At different time points, the repaired knees (n = 5 in each group) were harvested and fixed in 10% neutral buffered formalin for 48 h. Subsequently, whole specimens were demineralized in a decalcifying solution (ZSGB-BIO, Beijing, China) for two weeks. The decalcified specimens were then trimmed, dehydrated in a graded ethanol series, and embedded in paraffin. Serial sections of 5-μm thickness were cut and stained with hematoxylin and eosin (H&E) and toluidine blue (TB). Immunohistochemistry analysis was performed using antibodies against type II collagen (Invitrogen, MA5-13026, Carlsbad, CA, United States), type I collagen (Sigma-Aldrich, C2456, St. Louis, MO, United States), and type X collagen (Abcam, ab49945). A modified scoring system (Supplementary Table S3 ) was used to assess the histological repair outcomes of articular cartilage defects (Wakitani et al., 1994 (link)). Histological evaluation was performed by the same 2 observers in a blinded manner.
Ma5 13026
Manufactured by Merck Group
Sourced in United States
The MA5-13026 is a laboratory equipment product manufactured by Merck Group. It is designed for specific technical functions within a laboratory setting. As a Marketing Specialist, I can provide a factual and unbiased description of the core function of this product, without making any interpretations or extrapolations about its intended use.
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Lab products found in correlation
2 protocols using ma5 13026
1
Histological Analysis of Articular Cartilage Repair
2
Immunohistochemical Evaluation of Cartilage Markers
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For immunohistochemical evaluation, primary antibodies, i.e., type 1 collagen (Arigo, ARG-21965), type 2 collagen (Invitrogen, MA5-13026), aggrecan (Sigma, C8035) and SOX9 (Millipore Sigma, HPA001758) were used. Donkey anti-goat IgG H&L (HRP) (Abcam, ab6885), Goat anti-rabbit IgG H&L (HRP) (Abcam, ab6721) and anti-mouse IgG H&L (HRP) (Abcam, ab6789) were used as secondary antibodies. Antigen retrieval was completed by incubation with 0.4% pepsin (Aladdin, P110928, Shanghai, China) at 37 °C for 1 h. Endogenous peroxidase was blocked by incubation with 3% H2O2 for 15 min, and nonspecific protein binding was blocked by incubation with goat serum (Boster, AR0009, China) for 1 h. The sections were incubated with primary antibodies for 2 h at room temperature and then incubated with secondary antibodies for 1 h at room temperature. Finally, the color was developed by using diaminobenzidine (DAB) substrate kit.
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