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Gfp lc3 plasmid

Manufactured by Cell Biolabs
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Sourced in China

The GFP-LC3 plasmid is a recombinant DNA construct that expresses a fusion protein between the green fluorescent protein (GFP) and the microtubule-associated protein 1A/1B-light chain 3 (LC3). LC3 is a widely used marker for autophagosomes, which are double-membrane vesicles involved in the autophagy process. The GFP-LC3 plasmid can be used to monitor the formation and trafficking of autophagosomes in living cells.

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Lab products found in correlation

Opti mem 1 reduced, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

2 protocols using gfp lc3 plasmid

1

Overexpressing TFEB Modulates Cd Toxicity

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According to the manufacturer’s instructions, cells were transfected with Opti-MEM® I reduced serum media and Lipofectamine 2000 (Invitrogen, 11668–019). For Tfeb overexpression, two plasmids, pEGFP-N1-TfebS141A, pEGFP-N1-TfebS141D, and control plasmids designed by Invitrogen Corporation (Shanghai, China) as well as GFP-LC3 plasmid (Cell Biolabs, CBA-401) were transfected into Neuro-2a cells. At 24 h after transfection, the cells were exposed to 50 μM Cd for 24 h.
Pi H., Li M., Tian L., Yang Z., Yu Z, & Zhou Z. (2017). Enhancing lysosomal biogenesis and autophagic flux by activating the transcription factor EB protects against cadmium-induced neurotoxicity. Scientific Reports, 7, 43466.
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2

Plasmids, Autophagy, and TFEB Silencing

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Plasmids (HPA1, E225/343A mutant, and HPA knockdown) were obtained from Gene (Shanghai, China). GFP-LC3 plasmid and Premo™ Autophagy Tandem Sensor RFP-GFP-LC3B Kit were purchased from Cell Biolabs and Life Technologies, respectively. siRNA for TFEB was purchased from Ribobio (Guangzhou, China).
Yang M., Tang B., Wang S., Tang L., Wen D., Vlodavsky I, & Yang S.M. (2022). Non-enzymatic heparanase enhances gastric tumor proliferation via TFEB-dependent autophagy. Oncogenesis, 11(1), 49.
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