Paraffin block was cut into 4-μm sections and treated by routine skill. After microwaved in citrate buffer for 5 min, the slides were incubated with Foxp3 (ab10563, Abcam, USA), VEGF-C (sc-374,628, Santa Cruz, CA), and D2-40 (MAB-0567, Maxim-Bio, Fuzhou, China) at room temperature respectively. Then, the sections were incubated with a secondary antibody (MaximBio Company, Fuzhou, China). Labeling was monitored by diaminobenzidine (Maxim-Bio Company). At last, hematoxylin was used to stain the sections.
Vegf c sc 374 628
Manufactured by Santa Cruz Biotechnology
Sourced in United States
VEGF-C (sc-374,628) is a recombinant protein product manufactured by Santa Cruz Biotechnology. It is a member of the vascular endothelial growth factor (VEGF) family, which are key regulators of angiogenesis and lymphangiogenesis. The core function of VEGF-C is to stimulate the proliferation and migration of endothelial cells.
Automatically generated - may contain errors
Lab products found in correlation
Ab10563, by Abcam (1 mentions)
Chemiluminescence reagent, by Santa Cruz Biotechnology (1 mentions)
Vegf c, by Santa Cruz Biotechnology (1 mentions)
Immobilon p transfer membrane, by Merck Group (1 mentions)
β actin sc 58673, by Santa Cruz Biotechnology (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using vegf c sc 374 628
1
Immunohistochemical Analysis of Foxp3, VEGF-C, and D2-40
2
Western Blot Analysis of VEGF-C
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Clinical tissues or cultured cells were lysed with RIPA buffer (Beyotime Biotechnology, China). Equal amounts of proteins were separated by 10% polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto Immobilon-P Transfer membrane (Merck Millipore, China). Immunoblots were prepared using standard protocols including BSA blocking, primary antibody incubation (VEGF-C, sc-374628; β-actin, sc-58673; both from Santa Cruz Biotechnology, USA), secondary horseradish peroxidase-conjugated antibody incubation (anti-goat IgG for VEGF-C, anti-mouse IgG for TBL1XR1 and β-actin) and eventually developed with chemiluminescence reagents (Santa Cruz Biotechnology, USA).
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