Csb e12770m

Manufactured by Cusabio

Sourced in United States

The CSB-E12770m is a laboratory equipment product. It is designed to perform a specific function in a laboratory setting. This description is provided without interpretation or extrapolation on the intended use of the product.

Automatically generated - may contain errors

Lab products found in correlation

Csb e05101m, by Cusabio (2 mentions) Csb e06871m, by Cusabio (2 mentions) Csb e05104m, by Cusabio (1 mentions) Csb e13156m, by Cusabio (1 mentions) Csb e05109m, by Cusabio (1 mentions) Tunel kit, by Beyotime (1 mentions) C1086, by Beyotime (1 mentions) Ab242295, by Abcam (1 mentions) Ab118970, by Abcam (1 mentions) A015 2 1, by Nanjing Jiancheng (1 mentions) Versamax, by Molecular Devices (1 mentions) A001 3 2, by Nanjing Jiancheng (1 mentions) A005 1 2, by Nanjing Jiancheng (1 mentions)

4 protocols using csb e12770m

Serum Hormone Profiling by ELISA

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All blood samples were centrifuged at 1,000 g for 10 min and the serum was collected. Concentrations of follicle stimulating hormone (FSH), progesterone (P), luteinizing hormone (LH), and testosterone (T) in serum samples were determined by ELISA kit (CSB-E06871m, CSB-E05104m, CSB-E12770m, CSB-E05101m, CusaBio, Wuhan, China) according to the manufacturer's instructions, and all samples were repeated three times.

Yu Y., Cao Y., Huang W., Liu Y., Lu Y, & Zhao J. (2021). β-Sitosterol Ameliorates Endometrium Receptivity in PCOS-Like Mice: The Mediation of Gut Microbiota. Frontiers in Nutrition, 8, 667130.

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Mouse Hormonal Profile Analysis

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Blood samples were collected by cardiac puncture under anesthesia. Blood samples were centrifuged at 3000 rpm, and serum was separated and stored at −80°C for subsequent blood analysis. ELISA kits were used to measure mouse plasma follicle stimulating hormone (FSH) (CSB-E12770 m, CUSABIO), luteinizing hormone (LH) (CSB-E06871 m, CUSABIO), testosterone (T) (CSB-E05101 m, CUSABIO), anti-Müllerian hormone (AMH) (CSB-E13156 m, CUSABIO), and Irisin (DRE-M9930c, Kmaels) levels.

Zheng Y., He J., Yang D., Yuan M., Liu S., Dai F., Jia Y, & Cheng Y. (2022). Irisin reduces the abnormal reproductive and metabolic phenotypes of PCOS by regulating the activity of brown adipose tissue in mice. Biology of Reproduction, 107(4), 1046-1058.

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Enzymatic Synthesis of High-DHA Fish Oil

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Fish oil (DHA content > 80%) was obtained from Shanxi Taike Biotech Co., Ltd. (20200712-002, Xi’an, China). Rhizomucor miehei lipase (L8621) was obtained from Solarbio (Beijing, China). Ethyl dimethylaminopropyl carbodiimide (EDC), benzylamine, dichloromethane, HOBt ·H₂O, and triethylamine were obtained from Aladdin Co., Ltd. (Shanghai, China). The 3,3-diaminobenzidine kit (20×) (CW0125) was obtained from Cwbio Co., Ltd. (Beijing, China). Proteinase K (BL104A) and anti-fluorescence quenching mounting fluid (BL701A) were obtained from Biosharp Co., Ltd. (Hefei, China). Anti-FSHR (#40941) and anti-Müllerian-inhibiting factor (#42063) polyclonal antibodies were obtained from SAB Co., Ltd. (Baltimore, MD, USA).
Enzyme-linked immunosorbent assay (ELISA) kits for luteinizing hormone (LH; CSB-E12770m), follicle-stimulating hormone (FSH; CSB-E06871m), estradiol (E2; CSB-E05109m), and anti-Müllerian hormone (AMH; CSB-E13156m) were obtained from Cusabio Biotech Co., Ltd. (Wuhan, China). Evo M-MLV RT Mix Kit with gDNA Clean for qPCR (AG11728) and SYBR Green Pro Taq HS premixed qPCR kits (AG11701) were obtained from AgBio Co., Ltd. (Changsha, China). A TUNEL kit (in situ cell death detection; C1086) and DAPI (4′,6-diamidino-2-phenylindole; C1005) were obtained from Beyotime Biotech Co., Ltd. (Shanghai, China).

Guo L., Gao Q., Zhu J., Jin X., Yin H, & Liu T. (2022). A Docosahexaenoic Acid Derivative (N-Benzyl Docosahexaenamide) as a Potential Therapeutic Candidate for Treatment of Ovarian Injury in the Mouse Model. Molecules, 27(9), 2754.

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Hormones and Oxidative Stress in Mice

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Freshly collected blood samples from the mice were stood at room temperature for 2 h and centrifuged (1000g, 4°C) for 10 min. Serum hormones follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) were detected using the ELISA kit (CSB-E06871m, CSB-E12770m, CSB-E07280m, Cusabio, Wuhan, China) in strict accordance with the instructions. The absorbance was measured at 450 nm. The ovarian and oocytes sample lysis fluid were diluted to the optimal concentration to meet the standard curve for detection. Biomarkers related to oxidative stress including total antioxidant capacity (T-AOC), 8-hydroxy-2 deoxyguanosine (8-OH-dG), advanced oxidation protein products (AOPP), molondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) were detected by ELISA assay kit (A015-1-2, A001-3-2, A005-1-2, Jiancheng Bioengineering Institute, Nanjing, China; ab201734, ab242295, ab118970, Abcam, USA) following the manufacturer's instructions. The absorbance values were measured using a microplate reader (VersaMax, Molecular Devices, USA).

Fan L., Guan F., Ma Y., Zhang Y., Li L., Sun Y., Cao C., Du H, & He M. (2022). N-Acetylcysteine improves oocyte quality through modulating the Nrf2 signaling pathway to ameliorate oxidative stress caused by repeated controlled ovarian hyperstimulation. Reproduction, fertility, and development, 34(10).

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