siRNA target of Mettl3, UBC9 and scramble control were purchased from RIBOBIO. Transfection of siRNA duplexes was transfected using LipofectamineTM 2000 Transfection Reagent. The targets of the UBC9 siRNAs were 5'-TTGGCAGTAAATCGTGTAGGCC-3'(si#1) and 5'-ATTTAGAAGTTCCTGTATTCCT-3'(si#2), siMettl3 sequencing were 5'-GACTGCTCTTTCCTTAATA-3'(si#1) and 5'-GGACTCGACTACAGTAGCT-3'(si#2).
Scramble control
Manufactured by RiboBio
Sourced in China
Scramble control is a laboratory reagent used in gene expression analysis techniques such as RNA interference (RNAi) and quantitative real-time PCR (qRT-PCR). It serves as a negative control, providing a baseline for comparison to assess the specificity of experimental treatments or knockdown effects.
Automatically generated - may contain errors
Lab products found in correlation
Cxcl16 sirna, by Santa Cruz Biotechnology (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Plenti, by OriGene (1 mentions)
Agomir control, by GenePharma (1 mentions)
Mirzip control, by System Biosciences (1 mentions)
Streptavidin coupled agarose beads, by Thermo Fisher Scientific (1 mentions)
Scramble control, by GenePharma (1 mentions)
5 protocols using scramble control
1
Silencing METTL3 and UBC9 in Cells
2
CXCL16 knockdown in A549 cells
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CXCL16 siRNA (Santa Cruz, USA), Scramble control and positive control (Ribobio, China) were transfected into A549 cells by using Lipofectamine RNAiMAX (Invitrogen, USA) following the manufacturer's instructions, and the transfection efficiency was detected by western blot 48 h later.
3
Investigating miR-210 Regulation In Cells
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The miR-210 antagomir and scramble control were purchased from RiboBio Co., Guangzhou, China. The miR-210 agomir and agomir control were synthesized by GenePharma, Shanghai, China. The miR-Zip Control and miR-Zip-210 plasmids were purchased from System Bioscience, Palo Alto, CA, USA. The pLenti and pLenti-STMN1- Myc-DDKvectors were obtained from OriGene, Rockville, MD, USA. The pCMVΔR8.9, pDVsVg, and pLKO.1-shLuc vectors and the shRNA against STMN1 were obtained from the National RNAi Core Facility of Taiwan for gene silencing. All clones were verified by direct sequencing.
4
RNA Pull-Down Assay for miR-34a-5p
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RNA pull‐down assay was performed according to previous description.18 Scramble control, TUG1 sense, and antisense probes were biotin‐labeled from RiboBio Co. Ltd (Guangzhou, China). FLS cell lysates were prepared by RIPA buffer followed by incubation with probes for 2 h. Streptavidin‐coupled agarose beads (Thermo Fisher Scientific, Shanghai, China) were added into the reactions for 2 h to pull down the RNA‐RNA complex. The enrichment of miR‐34a‐5p in the RNA‐RNA complex was determined by qRT‐PCR. Experiments were performed in triplicate and repeated three times.
5
Characterization of ccRCC Cell Lines
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Eight human ccRCC cell lines (786‐0, 769‐p, A704, A498, ACNH, Caki‐1, Caki‐2, and RCC4) and an immortalized kidney proximal tubule epithelial cell line HK‐2 were obtained from the ATCC. Cell identities were authenticated by Sun Yat‐sen University with short tandem repeats profiling. All cells were maintained in RPMI modified medium containing 10% FBS and 1% penicillin/streptomycin (Hyclone).
All constructs including shRNA targeting LINC00973 and Siglec‐15 along with scramble control were obtained from GenePharma. The pcDNA 3.1 vector for Siglec‐15 overexpression and pLncEXP for LINC00973 expression were ordered from Synbio‐Tech. The oligonucleotides used in this study, including miR‐7109 mimic, miR‐7109‐specific inhibitor, and scramble control, were purchased from RiboBio. Cell transfection was performed with Lipofectamine 2000.
All constructs including shRNA targeting LINC00973 and Siglec‐15 along with scramble control were obtained from GenePharma. The pcDNA 3.1 vector for Siglec‐15 overexpression and pLncEXP for LINC00973 expression were ordered from Synbio‐Tech. The oligonucleotides used in this study, including miR‐7109 mimic, miR‐7109‐specific inhibitor, and scramble control, were purchased from RiboBio. Cell transfection was performed with Lipofectamine 2000.
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