Mouse monoclonal anti β actin ac 15

Mouse monoclonal ALDH1A2 (G2), mouse monoclonal CD68 (KP-1), rabbit polyclonal CD163 (H-130), mouse monoclonal CD204/SR-A (E-5), goat polyclonal CD206 (C-20), and goat polyclonal GFAP (C-19) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Dallas, TX, USA). Mouse monoclonal anti-β-Actin (AC-15) was purchased from Sigma-Aldrich (Sigma-Aldrich, St Louis, MO, USA). Rabbit polyclonal anti-MMP-7 and rabbit monoclonal anti-CD163 (EPR19518) were purchased from Abcam (Abcam, Waltham, MA, USA). Mouse monoclonal CD163 (5C6-FAT) was purchased from Novus Biological (Novus Biological, Littleton, CO, USA). Mouse monoclonal anti-ALDH1A1 (1G6) was purchased from Abnova (Abnova, Walnut, CA, USA); mouse monoclonal anti-ALDH1A3 (OTI4B6) was purchased from Invitrogen (Invitrogen, Waltham, MA, USA).

The antibodies and dilutions used for western blots were as follows: rabbit polyclonal anti-FBXW7 (1:500) and anti-AURKA (1:2,000) from Novus; rabbit polyclonal anti-MCL1 (S-19, 1:1,000), anti-Cyclin B1 (1:1,000), anti-phospho-histone H3 (Ser10, 1:500) and mouse monoclonal anti-PTTG1 (1:1,000) from Santa Cruz; mouse monoclonal anti-β-actin (Ac15, 1:20,000) and rabbit polyclonal anti-c-Myc (1:1,000) from Sigma; rabbit polyclonal anti-cleaved caspase-9 (Asp 315, 1:500), anti-cleaved caspase-3 (Asp 175, 1:500) from Cell Signaling; rabbit polyclonal anti-BubR1 (1:3,000) from Bethyl; mouse monoclonal anti-PARP (1:500) from BD Bioscience; mouse monoclonal anti-Cyclin E (1:500) from Monosan; mouse monoclonal anti-PLK1 (1:10,000) from Millipore, and rat monoclonal anti-HA-peroxidase (50 mU/mL) from Roche. For immunohistochemical analyses, anti- FBXW7 (1:2,000), anti- Cyclin E (1:100), anti- MCL1 (1:1,250), anti-AURKA (1:700), and anti-PLK1 (1:1,000) were used.