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2 protocols using nanopure system

1

Polyelectrolyte Multilayer Coatings

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Poly(isobutylene-alt-maleic anhydride) (PIAMA, Mw: 6 kDa), poly(allylamine hydrochloride) (PAH, Mw: 58 kDa), l-histidine methyl ester dihydrochloride, 3-aminopropyltrimethoxysilane (APTMS), N,N-diisopropylethylamine (DIPEA), copper(ii) nitrate trihydrate (Cu(NO3)2 × 3H2O), sodium chloride, sea salt and sodium hydroxide (all from Sigma Aldrich), N,N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), toluene, methanol, acetone and isopropanol (all from Tedia) were used directly as received without further purification. Dialysis membrane tubing (MWCO: 3.5 kD) was received from Fisher Scientific. Silicon wafers (Latech Scientific Supply Pte. Ltd) were 0.6 mm thick, with one side polished and with a natural silicon dioxide layer. QSX 303 Silicon dioxide 50 nm quartz crystal microbalance (QCM) chips were obtained from Analytical Technologies Pte Ltd. Deionized (DI) water with 18 MΩ cm–1 resistivity was obtained from a Millipore Nanopure system.
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2

Protein Binding Assay Reagents

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The AGP (from pooled human serum, 99% pure), periodic acid reagent (H5IO6), glycogen (bovine liver), HSA (Cohn fraction V, 99% globulin free, 99% fatty acid free), amitriptyline, carbamazepine, chloramphenicol, chlorpromazine, disopyramide, imipramine, lidocaine, nortriptyline, S-propranolol, and quinidine were from Sigma-Aldrich (St. Louis, MO, USA). The Nucleosil silica (7 μm particle diameter, 100 or 300 Å pore size) was obtained from Macherey Nagel (Düren, Germany). Reagents for the micro bicinchoninic (BCA) protein assay were from Pierce (Rockford, IL, USA). All other chemicals were of the purist grades available. All solutions were prepared using water from a Barnstead NANOpure system (Dubuque, IA, USA) or a Milli-Q Advantage A10 purification system (Millipore, Billerica, MA, USA) and were filtered through 0.20 μm GNWP nylon membranes from Millipore.
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