Bsm 33218 m

Manufactured by Bioss Antibodies

The Bsm-33218 M is a laboratory instrument designed for general analytical purposes. It provides precise measurement and data analysis capabilities for various applications within a research or testing environment. The core function of this equipment is to facilitate accurate data collection and processing, supporting the needs of scientific research and investigation.

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Lab products found in correlation

Stat3, by Bioss Antibodies (1 mentions) Integrin αvβ3 bs1310r, by Bioss Antibodies (1 mentions) Cxcr4 d1s7w, by Cell Signaling Technology (1 mentions) Phospho vegfr2 bs 2674r, by Bioss Antibodies (1 mentions) P fak, by Bioss Antibodies (1 mentions) Bs 23003r, by Bioss Antibodies (1 mentions) Pstat3, by Bioss Antibodies (1 mentions) Bs 1658r, by Bioss Antibodies (1 mentions) Bs 0519r, by Bioss Antibodies (1 mentions) P erk1 2 d13.14.4e, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) β actin, by Beyotime (1 mentions) Protease inhibitor, by Roche (1 mentions) Phosphatase inhibitor, by Roche (1 mentions) Mb001, by Bioworld Technology (1 mentions) Bs61583, by Bioworld Technology (1 mentions) Bs70205, by Bioworld Technology (1 mentions)

2 protocols using bsm 33218 m

Quantification of Signaling Pathway Proteins

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MSCs or ECs were lysed using ice-cold lysis buffer containing 1% protease inhibitors and phosphatase inhibitors (Roche Applied Science, Penzberg, Germany). Proteins were fractionated by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis and then transferred to polyvinylidene difluoride (PVDF) membranes. Protein expression was detected by incubation with antibodies against CXCR4 (D1S7W; Cell Signaling Technology), phospho-VEGFR2 (bs-2674R; Bioss), integrin αvβ3 (bs-1310R; Bioss), p-FAK (bsm-52155R; Bioss), FAK (bs-20735R; Bioss), p-JAK2 (SY24-03; Novus), JAK2 (bs-23003R; Bioss), p-STAT3 (bs-1658R; Bioss), STAT3 (bsm-33218 M; Bioss), ATF3 (bs-0519R; Bioss), CHOP (bs-20669R; Bioss), p-ERK1/2 (D13.14.4E; Cell Signaling Technology), ERK1/2 (137F5; Cell Signaling Technology) or β-actin (AF0003; Beyotime).

Tang Y., Luo K., Chen Y., Chen Y., Zhou R., Chen C., Tan J., Deng M., Dai Q., Yu X., Liu J., Zhang C., Wu W., Xu J., Dong S, & Luo F. (2021). Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioactive Materials, 6(7), 2039-2057.

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Western Blot Analysis of Cell Signaling

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Western blot analysis was performed as described previously. The antibodies used were as follows: rabbit anti-human DCP2 (1:1000; ab28658; abcam, Ltd), rabbit anti-human STAT3 (1:500; bsm-33218M; Bioss, Ltd), rabbit antihuman p-STAT3 (1:800; AP0248), mouse anti-human BIRC3 (1:500; MB0129), rabbit anti-human Bax (1:500; BS6420), Bcl2 (1:500; BS70205), rabbit anti-human Caspase 3 (1:1000; BS61583), rabbit anti-human Caspase 8(1:1000; AP0258), rabbit anti-human Caspase 9 (1:1000; AP0359), and rabbit anti-human GAPDH (1:3000; MB001) all from Bioworld Technology, Ltd).

Zhang Q., Yan Y.F., Lv Q., Li Y.J., Wang R.R., Sun G.B., Pan L., Hu J.X., Xie N., Zhang C., Tian B.C., Jiao F., Xu S., Wang P.Y, & Xie S.Y. (2021). miR-4293 upregulates lncRNA WFDC21P by suppressing mRNA-decapping enzyme 2 to promote lung carcinoma proliferation. Cell Death & Disease, 12(8), 735.

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