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Cd49d l25

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CD49d (L25) is a monoclonal antibody that recognizes the alpha 4 subunit (CD49d) of the VLA-4 integrin. It is used in flow cytometry applications for the identification and enumeration of cells expressing CD49d.

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Lab products found in correlation

T bet, by BioLegend (4 mentions) Staphylococcal enterotoxin b, by Merck Group (4 mentions) Eomesodermin wd1928 pe efluor610, by Thermo Fisher Scientific (4 mentions) Cd107a, by BD (2 mentions) Pepmix hiv nef ultra, by JPT Peptide Technologies (2 mentions) Cd4 t4d11 ecd, by Beckman Coulter (2 mentions) T bet 4b10 ax647 unlabeled cd28 l293, by BD (2 mentions) Unlabeled functional grade cd3 okt3, by Thermo Fisher Scientific (2 mentions) Perforin b d48 pe, by Cell Sciences (2 mentions) Unlabeled pan anti tgf β cat no t9429, by Merck Group (2 mentions) Rabbit igg cat no i5006, by Merck Group (2 mentions) Cd45ro, by BioLegend (2 mentions) Cd103, by BD (2 mentions) Unlabeled cd28 l293, by BD (2 mentions) S1pr1 sw4gypp efluor660, by Thermo Fisher Scientific (2 mentions) Mip 1β, by BD (2 mentions) Tnf α, by BioLegend (2 mentions) Cd107a, by BioLegend (2 mentions) Interleukin 2 (il 2), by BioLegend (2 mentions) Ifn γ, by BD (2 mentions)

5 protocols using cd49d l25

1

Multiparameter Flow Cytometry Panel

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The following fluorochrome-labeled monoclonal antibodies were used in flow cytometry: CD107a (H4A3: PE-Cy5, PE-Cy7), CD8 (SK1: APC-H7, FITC), CCR7 (3D12: PE-Cy7), Granzyme B (BG11: V450) from BD Biosciences (San Jose, CA); CD4 (T4D11: ECD) from Beckman Coulter (Brea, CA); CD4 (RPA-TA: BV785), CD45RO (UCHL1: BV785), CD27 (O323: BV650), Granzyme A (CB9: Ax647), CD3 (UCHT-1: Ax700), and T-bet (4B10: BV711) from Biolegend (San Diego, CA). CD3 (S4.1: Qdot655) was purchased from Invitrogen (Carlsbad, CA). T-bet (4B10: Ax647); unlabeled CD28 (L293), and CD49d (L25) were from BD Pharmingen (San Diego, CA). Eomesodermin (WD1928: PE-eFluor610) and unlabeled functional grade CD3 (OKT3) were from eBiosciences (San Diego, CA). Perforin (B-D48: PE) was from Cell Sciences (Canton, MA). Unlabeled pan anti-TGF-β (Cat No. T9429) and rabbit IgG (Cat No. I5006) were from Sigma Aldrich. The HIV Gag peptide pool (p55, HXB2 sequence) consisted of 15-mers with an 11 amino acid overlap (BD Biosciences). HIV Nef peptide pool (PepMix™ HIV NEF Ultra, JPT Peptide Technologies, Berlin, Germany) consisted of 150 15-mers with an 11 amino acid overlap. Staphylococcal enterotoxin B (SEB) was from Sigma Aldrich.
Kiniry B.E., Ganesh A., Critchfield J.W., Hunt P.W., Hecht F.M., Somsouk M., Deeks S.G, & Shacklett B.L. (2016). Predominance of Weakly Cytotoxic, T-betLowEomesNeg CD8+ T-cells in Human Gastrointestinal Mucosa: Implications for HIV Infection. Mucosal immunology, 10(4), 1008-1020.
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2

Multiparametric Flow Cytometry Assay

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The following monoclonal antibodies were used in flow cytometry: CD8 (SK1: APC-H7), CCR7 (3D12: PE-Cy7), CD69 (L78: PE), IFN-γ (B27: PE-Cy7), MIP-1β (D21-1351: PerCP-Cy5.5), from BD Biosciences (San Jose, CA); CD103 (Ber-ACT8: Ax488), TNF-α (MAb11: BV605), IL-2 (MQ1-17H12: BV650), CD107a (H4A3: BV711), CD4 (RPA-TA: BV570, BV605), CD45RO (UCHL1: BV785), CD27 (O323: BV650), CD3 (UCHT-1: Ax700), and T-bet (4B10: BV711) from Biolegend (San Diego, CA). Unlabeled CD28 (L293), and CD49d (L25) were from BD Pharmingen (San Diego, CA). Eomesodermin (WD1928: PE-eFluor610) and S1PR1 (SW4GYPP: eFluor660) were from ThermoFisher (Waltham, MA). The HIV-1 Gag peptide pool (p55, HXB2 sequence) consisted of 15-mers with an 11 amino acid overlap (BD Biosciences). Staphylococcal enterotoxin B (SEB) was from Sigma Aldrich.
Kiniry B.E., Li S., Ganesh A., Hunt P.W., Somsouk M., Skinner P.J., Deeks S.G, & Shacklett B.L. (2017). Detection of HIV-1-Specific Gastrointestinal Tissue Resident CD8+ T-cells in Chronic Infection. Mucosal immunology, 11(3), 909-920.
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3

Comprehensive T Cell Characterization

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The following antibodies were used for flow cytometry analysis: CD3 (SK7; BD), CD4 (SK3; eBioscience), CD8 (RPA-T8; BD), CD69 (FN50; BioLegend), TNF (MAb11; eBioscience), IFN-γ (4S.B3; eBioscience), and MHC I (W6/32; Bio X Cell). The following functional grade antibodies were used to stimulate T cells in culture: CD28 (L293; BD), CD49d (L25; BD), and CD3 (SK7; eBioscience). The pan–anti–MHC II–blocking antibody Tu39 and isotype control IgG2a κ were from BioLegend. The anti–HLA-DR–blocking antibody G46-6 was from BD. The 15mer-overlapping peptide pool covering Ad169 IE-1 and custom-made 15mer-overlapping peptide pools covering the Towne and Toledo IE-1 sequences were from JPT Peptide Technologies. Individual peptides used for truncation studies and for pulsing HLA transfectants were synthesized by JPT or Genemed Synthesis Inc. HLA-B08 tetramers folded with the EM9 and QV9 peptides were from the National Institutes of Health tetramer core facility. Recombinant human IL-2, IL-4, and GM-CSF were from Immunex.
Murray S.E., Nesterenko P.A., Vanarsdall A.L., Munks M.W., Smart S.M., Veziroglu E.M., Sagario L.C., Lee R., Claas F.H., Doxiadis I.I., McVoy M.A., Adler S.P, & Hill A.B. (2017). Fibroblast-adapted human CMV vaccines elicit predominantly conventional CD8 T cell responses in humans. The Journal of Experimental Medicine, 214(7), 1889-1899.
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4

Multiparametric Flow Cytometry Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following monoclonal antibodies were used in flow cytometry: CD8 (SK1: APC-H7), CCR7 (3D12: PE-Cy7), CD69 (L78: PE), IFN-γ (B27: PE-Cy7), MIP-1β (D21-1351: PerCP-Cy5.5), from BD Biosciences (San Jose, CA); CD103 (Ber-ACT8: Ax488), TNF-α (MAb11: BV605), IL-2 (MQ1-17H12: BV650), CD107a (H4A3: BV711), CD4 (RPA-TA: BV570, BV605), CD45RO (UCHL1: BV785), CD27 (O323: BV650), CD3 (UCHT-1: Ax700), and T-bet (4B10: BV711) from Biolegend (San Diego, CA). Unlabeled CD28 (L293), and CD49d (L25) were from BD Pharmingen (San Diego, CA). Eomesodermin (WD1928: PE-eFluor610) and S1PR1 (SW4GYPP: eFluor660) were from ThermoFisher (Waltham, MA). The HIV-1 Gag peptide pool (p55, HXB2 sequence) consisted of 15-mers with an 11 amino acid overlap (BD Biosciences). Staphylococcal enterotoxin B (SEB) was from Sigma Aldrich.
Kiniry B.E., Li S., Ganesh A., Hunt P.W., Somsouk M., Skinner P.J., Deeks S.G, & Shacklett B.L. (2017). Detection of HIV-1-Specific Gastrointestinal Tissue Resident CD8+ T-cells in Chronic Infection. Mucosal immunology, 11(3), 909-920.
+ Open protocol
+ Expand
5

Multiparameter Flow Cytometry Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following fluorochrome-labeled monoclonal antibodies were used in flow cytometry: CD107a (H4A3: PE-Cy5, PE-Cy7), CD8 (SK1: APC-H7, FITC), CCR7 (3D12: PE-Cy7), Granzyme B (BG11: V450) from BD Biosciences (San Jose, CA); CD4 (T4D11: ECD) from Beckman Coulter (Brea, CA); CD4 (RPA-TA: BV785), CD45RO (UCHL1: BV785), CD27 (O323: BV650), Granzyme A (CB9: Ax647), CD3 (UCHT-1: Ax700), and T-bet (4B10: BV711) from Biolegend (San Diego, CA). CD3 (S4.1: Qdot655) was purchased from Invitrogen (Carlsbad, CA). T-bet (4B10: Ax647); unlabeled CD28 (L293), and CD49d (L25) were from BD Pharmingen (San Diego, CA). Eomesodermin (WD1928: PE-eFluor610) and unlabeled functional grade CD3 (OKT3) were from eBiosciences (San Diego, CA). Perforin (B-D48: PE) was from Cell Sciences (Canton, MA). Unlabeled pan anti-TGF-β (Cat No. T9429) and rabbit IgG (Cat No. I5006) were from Sigma Aldrich. The HIV Gag peptide pool (p55, HXB2 sequence) consisted of 15-mers with an 11 amino acid overlap (BD Biosciences). HIV Nef peptide pool (PepMix™ HIV NEF Ultra, JPT Peptide Technologies, Berlin, Germany) consisted of 150 15-mers with an 11 amino acid overlap. Staphylococcal enterotoxin B (SEB) was from Sigma Aldrich.
Kiniry B.E., Ganesh A., Critchfield J.W., Hunt P.W., Hecht F.M., Somsouk M., Deeks S.G, & Shacklett B.L. (2016). Predominance of Weakly Cytotoxic, T-betLowEomesNeg CD8+ T-cells in Human Gastrointestinal Mucosa: Implications for HIV Infection. Mucosal immunology, 10(4), 1008-1020.
+ Open protocol
+ Expand

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