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Human nucleofection kit

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The Human Nucleofection Kit is a laboratory tool designed for the transfection of DNA, RNA, or other macromolecules into human cells. The kit utilizes an electroporation-based technology to facilitate the efficient delivery of these molecules into the cell nucleus, enabling various research applications.

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Nucleofection (130 citations) Nucleofection Kit (19 citations) Human monocyte nucleofection kit (6 citations) Human T-cell nucleofection kit (5 citations) Amaxa Human T cell Nucleofection kit (4 citations) Amaxa Human Stem Cell Nucleofection Kit (3 citations) Amaxa Human B-cell Nucleofection Kit (3 citations) Human Primary Cell Nucleofection Kit P3 (2 citations) Human chondrocyte nucleofection kit (2 citations) Human stem cell nucleofection kit (2 citations)

4 protocols using human nucleofection kit

1

MicroRNA Functional Analysis in T Cells

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MicroRNAs were analyzed with microRNA TaqMan assays (Life Technologies) accordingly to the manufacturer's instruction. To examine the roles of microRNAs, we transfected T cells with specific microRNA mimics or irrelevant oligonucleotide controls on day 2 using human nucleofection kit (Lonza). The cells were analyzed on day 3.
Zhao E., Maj T., Kryczek I., Li W., Wu K., Zhao L., Wei S., Crespo J., Wan S., Vatan L., Szeliga W., Shao I., Wang Y., Liu Y., Varambally S., Chinnaiyan A.M., Welling T.H., Marquez V.E., Kotarski J., Wang H., Wang Z., Zhang Y., Liu R., Wang G, & Zou W. (2015). Cancer mediates effector T cell dysfunction by targeting microRNAs and EZH2 via glycolysis restriction. Nature immunology, 17(1), 95-103.
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2

MicroRNA Functional Analysis in T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
MicroRNAs were analyzed with microRNA TaqMan assays (Life Technologies) accordingly to the manufacturer's instruction. To examine the roles of microRNAs, we transfected T cells with specific microRNA mimics or irrelevant oligonucleotide controls on day 2 using human nucleofection kit (Lonza). The cells were analyzed on day 3.
Zhao E., Maj T., Kryczek I., Li W., Wu K., Zhao L., Wei S., Crespo J., Wan S., Vatan L., Szeliga W., Shao I., Wang Y., Liu Y., Varambally S., Chinnaiyan A.M., Welling T.H., Marquez V.E., Kotarski J., Wang H., Wang Z., Zhang Y., Liu R., Wang G, & Zou W. (2015). Cancer mediates effector T cell dysfunction by targeting microRNAs and EZH2 via glycolysis restriction. Nature immunology, 17(1), 95-103.
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3

EZH2 Overexpression in Activated T Cells

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T cells were transfected with EZH2-pCMV plasmid27 (link),41 (link) before activation with human nucleofection kit (Lonza).
Zhao E., Maj T., Kryczek I., Li W., Wu K., Zhao L., Wei S., Crespo J., Wan S., Vatan L., Szeliga W., Shao I., Wang Y., Liu Y., Varambally S., Chinnaiyan A.M., Welling T.H., Marquez V.E., Kotarski J., Wang H., Wang Z., Zhang Y., Liu R., Wang G, & Zou W. (2015). Cancer mediates effector T cell dysfunction by targeting microRNAs and EZH2 via glycolysis restriction. Nature immunology, 17(1), 95-103.
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4

EZH2 Overexpression in Activated T Cells

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T cells were transfected with EZH2-pCMV plasmid27 (link),41 (link) before activation with human nucleofection kit (Lonza).
Zhao E., Maj T., Kryczek I., Li W., Wu K., Zhao L., Wei S., Crespo J., Wan S., Vatan L., Szeliga W., Shao I., Wang Y., Liu Y., Varambally S., Chinnaiyan A.M., Welling T.H., Marquez V.E., Kotarski J., Wang H., Wang Z., Zhang Y., Liu R., Wang G, & Zou W. (2015). Cancer mediates effector T cell dysfunction by targeting microRNAs and EZH2 via glycolysis restriction. Nature immunology, 17(1), 95-103.
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