Ab15518

Immunoblotting was carried out in mice hippocampal tissues and primary rat culture in addition to the neuroblastoma cell line. Tissue homogenization and immunoblotting were performed as previously described [34 (link)]. The following primary antibodies were used: goat anti-S100A8 (sc-48352, Santa Cruz), rabbit anti-RAGE (ab3611, Abcam), rabbit anti-RXRγ (ab15518, Abcam), rabbit anti-NFk-B (PA1-186, Thermo Fischer), rabbit anti-actin (A2103, Sigma), and mouse anti-Lamin AC (SAB4200236, Sigma). Primary antibody incubation was followed by the incubation with the respective secondary immunoglobulin G (IgG) at 1:3000–5000 dilutions (Amersham Biosciences). Immunoreactivity was detected using the ECL detection system (Amersham Biosciences). The relative density of the immunoreactive bands was calculated from the optical density multiplied by the area of the selected band using the ImageJ 1.383 software (NIH, MA).

Primary antibodies used were chicken anti-GFP (ab13970, Abcam, 1:2000), rabbit anti-GFP (A-6455, Invitrogen, 1:500) mouse anti-ß-galactosidase (40-1a-s, DSHB, 1:20), chicken anti-ß-galactosidase (ab9361, Abcam, 1:1000) mouse anti-Visinin (7G4-s, DSHB, 1:250), mouse anti-Pax6 (Pax6-s, DSHB, 1:20), rabbit anti-PKC-alpha (P4334, Sigma, 1:500), mouse anti-AU1 (AU1, Biolegend, 1:1000) rabbit anti-MafA/L-Maf (gift from Celio Pouponnot, 1:500) [26 (link)], rabbit anti-Rxrg (ab15518, Abcam, 1:500), mouse anti-Rxrg (Santa Cruz Biotechnology SC-365252, 1:50), rabbit anti-cone arrestin (Millipore, AB15282, 1:2000), mouse anti-Nr2e3/PNR (R&D Systems, PP-H7223–00, 1:250). All secondary antibodies were obtained from Jackson Immunoresearch and were designated as appropriate for multiple labeling. Retinas were processed for staining as previously described [22 (link)].