Cruz Biotechnology (1 mentions)
Anti k18 ks 18.04, by Progen Biotechnik (1 mentions)
Flag peptide, by Merck Group (1 mentions)
Anti β catenin, by BD (1 mentions)
2 protocols using anti k18 da 7
1
Antibody Reagents for Cell Biology
2
Assay for Cytoskeleton and Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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FLAG peptide was purchased from Sigma-Aldrich (St. Louis, MO), recombinant K8 and K18 proteins were purchased from Progen (Darra, Australia), rhodamine-labeled phalloidin was purchased from Wako Pure Chemical Industries (Osaka, Japan), Alexa Fluor 568–labeled phalloidin was purchased from Life Technologies (Grand Island, NY), LPA was purchased from Cayman Chemical (Ann Arbor, MI), and Cytopainter MitoRed Indicator was purchased from Abcam (Cambridge, United Kingdom). Rabbit polyclonal antibodies against human Solo, which also recognized dog Solo, were prepared as previously described (Abiko et al., 2015 (link)). Other antibodies were purchased as follows: anti-K18 (DA-7; BioLegend, San Diego, CA) for HeLa cells, anti-K18 (Ks 18.04; Progen, Heidelberg, Deutschland) for MDCK cells, anti-FLAG (M2; Sigma-Aldrich), anti-GFP (A-6455; Life Technologies, Camarillo, CA), anti–β-actin (AC-15; Sigma-Aldrich), anti–β-catenin (Clone 14; BD Biosciences, Franklin Lakes, NJ), anti-plakoglobin (Clone 15; BD Biosciences), and anti-RhoA (sc-418; Santa Cruz Biotechnology, Dallas, TX).
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