DTX was purchased from Knowshine (Shanghai, China). Methoxy poly(ethyleneglycol)-poly(ε-caprolactone) (MPEG-PCL)(Mw: 3 k–15 k) and FITC-conjugated poly(ethyleneglycol)-poly(ε-caprolactone) (FITC-PEG-PCL)(Mw: 3 k–15 k) were synthesized as previously described27 (link). The near infrared dye 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine Iodide (DiR) was purchased from Biotium (Hayward, CA). DAPI was purchased from Beyotime (Haimen, China).U87 and A549 cells were purchased from the Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). Plastic cell culture dishes and plates were purchased from Wuxi NEST Biotechnology Co. Ltd. (Wuxi, China). LysoTracker Red, MitoTracker Red, Click iT™ EdU flow cytometry assay kit, Dulbecco's Modified Eagle Medium (high glucose) cell culture medium (DMEM) and FBS were purchased from Life Technologies (Grand Island, NY, USA). All other chemicals were analytical reagent grades and were purchased from Sinopharm Chemical Reagent (Shanghai, China).
BALB/c nude mice (male, 4–5 weeks, 18–22 g) were obtained from the Shanghai Slac Laboratory Animal Co, Ltd. (Shanghai, China) and maintained under standard housing conditions. All animal experiments were carried out in accordance with protocols evaluated and approved by the ethics committee of SIchuan University.
BALB/c nude mice (male, 4–5 weeks, 18–22 g) were obtained from the Shanghai Slac Laboratory Animal Co, Ltd. (Shanghai, China) and maintained under standard housing conditions. All animal experiments were carried out in accordance with protocols evaluated and approved by the ethics committee of SIchuan University.