C r7a plus chromatopac

HPLC was performed at room temperature. Regarding the apparatus, a Shimadzu LC-6AD pump was used with a Shimadzu SPD-10AV VP UV-VIS detector set at a wavelength of 246 nm and Shimadzu C-R7A plus Chromatopac (Kyoto, Japan). A YMC Pack ODS-AM column (I.D. of 6 mm, length of 150 mm; YMC Co., Ltd., Kyoto, Japan) was used as the analytical column. A mixture of acetonitrile and 50 mM citrate buffer with pH adjusted to 4.1 with phosphoric acid (35:65, v/v) was used as the mobile phase. The flow rate was set at 1 mL/min with an injection volume of 20 μL. PD concentrations were assessed using an absolute calibration curve.

UV-VIS absorption was measured with a Beckman DU640 spectrophotometer (Beckman Coulter, Inc., Brea, CA, USA). HPLC was used to detect PD with the Shimadzu SPD-10AV VP UV-VIS detector set at a wavelength of 246 nm and Shimadzu C-R7A plus Chromatopac (Kyoto, Japan). The size and ζ-potentials of the particles produced were measured using an ELS-Z2 apparatus (Otsuka Electronic Co., Ltd., Osaka, Japan). ¹H-NMR spectra were measured with a JNM-ECA600 II spectrometer (JEOL, Tokyo, Japan).

HPLC was performed at room temperature (25 °C) using a Shimadzu LC-6AD pump with a Shimadzu SPD-10AV VP UV-VIS detector set at a wavelength of 246 nm and Shimadzu C-R7A plus Chromatopac (Kyoto, Japan). A YMC Pack ODS-AM column (I.D. of 6 mm, length of 150 mm; YMC Co., Ltd., Kyoto, Japan) was used as the analytical column. A mixture of acetonitrile and 50 mM citrate buffer with pH adjusted to 4.1 with phosphoric acid (35:65, v/v) was used as the mobile phase. The injection volume was 20 µL, and the flow rate was set at 1 mL/min. PD concentrations were assessed using an absolute calibration curve.