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Ab176663

Manufactured by Abcam
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Ab176663 is a recombinant monoclonal antibody targeting the protein of interest. It is designed for use in various laboratory applications, including immunoassays and immunohistochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Ab197742, by Abcam (8 mentions) Ab222503, by Abcam (8 mentions) Ab208348, by Abcam (8 mentions)

8 protocols using ab176663

1

Cytokine Profiling in Mouse Cortex

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Mice were anesthetized and were transcranial perfused with 50 mL of cold NS. The temporal cortex was collected for assessment. The concentrations of proinflammatory cytokines were detected. There were five supernatants in each group for ELISA analysis. An ELISA was used as directed by the manufacturer to determine the levels of IL‑1β (cat. no. ab197742; Abcam), IL‑6 (cat. no. ab222503; Abcam), TNF‑α (cat. no. ab208348; Abcam), and NF‑κB (cat. no. ab176663; Abcam). The specific experimental procedure according to the guidelines stipulated by the manufacturer and previous study [45 (link)].
Wu Y., Zhang J., Feng X, & Jiao W. (2023). Omega-3 polyunsaturated fatty acids alleviate early brain injury after traumatic brain injury by inhibiting neuroinflammation and necroptosis. Translational Neuroscience, 14(1), 20220277.
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2

Quantifying Inflammatory Markers via ELISA

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The levels of interleukin-1β (IL-1β) (cat. no. ab197742; Abcam), IL-6 (cat. no. ab222503; Abcam), tumor necrosis factor-α (TNF-α) (cat. no. ab208348; Abcam), and NF-κB (cat. no. ab176663; Abcam) were measured by enzyme-linked immunoassay (ELISA) according to the manufacturer’s instructions37 (link).
Wu X., Jiao W., Chen J., Tao Y., Zhang J, & Wang Y. (2022). Ulinastatin alleviates early brain injury after intracerebral hemorrhage by inhibiting oxidative stress and neuroinflammation via ROS/MAPK/Nrf2 signaling pathway. Acta Cirúrgica Brasileira, 37(6), e370606.
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3

Inflammatory Cytokine Measurement by ELISA

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The levels of interleukin-1β (IL-1β) (cat. no. ab197742; Abcam), IL-6 (cat. no. ab222503; Abcam), tumor necrosis factor-α (TNF-α) (cat. no. ab208348; Abcam), and NF-κB (cat. no. ab176663; Abcam) were measured by enzyme-linked immunoassay (ELISA) according to the manufacturer’s instructions31 (link).
Lu W., Zhu Z., Shi D., Li X., Luo J, & Liao X. (2022). Cerebrolysin alleviates early brain injury after traumatic brain injury by inhibiting neuroinflammation and apoptosis via TLR signaling pathway. Acta Cirúrgica Brasileira, 37(6), e370605.
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4

Cytokine and NF-κB Quantification

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IL-1β (cat. no. ab197742; Abcam), IL-6 (cat. no. ab222503; Abcam), TNF-α (cat. no. ab208348; Abcam) and NF-κB (cat. no. ab176663; Abcam) levels were measured using ELISAs according to the manufacturer's instructions.
Feng X., Ma W., Zhu J., Jiao W, & Wang Y. (2021). Dexmedetomidine alleviates early brain injury following traumatic brain injury by inhibiting autophagy and neuroinflammation through the ROS/Nrf2 signaling pathway. Molecular Medicine Reports, 24(3), 661.
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5

Cytokine and NF-κB Measurement in Samples

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The levels of interleukin (IL)-1β (cat. no. ab197742; Abcam), IL-6 (cat. no. ab222503; Abcam), tumour necrosis factor (TNF)-α (cat. no. ab208348; Abcam) and NF-κB (cat. no. ab176663; Abcam) were measured using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions.
Feng X., Ma W., Chen J., Jiao W, & Wang Y. (2022). Ulinastatin alleviates early brain injury after traumatic brain injury by inhibiting oxidative stress and apoptosis. Acta Cirúrgica Brasileira, 37(1), e370108.
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6

Inflammatory Cytokines in Intracerebral Hemorrhage

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The ipsilateral cortex tissue in animals and ICH patients’ serum were collected
and detected. The levels of IL-1β (cat. No. ab197742; Abcam), IL-6 (cat. No.
ab222503; Abcam), TNF-α (cat. No. ab208348; Abcam), and NF-κB (cat. No.
ab176663; Abcam) were measured by the enzyme-linked immunosorbent assay (ELISA),
according to the manufacturer’s instructions.
Wang L., Jiao W., Wu J., Zhang J., Tang M, & Chen Y. (2022). Ulinastatin alleviates early brain injury after intracerebral hemorrhage by inhibiting necroptosis and neuroinflammation via MAPK/NF-κB signaling pathway. Acta Cirúrgica Brasileira, 37(3), e370301.
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7

Inflammatory Cytokine Profiling by ELISA

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The levels of IL-1β (cat. no. ab197742; Abcam), IL-6 (cat. no. ab222503; Abcam), TNF-α (cat. no. ab208348; Abcam), and NF-κB (cat. no. ab176663; Abcam) were measured by ELISA, according to the manufacturer's instructions.
Yang L., Wang Y., Zhang C, & Cheng H. (2021). Perampanel, an AMPAR antagonist, alleviates experimental intracerebral hemorrhage-induced brain injury via necroptosis and neuroinflammation. Molecular Medicine Reports, 24(2), 544.
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8

Cytokine Levels in Cerebral Cortex

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The ELISA kit was utilized following the guidelines stipulated by the manufacturer to determine the levels of cerebral cortex NF-κB (cat. no. ab176663; Abcam), TNF-α (cat. no. ab208348; Abcam), IL-6 (cat. no. ab222503; Abcam), and IL-1β (cat. no. ab197742; Abcam).
Zhang C., Shi Z., Xu Q., He J., Chen L., Lu Z., Huan Q., Wang Y, & Cui G. (2023). Astragaloside IV alleviates stroke-triggered early brain injury by modulating neuroinflammation and ferroptosis via the Nrf2/HO-1 signaling pathway. Acta Cirúrgica Brasileira, 38, e380723.
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