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Fitc conjugated anti mouse iga

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FITC-conjugated anti-mouse IgA is a lab equipment product that is used to detect and quantify mouse IgA antibodies in various applications, such as ELISA and flow cytometry. It consists of fluorescein isothiocyanate (FITC) conjugated to antibodies that specifically bind to mouse IgA.

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Lab products found in correlation

Lsrii flow cytometer, by BD (2 mentions) Anti cd40, by Thermo Fisher Scientific (2 mentions) Interleukin 4 (il 4), by R&D Systems (2 mentions) Tgf β1, by R&D Systems (2 mentions) Anti flag, by Merck Group (1 mentions) Anti h2a, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti chk1, by Santa Cruz Biotechnology (1 mentions) Anti h2ax s139p, by Cell Signaling Technology (1 mentions) Anti rad52, by Santa Cruz Biotechnology (1 mentions) Anti ras, by Santa Cruz Biotechnology (1 mentions) Anti rpa32, by Fortis Life Sciences (1 mentions) Brca1, by Fortis Life Sciences (1 mentions) Anti fanca, by Fortis Life Sciences (1 mentions) Anti rad51, by Santa Cruz Biotechnology (1 mentions) Aperio cs2, by Leica (1 mentions) Lsm 710 meta, by Zeiss (1 mentions)

Spelling variants of this product

Anti-IgA-FITC (6 citations) IgA-FITC (4 citations) FITC-conjugated anti-mouse IgA antibody (3 citations) FITC-conjugated anti-IgA (2 citations) Anti-mouse IgA (2 citations) Mouse anti (2 citations)

5 protocols using fitc conjugated anti mouse iga

1

CSR Efficiency Assay in CH12F3 Cells

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CH12F3 cells were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum and 50 μM of beta-mercaptoethanol. For CSR assay, healthy CH12F3 cells were seeded at 5 × 104 cells/ml in the presence of 1 μg/ml anti-CD40 (eBioscience 16-0402-86), 5 ng/ml of IL-4 (R&D Systems 404-ML), and 0.5 ng/ml TGF-β1 (R&D Systems 240-B) and grown for 72 hr. Cells were stained with a FITC-conjugated anti-mouse IgA (BD Biosciences 559354) and analyzed on a LSR II flow cytometer (BD Biosciences). CSR efficiency is determined as the percentage of IgA-positive cells.
Han L., Masani S., Hsieh C.L, & Yu K. (2014). DNA Ligase I Is Not Essential for Mammalian Cell Viability. Cell reports, 7(2), 316-320.
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2

Immunoblotting analysis of DNA repair proteins

Cited 3 times
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Whole cell lysis and immunoblotting were performed as described63 (link),68 (link). Antibodies against FANCM and FAAP24, and antibody against BRCA2 were kindly provided by Dr. Weidong Wang38 (link) and Dr. Jun Huang66 (link), respectively. Antibodies against Mre11 and CtIP were used previously10 (link),63 (link),69 (link). Commercial antibodies used were: Anti-FANCA (Bethyl Laboratories A301-980A,1:1000), anti-FANCD2 (Bethyl Laboratories A302-174A, 1:1000), anti-MHF1 (Abcam, ab169385, 1:1000), anti-Rad51 (Santa Cruz Biotechnology, sc-398587, 1:500), anti-H2AX-S139p (Cell Signaling, #2577, 1:1000), anti-H2A (Cell signaling, #2578, 1:1000), anti-FLAG (Sigma, F1804, 1:2000), anti-Rad52 (Santa Cruz Biotechnology, sc-365341, 1:1000), anti-Ras (Santa Cruz Biotechnology, sc-520, 1:1000), anti-β-actin (Sigma, A5441, 1:2000), anti-Chk1 (Santa Cruz Biotechnology, sc-8408, 1:500), anti-Chk1-S345p (Cell Signaling,#2348, 1:500), anti-RPA32 (Bethyl Laboratories A300-244A, 1:1000), BRCA1 (Bethyl Laboratories, A300–000A, 1:500) and FITC-conjugated anti-mouse IgA (BD, 559354).
Wang H., Li S., Oaks J., Ren J., Li L, & Wu X. (2018). The concerted roles of FANCM and Rad52 in the protection of common fragile sites. Nature Communications, 9, 2791.
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3

Assessing Nasal Immunoglobulin A Response

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The areas around the nasal tissues were excised at 7 d after the immunization, perfused in 4% paraformaldehyde, dehydrated in 10%, 20%, and 30% sucrose solutions, and then frozen sectioned (20 µm) for FITC‐conjugated antimouse IgA (BD Biosciences) counter staining and H&E staining. Frozen sections from immunized mice were subjected to a blocking step with 1% BSA and were stained with FITC‐conjugated antimouse IgA antibody. The sections were then counterstained with DAPI and analyzed using confocal microscopy (LSM 710 Meta, Carl Zeiss, Germany). For H&E staining, the sectioned nasal tissues were stained with H&E for the nucleus and cytoplasm. The stained sections were captured using slide scanner (APERIO CS2, Leica Biosystems, Germany).
Jeong H., Lee C., Lee J., Lee J., Hwang H.S., Lee M, & Na K. (2021). Hemagglutinin Nanoparticulate Vaccine with Controlled Photochemical Immunomodulation for Pathogenic Influenza‐Specific Immunity. Advanced Science, 8(23), 2100118.
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4

Visualizing Intestinal IgA-Bacteria Interactions

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E. coli HA107 was cultured without shaking at 37°C for overnight. The bacteria were then washed in PBS and resuspended at 107 per ml. Portions (50 μl) of the bacterial suspension were dropped onto glass slides and the bacterial drop was encircled using a wax pen. Bacteria were allowed to sediment onto the glass slides and to dry. Transiently flamed bacteria on glass slides were stained with intestinal wash prepared from small intestine of HA107 conditioned germ-free C57BL/6 mice. The IgA antibodies bound to bacteria were visualized with FITC-conjugated anti-mouse IgA (BD Bioscience). DAPI (4’, 6-diamidino-2-phenylindole) was used to counterstain the bacterial DNA. Immunofluorescent pictures were taken using Flash Slide Scanner (PerkinElmer) with 40 magnifications.
Uchimura Y., Fuhrer T., Li H., Lawson M.A., Zimmermann M., Yilmaz B., Zindel J., Ronchi F., Sorribas M., Hapfelmeier S., Ganal-Vonarburg S.C., Gomez de Agüero M., McCoy K.D., Sauer U, & Macpherson A.J. (2018). Antibodies Set Boundaries Limiting Microbial Metabolite Penetration and the Resultant Mammalian Host Response. Immunity, 49(3), 545-559.e5.
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5

CSR Efficiency Assay in CH12F3 Cells

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CH12F3 cells were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum and 50 μM of beta-mercaptoethanol. For CSR assay, healthy CH12F3 cells were seeded at 5 × 104 cells/ml in the presence of 1 μg/ml anti-CD40 (eBioscience 16-0402-86), 5 ng/ml of IL-4 (R&D Systems 404-ML), and 0.5 ng/ml TGF-β1 (R&D Systems 240-B) and grown for 72 hr. Cells were stained with a FITC-conjugated anti-mouse IgA (BD Biosciences 559354) and analyzed on a LSR II flow cytometer (BD Biosciences). CSR efficiency is determined as the percentage of IgA-positive cells.
Han L., Masani S., Hsieh C.L, & Yu K. (2014). DNA Ligase I Is Not Essential for Mammalian Cell Viability. Cell reports, 7(2), 316-320.
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