Mc903

Manufactured by Merck Group

Sourced in United States

The MC903 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory use, but its core function is to provide precise temperature control and monitoring capabilities. The MC903 can be used to maintain specific temperatures for a variety of applications, such as incubation, sample preparation, and other temperature-sensitive processes. No further details or interpretations on the intended use of this product are provided.

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Lab products found in correlation

25 protocols using mc903

Epicutaneous OVA Sensitization in C57/B6 Mice

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C57/ B6 mice were obtained from the Experimental Animal Center in Guangdong. C57/ B6 mice were epicutaneously sensitized twice and challenged with OVA as previous description (10) . In brief , OVA solution (100 μg OVA / 100 μl PBS) was placed on a patch of 1 cm 2 filter paper , pasted to the depilated skin and fixed with gaze and bandage for 1 week. All of the mice had three-1-week exposure separated by 2-week break from each other. At the beginning, mice (6-8 per group) were between the ages of 8 and 9 weeks. Mice were killed at the end of the third challenge. For MC903 treatment as previous description, MC903 (Sigma, USA) dissolved in ethanol was topically applied on each ear (1.125nmol MC903 / 25 μL ) of 6-to 8-week-old female C57/B6 mice every day. 25μL ethanol was applied on ear of mice as vehicle control (11) . All procedures were approved by the Animal Use and Care Committee of Peking University Shenzhen Hospital.

Jia H.Z., Liu S.L., Zou Y.F., Chen X.F., Yu L., Wan J., Zhang H.Y., Chen Q., Xiong Y., Yu B, & Zhang W. (2018). MicroRNA-223 is involved in the pathogenesis of atopic dermatitis by affecting histamine-N-methyltransferase. Cellular and molecular biology (Noisy-le-Grand, France), 64(3).

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Murine Models of Atopic Dermatitis

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For the calcipotriol (MC903)-induced AD mouse model, 1 nmol of MC903 (Sigma-Aldrich) dissolved in 10 μl of ethanol was painted on the ears of 8-week-old mice (C57BL/6 background) for 15 d consecutively. Photographs of the ears were taken every day and the ear thickness was measured with vernier calipers (Meinaite) every day. For the OVA-induced AD mouse model, 8-week-old mice were intraperitoneally inoculated with 10 μg of chicken OVA mixed with 4 mg of aluminum hydroxide (ImjectAlum; Thermo Fisher Scientific) in a volume of 200 μl at 1-week intervals (that is, at days 0, 7 and 14). At day 13, the dorsal skin of mice was shaved and tape stripped six times with 3M tape. At day 14, 100 μg of OVA in 100 μl of PBS was placed on a 1-cm² patch of sterile gauze to make an OVA patch, which was attached on the shaved dorsal skin with a transparent dressing (Tegaderm, 3M) for 7 d (that is, from day 14 to day 20). OVA patches were changed daily. Each mouse had a total of three 1-week exposures to the patch at the same site of separation from each other at 2-week intervals. Dorsal skin or ears were collected for flow cytometry, immunoblotting, RT–qPCR, ELISA or immunofluorescence and hematoxylin and eosin (H&E) staining.

Ni X., Xu Y., Wang W., Kong B., Ouyang J., Chen J., Yan M., Wu Y., Chen Q., Wang X., Li H., Gao X., Guo H., Cui L., Chen Z., Shi Y., Zhu R., Li W., Shi T., Wang L.F., Huang J., Dong C, & Lai Y. (2022). IL-17D-induced inhibition of DDX5 expression in keratinocytes amplifies IL-36R-mediated skin inflammation. Nature Immunology, 23(11), 1577-1587.

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Topical MC903 Induces TSLP Expression

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To induce TSLP expression in the keratinocytes, we used vitamin D analogue MC903 (calcipotriol, Sigma ).^{35 (link), 36 (link)} MC903 was dissolved in 100% ethanol and topically applied on mouse ears (2 nmol in 25 μl per ear) for 7 days, the same amount of ethanol was used as vehicle control.

Wang Q., Du J., Zhu J., Yang X, & Zhou B. (2014). TSLP Signaling in CD4+ T cells is Required for Th2 Memory. The Journal of allergy and clinical immunology, 135(3), 781-791.e3.

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Modeling Atopic Dermatitis Itch Behavior

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To establish a prolonged MC903‐induced model of AD, MC903 (2 nmol/20 μl in ethanol, Sigma Aldrich) was applied topically to the left ear of C57BL/6 mice. This was repeated daily for 7 consecutive days, resulting in the induction of an AD‐like chronic itch model.²⁵To analyze the influence of IL‐20 on IL‐13‐induced itch‐like behavior, C57 BL/6 mice (n = 8 mice/group) received a left cheek injection of vehicle, IL‐20 (2.5 μg/10 μl) or IL‐13 (1 μg/10 μl), or a co‐injection of IL‐20 (2.5 μg/10 μl) and IL‐13 (1 μg/10 μl). Bouts of hind limb scratches were video recorded for 1 h and scratching bouts directed to the injection site were considered indicative of pruritus. All the mice were video‐recorded for 1 h for the analysis of scratching bouts. Data presented as means ± SEMs (n = 8 mice/group); *p < .05, **p < .01, and ***p < .001, 2‐way analysis of variance (ANOVA).

Lu Z., Xiao S., Chen W., Zhu R., Yang H., Steinhoff M., Li Y., Cheng W., Yan X., Li L., Xue S., Larkin C., Zhang W., Fan Q., Wang R., Wang J, & Meng J. (2022). IL‐20 promotes cutaneous inflammation and peripheral itch sensation in atopic dermatitis. The FASEB Journal, 36(6), e22334.

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Topical MC903 Induces Atopic Dermatitis

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An AD mouse model was established by topically utilizing a low-calcemic analogue calcipotriol of vitamin D3, MC903 (Sigma-Aldrich, St. Louis, MO, USA), as per a reported protocol.36 (link) In short, MC903 (2 nmol, 20 μL) dissolved in 95% ethanol was applied topically to the dorsal side of the left ear. On the 7th day, an MC903-induced AD model was successfully established. Subsequently, MC903 was still administered to mice in the AD group, the AD + CQ group, the AD + CQ + Nigericin group, and the AD + CQ + poly (I:C) group.37 (link) The Control group was given an equal amount of 95% ethanol.

Wei M., Yang H., Shao Z., Wan H., Wang Y, & Chen W. (2024). Effect of Chloroquine on Type 2 Inflammatory Response in MC903-Induced Atopic Dermatitis Mice. Clinical, Cosmetic and Investigational Dermatology, 17, 1093-1105.

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Wound Healing in Atopic Dermatitis Mouse Models

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Female BALB/C and C57BL/6 mice were purchased from Harlan Bioscience (Indianapolis, Ind). The generation of Stat6VT transgenic mice was previously described ^{17 (link)}. These mice express the human STAT6 gene with V547 and T548 mutated to alanine under transcriptional control of the CD2 locus control region. IL-4–deficient mice (Il4^−/−) were purchased from The Jackson Laboratory (Bar Harbor, ME) and mated to Stat6VT transgenic mice. Stat6^−/− mice were generated and backcrossed for at least 10 generations to BALB/c mice as described previously ^{18 (link)}. Mouse ears were punched using a 2 mm punch (Kent Scientific) to determine the in vivo wound healing response. In some experiments, mice were treated for two days with ointment containing BSA (Promega) or fibronectin purified from human plasma (Sigma). To determine wounding response in an induced model of AD-like disease, WT and Stat6^−/− mice were treated with the vitamin D analogue MC903 (Calcipotriol, Sigma) as previously described ^{19 (link)}. MC903 was dissolved in 100% ethanol and topically applied on mouse ears (2 nmol in 25 μL per ear) for 5 days. Ethanol alone was used as a vehicle control. Ears were punched at day 6 after treatment. Mice were maintained in pathogen-free conditions, and all studies were approved by the Animal Care and Use Committee of the Indiana University School of Medicine.

Serezani A.P., Bozdogan G., Sehra S., Walsh D., Krishnamurthy P., Potchanant E.A., Nalepa G., Goenka S., Turner M.J., Spandau D.F, & Kaplan M.H. (2016). IL-4 impairs wound healing potential in the skin by repressing fibronectin expression. The Journal of allergy and clinical immunology, 139(1), 142-151.e5.

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Establishing AD Mouse Model Using MC903

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The AD mouse model was established using MC903 stimulation for 16 days. A total of 2 nmol of MC903 (Sigma-Aldrich Corp, St. Louis, MO, United States) was topically applied in 5 μL of ethanol to one ear of a mouse every other day for 16 days. Ear thickness was measured with a dial thickness gauge (Model G, Peacock, Ozakimfg Co, Ltd, Tokyo, Japan) and snatching time within 5 min were recorded to assess itching severity every other day for 17 days, followed by sacrificing the mice for post mortem analysis of AD skin lesions. Quick and continuous multiple scratching within a very short period was considered as one-time scratching. All animal experiments were approved by the Animal Experimentation Ethics Committee of the Chinese University of Hong Kong, Hong Kong.

Hou T., Sun X., Zhu J., Hon K.L., Jiang P., Chu I.M., Tsang M.S., Lam C.W., Zeng H, & Wong C.K. (2020). IL-37 Ameliorating Allergic Inflammation in Atopic Dermatitis Through Regulating Microbiota and AMPK-mTOR Signaling Pathway-Modulated Autophagy Mechanism. Frontiers in Immunology, 11, 752.

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Atopic Dermatitis Induction and Behavioral Evaluation

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The right cheek was shaved two days before starting the experiments under brief anesthesia with isoflurane. To induce AD-like lesions, a vitamin D analog, MC903 (1.65 µg in 20 µl ethanol; Sigma-Aldrich, USA), was applied topically on the right cheek once a day for 7 days. Control mice were treated similarly with vehicle (ethanol). Lesion severity, scratching behavior, and anxiety- and depression-like behaviors were observed on days 10 and 11 after the first injection of MC903. A detailed schedule is shown in Fig. 6.
Fig. 6

Schematic overview for overall experimental design applied to each group of animals. NOR untreated control group, MC903 MC903-induced atopic dermatitis group, pAP MC903- and preventive acupuncture-treated group, tAP MC903- and therapeutic acupuncture-treated group, CP MC903- and acupuncture at control point (non-acupoint)-treated group

Yeom M., Ahn S., Jang S.Y., Jang J.H., Lee Y., Hahm D.H, & Park H.J. (2022). Acupuncture attenuates comorbid anxiety- and depressive-like behaviors of atopic dermatitis through modulating neuroadaptation in the brain reward circuit in mice. Biological Research, 55, 28.

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Topical Treatments for Skin Inflammation

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For inhibition of glucose transport, 1 mg/ml WZB-117 dissolved in acetone (Sigma-Aldrich) or vehicle (100% acetone) was applied topically once daily onto the ears of female ft/ft mice for 9 days according to previous work (Zhang et al., 2018 (link)). The epidermis was collected 6 hours after the last treatment. Psoriasis-like skin inflammation was induced in adult, age-matched, female C57BL/6 mice by topical application of a daily dose of 12.5 mg of Aldara cream (Meda Pharma GmbH, Frankfurt, Germany), containing 5% IMQ onto ears for 5 days (van der Fits et al., 2009 (link)). Control mice were topically treated with petroleum jelly. The epidermis was collected 24 hours after the last treatment. To induce ADL, 45 μM MC903 (Sigma Aldrich) in 12.5 μl ethanol was topically applied onto both sides of the ears of female C57BL/6 mice daily for 9 days consecutively (Moosbrugger-Martinz et al., 2017 (link)). Control mice were topically treated with ethanol (vehicle). The epidermis was collected 24 hours after the last treatment.

Pavel P., Leman G., Hermann M., Ploner C., Eichmann T.O., Minzaghi D., Radner F.P., Del Frari B., Gruber R, & Dubrac S. (2021). Peroxisomal Fatty Acid Oxidation and Glycolysis Are Triggered in Mouse Models of Lesional Atopic Dermatitis. JID Innovations, 1(3), 100033.

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Phorbol ester and Vitamin D analogue protocol

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Phorbol 12-myristate 13-acetate (PMA) and MC903 (calcipotriol) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phloxine O (CAS, 13473-26-2; IUPAC name, 2′,4′,5′,7′-tetrabromo-4,5,6,7-tetrachloro-3′,6′-dihydroxyspiro[2-benzofuran-3,9′-xanthene]-1-one; molecular formula, C₂₀H₄Br₄Cl₄O₅) was obtained from LG Household and Health Care Co (Daejeon, Korea).

Lee H.E., Yang G., Kim K.B., Lee B.M, & Lee J.Y. (2018). Phloxine O, a Cosmetic Colorant, Suppresses the Expression of Thymic Stromal Lymphopoietin and Acute Dermatitis Symptoms in Mice. Biomolecules & Therapeutics, 26(5), 481-486.

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