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Anti cd62p apc

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Anti-CD62P-APC is a fluorescently labeled monoclonal antibody that binds to the CD62P antigen, also known as P-selectin, on the surface of activated platelets and endothelial cells. It is commonly used in flow cytometry applications to detect and quantify the presence of activated platelets.

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Lab products found in correlation

Fibrinogen, by Enzyme Research (3 mentions) Anti cd41 v450, by BD (1 mentions) Canto 2 special order research product, by BD (1 mentions) Anti pac 1 fitc, by BD (1 mentions) Supersignal west dura substrate, by Thermo Fisher Scientific (1 mentions) Anti cd41 pe, by BD (1 mentions) Cytofixbd, by BD (1 mentions) Anti cd31 efluor450, by Thermo Fisher Scientific (1 mentions) Seakem gold agarose, by Lonza (1 mentions) Thrombin, by Merck Group (1 mentions) U73343, by Bio-Techne (1 mentions) U73122, by Bio-Techne (1 mentions) P gsk3β ser9, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Mrs2395, by Merck Group (1 mentions) Rottlerin, by Bio-Techne (1 mentions) Ro 31 8220, by Bio-Techne (1 mentions) Bapta, by Bio-Techne (1 mentions) Trap 6, by Bio-Techne (1 mentions) Ar c66096, by Bio-Techne (1 mentions)

3 protocols using anti cd62p apc

1

Platelet Activation Monitoring by Flow Cytometry

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Platelet-rich plasma was prepared immediately following blood sample collection and used for monitoring platelet activation markers. Five µL of platelet-rich plasma were incubated 30 min at room temperature in the dark with 3 µL of anti-CD41-V450 (BD Bioscience Canada, Mississauga, Ontario, Canada), a marker of platelets, 15 µL of anti-CD62P-APC and 15 µL of anti-PAC1-FITC (BD Bioscience Canada, Mississauga, Ontario, Canada) and anti-ATX (BD Bioscience Canada, Mississauga, Ontario, Canada) in 100 µL of phosphate buffered saline(PBS). The samples were mixed with 400 µL of PBS to stop labelling and analysed using a high sensitivity flow cytometer BD Canto II Special Order Research Product with the gating strategy described in online supplemental figure 1A. The flow cytometer settings were as follows: FSC at 300 V, SSC at 335 V, Pacific blue at 500 V, FITC at 500 V, PE at 500 V and APC at 500 V.
Hasse S., Julien A.S., Duchez A.C., Zhao C., Boilard E., Fortin P.R, & Bourgoin S.G. (2022). Red blood cell-derived phosphatidylserine positive extracellular vesicles are associated with past thrombotic events in patients with systemic erythematous lupus. Lupus Science & Medicine, 9(1), e000605.
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2

Platelet Activation and Adhesion Assays

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Cross-linked collagen-related protein/CRP-XL was purchased from University of Cambridge (Cambridge, England). Thromboxane A2 analog/U46619 and PAR-1 agonist/TRAP6 were from Tocris (Bristol, England). Epinephrine and fibrillar collagen were from Chrono-Log (Havertown, PA). Human VWF was from Haematologic Technologies, Inc (Essex Junction, VT). Fibrinogen was from Enzyme Research Laboratories (South Bend, IN). PPACK, rabbit anti-human anti-VWF primary antibody and goat anti-human anti-Vinculin primary antibodies were from Santa Cruz (Dallas, TX). Anti-CD41-PE, anti-CD62P-APC and CytofixBD were from BD Pharmingen (Franklin Lakes, NJ). Anti-CD31-eFluor450 was from eBioscience (San Diego, CA). Mouse anti-human anti-GPIb/AK2 primary antibody was from GeneTex (Irvine, CA) and anti-mouse IgG -AF640 secondary was from Invitrogen (Carlsbad, CA). Seakem gold agarose was from Lonza (Basel, Switzerland). Recombinant rh-ADAMTS13 was form R&D Systems (Minneapolis, MN). SuperSignal West Dura Substrate was from Thermo Scientific (Waltham, MA). Other reagents were purchased from Sigma (St. Louis, MO).
Zilberman-Rudenko J., Zhao F.Z., Reitsma S.E., Mitrugno A., Pang J., Shatzel J.J., Rick B., Tyrrell C., Hasan W., McCarty O.J, & Schreiber M.A. (2018). Effect of pneumatic tubing system transport on platelet apheresis units. Cardiovascular engineering and technology, 9(3), 515-527.
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3

Platelet activation signaling pathways

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MRS2395 [2,2-Dimethyl-propionic acid 3-(2-chloro-6-methylaminopurin-9-yl)-2-(2,2-dimethyl-propionyloxymethyl)-propyl
ester], adenosine diphosphate (ADP), wortmannin, thrombin and all other chemicals and reagents were purchased from Sigma-Aldrich
(St Louis, MO, USA) or previously mentioned sources unless specified otherwise.[12 (link), 13 (link)] TRAP-6 (SFLLRN), U73122, U73343, Ro 31–8220, Rottlerin, Go6976, MRS2179, BAPTA,
PSB 0739 and AR-C 66096 were obtained from Tocris (Bristol, UK). Ticagrelor was purchased from Oxchem Corporation (Wood Dale, IL,
USA). AYPGKF-NH2 was obtained from Abgent (San Diego, CA, USA). Oregon Green® 488 BAPTA-1 AM (OG488 BAPTA-1 AM)
was from Thermo Fisher Scientific (Carlsbad, CA, USA). PAC-1-FITC and anti-CD62P-APC were obtained from BD Bioscience (San Jose,
CA, USA). Anti-AKT, phospho (p)AKT-Ser473, pGSK3β-Ser9 and pPKC-Ser substrates were from Cell Signaling Technology
(Danvers, MA, USA).
Mitrugno A., Rigg R.A., Laschober N.B., Ngo A.T., Pang J., Williams C.D., Aslan J.E, & McCarty O.J. (2017). Potentiation of TRAP-6-induced platelet dense granule release by blockade of P2Y12 signaling with MRS2395. Platelets, 29(4), 383-394.
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