Api test strips

Manufactured by bioMérieux

Sourced in France

API test strips are a type of laboratory equipment manufactured by bioMérieux. They are used to identify and classify bacterial species. The strips contain a series of dehydrated biochemical tests that are inoculated with a bacterial sample. The resulting patterns of reactions are then compared to a reference database to determine the identity of the bacteria.

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Lab products found in correlation

Vitek 2, by bioMérieux (1 mentions) Microflex lt sh, by Bruker (1 mentions) Specific antisera, by Bio-Rad (1 mentions)

Spelling variants of this product

API 20E test strips (28 citations) API tests (10 citations) API strips (9 citations) API 20E biochemical test strips (9 citations) API 20E test identification test strips (5 citations) API 20 E strip test (2 citations) API 20 NE test strips (2 citations) API®ZYM test strips (2 citations) API 50CHL test strips (2 citations) Api-Coryne test strips (2 citations)

4 protocols using api test strips

Comparative Microbial Identification in Clinical Samples

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Clinical specimens were collected and cultured according to standard practice. Blood culture bottles (aerobic) were incubated for up to five days in an automated system (Bactec, Becton-Dickinson, USA). Other samples were incubated on media allowing growth of aerobic, anaerobic and fastidious organisms and checked daily. Positive specimens were randomly allocated to identification by either MALDITOF-MS (Microflex LT/SH, Bruker, Germany, library DB4613) or conventional diagnostics. For the MALDITOF-MS arm, positive blood culture media or colonies from plates were sub-cultured onto blood agar until growth could be observed. (Supplementary Fig. 1) Colonies were then analysed by MALDITOF-MS twice daily, a result was considered positive identification if it gave a score ≥2.00. In the control arm, methods for identification included: Gram-staining, API test strips, VITEK2 (bioMérieux, Marcy l’Étoile, France) and other tests as per standard operating procedures. All media were commercially sourced.Fig. 1

Trial flow.

^a2 patients randomised to MALDITOF had identification by routine methods. They were analysed as per their randomisation arm (i.e. included in MALDITOF group).

Fig. 1

Nadjm B., Dat V.Q., Campbell J.I., Dung V.T., Torre A., Tu N.T., Van N.T., Trinh D.T., Lan N.P., Trung N.V., Hang N.T., Hoi L.T., Baker S., Wolbers M., Chau N.V., Van Kinh N., Thwaites G.E., van Doorn H.R, & Wertheim H.F. (2019). A randomised controlled trial of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDITOF-MS) versus conventional microbiological methods for identifying pathogens: Impact on optimal antimicrobial therapy of invasive bacterial and fungal infections in Vietnam. The Journal of Infection, 78(6), 454-460.

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Blood Culture Identification Protocol

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A volume of 1–2 mL blood, dependent on age, was inoculated into 20 mL tryptic soy broth with sodium polyanethole sulfonate (SPS). All blood bottles were examined daily and subcultured at 24 hours, 48 hours, and 7 days after incubation or if turbid, on examination.16 (link) Subcultures were onto 5% sheep blood agar and chocolate agar incubated in a candle jar and MacConkey agar incubated in air for 48 hours (all media; Oxoid, Basingstoke, United Kingdom; blood bottles, stoppers and caps supplied by PN Laboratories, Bangkok, Thailand). Bacterial isolates were identified by standard methods, including biochemical test using API test strips (bioMérieux, Marcy l'Etoile, France) and agglutination with specific antisera (Biorad, Hertfordshire, United Kingdom). All media and tests are subject to regular internal quality assessment.

Moore C.E., Pan-Ngum W., Wijedoru L.P., Sona S., Nga T.V., Duy P.T., Vinh P.V., Chheng K., Kumar V., Emary K., Carter M., White L., Baker S., Day N.P, & Parry C.M. (2014). Evaluation of the Diagnostic Accuracy of a Typhoid IgM Flow Assay for the Diagnosis of Typhoid Fever in Cambodian Children Using a Bayesian Latent Class Model Assuming an Imperfect Gold Standard. The American Journal of Tropical Medicine and Hygiene, 90(1), 114-120.

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Bacterial Phenotypic Profiling with APIs

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Analytical profile indices (APIs) from kit API 50CHB systems (BioMérieux, Marcy l’Etoile, France) were used to characterize the physiological and biochemical properties of the bacterial isolates. API panel test system substrate utilization tests were performed using the API 50CHB systems panel. API test strips were handled according to the manufacturer’s instructions (bioMerieux, Marcy l’Etoile, France). Stock cultures were streaked onto LB agar to obtain single colonies for each bacterial isolate. Bacterial colonies of each isolate were diluted in 0.85% NaCl solution. The amount of bacteria was adjusted to 1 McFarland, and 200 ml of this solution was transferred into each well of the panels. To prevent contamination from the air, the wells were filled with mineral oil, and incubated at 25°C. Readings were recorded after 48 h.

Han J.H., Shim H., Shin J.H, & Kim K.S. (2015). Antagonistic Activities of Bacillus spp. Strains Isolated from Tidal Flat Sediment Towards Anthracnose Pathogens Colletotrichum acutatum and C. gloeosporioides in South Korea. The Plant Pathology Journal, 31(2), 165-175.

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Bacterial Isolation and Identification

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Inoculums from the lungs, spleen, liver, kidney, and intracardiac clot were taken with a sterilized loop and plated onto Blood Agar and MacConkey Agar plates (IZSLT, Roma, Italy), and incubated at 25 °C for 36 h and at 37 °C for 24 h. Developed bacterial colonies were examined for taxonomical analyses according to Bergey’s Manual of Determinative Bacteriology [22 ]. Briefly, morphological characteristics of bacterial colonies, Gram staining, and oxidase and catalase results were considered, while biochemical reactions were performed on API test strips (Biomerieux ^TM, Marcy-l’Etoile, France).

Iurescia M., Santini A., Montagnani M., Diaconu E.L., Stravino F., Agnelli D., Vergari E., Fichi G, & Eleni C. (2022). First Isolation of Yarrowia lipolytica in a Granulomatous Pneumonia of a Spectacled Caiman, Caiman crocodilus Linnaeus, 1758. Pathogens, 11(11), 1255.

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