Nicolet ir200

HPLC was carried on LDC Analytical Waters, USA, equipped with Diode-array UV–Visible detector and auto sampler injector. The chromatographic analysis was carried out using EZ Chrome Elite data analysis program and Waters X-bridge Shield RP₁₈ column [(150 × 3.9 × 5 μm particle size) B.N: WAT046980, (S.N:0200343221)]. Camag Micro syringe (100 μL) Lino mat autosampler is from Camag^® company, Automatic TLC Sampler 4 (ATS 4), Muttenzl, Switzerland. Camag TLC scanner (35/N/30319) with win CATS software; an ultraviolet (UV) lamp with a short wavelength at 250 nm (Desaga, Wiesloch, Germany). TLC analysis was carried on Aluminum TLC plates precoated with silica gel 60 GF₂₅₄ (20 × 20 cm, Merck, Darmstadt, Germany) with its chromatographic tank (25 × 25 × 9 cm). Other equipment used: hot plate (Torrey pines Scientific, USA); Jenway, 3510 pH meter (Jenway, USA); Scilogex Rotatory evaporator; NMR, Gemini-400 BB (Agilent, USA); FT-IR, Nicolet IR 200 (Thermo electron corporation, USA), with MS-QP-1000 EX mass spectrometer at 70 eV (Shimadzu, Tokyo, Japan); molecular operating environments (MOE) {2015:10} software (Chemical Computing Group, Montreal, QC, Canada) were used.

7,16-Bis[2-(3-bromopropoxy)benzoyl]-5,14-dihydrodibenzo[b,i][1,4,8,11]tetraazacyclotetradecine, 7,16-Bis[2-hydroxybenzoyl]-5,14-dihydrodibenzo[b,i][1,4,8,11]tetraazacyclotetradecine and 9-(6-bromopentyl)adenine were prepared by the procedure described earlier [13 (link)–15 (link)]. All reagents were purchased from commercial sources (Sigma-Aldrich) and were used as received. Solvents were dried by using standard methods and were freshly distilled before use.
¹H and ¹³C NMR were run on Bruker AVANCE II 300 and Bruker AVANCE III 600 spectrometers. Chemical shifts (δ) are expressed in parts per million and J values in hertz. Signal multiplicities are denoted as s (singlet), d (doublet), t (triplet), q (quartet), and m (multiplet). The IR-ATR spectra were recorded with a Thermo Fisher Scientific Nicolet IR200. ESI mass spectra were taken on a Bruker Daltonics microTOF-II spectrometer.

The FTIR spectrum of G. lucidum BG was analyzed on an FTIR spectrometer (Thermo Scientific Nicolet IR200, Waltham, MA, USA) by a total of 128 scans accumulated in attenuated total reflection (ATR) mode with a resolution of 4 cm⁻¹. The spectrum was obtained in the range of 4000 to 400 cm⁻¹.

Melting points were measured on a Griffin melting point apparatus (Griffin, Valdosta, GA, USA) and are uncorrected. The infrared spectra were recorded as KBr discs on a Nicolet IR 200 (Thermo Fisher Scientific, Barrington, RI, USA). The ¹H-NMR and ¹³C-NMR spectra were run using TMS as an internal standard (Aldrich chemical Co., Milwaukee, WI, USA) on a Varian Mercury VXr-300 NMR (Varian, Palo Alto, CA, USA). Mass spectra were measured on a JEOL-SX-102 instrument using electron impact ionization. Elemental analysis were performed on a Perkin-Elmer 240C analyzer (Perkin-Elmer, Norwalk, CT, USA) at the Analytical Center, College of Science, Cairo University, Egypt. All values were within ±0.4% of the theoretical values. The reactions were monitored by thin-layer chromatography (TLC) using TLC sheets precoated with UV fluorescent silica gel Merck 60 F254 plates and were visualized using a UV lamp and different solvents as mobile phases. All chemicals were purchased from (Sigma-Aldrich, Milwaukee, WI, USA).

The crystalline nature of nickel oxide nanoparticles was investigated by X–ray diffraction technique. The XRD patterns of the prepared samples were recorded with the help of Bruker D2 PHASER X–ray diffractometer using monochromatised CuK_α1 radiation at a wavelength of 1.54060 Å. IR spectra were recorded with Thermo Nicolet IR 200 (USA). Thermal behavior was studied using differential scanning calorimeter Universal V4.5A, TA instruments USA, varying the temperature from room temperature to 1000 °C at a rate of 10 °C/min in nitrogen atmosphere. Structural studies of the samples were done by FESEM FEI Nova 450 NanoSEM.

Attenuated total reflection Fourier transform spectroscopy (ATR-FTIR) was employed to characterize the methacrylate-functionalized calcium phosphate (MCP) and the precipitated layer formed on the composite after immersion in PBS. The spectra of the specimens were obtained using a Nicolet IR200 spectrophotometer (Thermo-Fisher, Waltham, MA, USA) with an ATR accessory and were recorded in absorption mode using 4 cm⁻¹ resolution with 32 scans and in the range of 500–4000 cm⁻¹. The FTIR of the surface precipitated layer was measured by scraping off that layer from the specimens after being stored in phosphate buffered saline (PBS) for 21 days at 37 °C.

Fourier transform infrared (FTIR) spectrometer (Thermo Scientific Nicolet IR200, Waltham, MA, USA) was used to investigate the chemical functional groups on the surface of paper samples. FTIR spectra were collected over the range of 400–4000 cm⁻¹, 128 scans and a resolution of 4 cm⁻¹ in attenuated total reflection (ATR) mode.